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Publication : Agouti C57BL/6N embryonic stem cells for mouse genetic resources.

First Author  Pettitt SJ Year  2009
Journal  Nat Methods Volume  6
Issue  7 Pages  493-5
PubMed ID  19525957 Mgi Jnum  J:149352
Mgi Id  MGI:3848338 Doi  10.1038/nmeth.1342
Citation  Pettitt SJ, et al. (2009) Agouti C57BL/6N embryonic stem cells for mouse genetic resources. Nat Methods :1-3
abstractText  We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
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