| First Author | Fukagawa T | Year | 2001 |
| Journal | EMBO J | Volume | 20 |
| Issue | 16 | Pages | 4603-17 |
| PubMed ID | 11500386 | Mgi Jnum | J:71073 |
| Mgi Id | MGI:2149153 | Doi | 10.1093/emboj/20.16.4603 |
| Citation | Fukagawa T, et al. (2001) CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells. EMBO J 20(16):4603-17 |
| abstractText | CENP-H has recently been discovered as a constitutive component of the centromere that co-localizes with CENP-A and CENP-C throughout the cell cycle. The precise function, however, remains poorly understood. We examined the role of CENP-H in centromere function and assembly by generating a conditional loss-of-function mutant in the chicken DT40 cell line. In the absence of CENP-H, cell cycle arrest at metaphase, consistent with loss of centromere function, was observed. Immunocytochemical analysis of the CENP-H-deficient cells demonstrated that CENP-H is necessary for CENP-C, but not CENP-A, localization to the centromere. These findings indicate that centromere assembly in vertebrate cells proceeds in a hierarchical manner in which localization of the centromere-specific histone CENP-A is an early event that occurs independently of CENP-C and CENP-H. |
