| Type | MGI:General | Description | after 3.5 d in culture, the ~45% of homozygous embryos harvested at E3.5 that fail to hatch comprise a decompacted, loosely adherent aggregate of rounded cells within the zona pellucida after 3.5 d in culture, the ~32% of homozygous embryos harvested at E3.5 that hatched late have adhered to the substrate, but their ICM are small and disorganized, composed of loosely adherent cells, and their trophoblast cells exhibit elongated extensions ~23% of cultured homozygous mutant embryos harvested at E3.5 hatch normally and adhere to the substrate by day 2, but at day 3.5 of culture, small degenerating clumps of ICM cells remain associated with the zona pellucida and trophoblast cells exhibit elongated extensions outgrowths of hatched homozygous mutant blastocysts harvested at E3.5 and cultured in vitro for 3.5 d exhibit cellular degeneration and disruption of intercellular adhesion, in association with dispersal of the normally plasma membrane-localized junction proteins E-cadherin and beta-catenin |
