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Publication : In vivo imaging of tracheal epithelial cells in mice during airway regeneration.

First Author  Kim JK Year  2012
Journal  Am J Respir Cell Mol Biol Volume  47
Issue  6 Pages  864-8
PubMed ID  22984086 Mgi Jnum  J:286201
Mgi Id  MGI:6402338 Doi  10.1165/rcmb.2012-0164OC
Citation  Kim JK, et al. (2012) In vivo imaging of tracheal epithelial cells in mice during airway regeneration. Am J Respir Cell Mol Biol 47(6):864-8
abstractText  Many human lung diseases, such as asthma, chronic obstructive pulmonary disease, bronchiolitis obliterans, and cystic fibrosis, are characterized by changes in the cellular composition and architecture of the airway epithelium. Intravital fluorescence microscopy has emerged as a powerful approach in mechanistic studies of diseases, but it has been difficult to apply this tool for in vivo respiratory cell biology in animals in a minimally invasive manner. Here, we describe a novel miniature side-view confocal probe capable of visualizing the epithelium in the mouse trachea in vivo at a single-cell resolution. We performed serial real-time endotracheal fluorescence microscopy in live transgenic reporter mice to view the three major cell types of the large airways, namely, basal cells, Clara cells, and ciliated cells. As a proof-of-concept demonstration, we monitored the regeneration of Clara cells over 18 days after a sulfur dioxide injury. Our results show that in vivo tracheal microscopy offers a new approach in the study of altered, regenerating, or metaplastic airways in animal models of lung diseases.
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