| Type | MGI:General | Description | at 16 hrs after 20 or 35 mg/kg kainic acid administration, homozygotes, but not wild-type controls, display induction of Jun mRNA and the proapoptotic factor BAX, as well as fragmented DNA in CA3 cells and an increased number of TUNEL-positive cells, suggesting hippocampal cell death by apoptosis the extension of BAX labeling and tissue damage in the CA3 subfield are larger than that of TUNEL staining, indicating progressive apoptotic hippocampal cell death after kainic acid-induced seizures cell loss is detected only in the CA3 subfield but not in other regions of the hippocampus an extensive gliosis is observed in the CA3 subfield by GFAP immunohistochemistry the extent of CA3 cell damage following treatment with 35 mg/kg kainic acid is greater than that observed with 20 mg/kg, indicating a dose-dependent toxic effect; a higher number of TUNEL-positive cells and a larger extension of reactive gliosis are additionally observed in homozygotes treated with 35 mg/kg at 12 hrs after 35 mg/kg kainic acid administration, homozygotes show a stronger Jun induction in the hippocampus and cerebral cortex than wild-type controls, correlating with the occurrence of hippocampal cell death |
