mice infected intracranially with a sublethal, gliatrophic, monoclonal antibody-derived variant of mouse hepatitis virus strain JHM (JHMV) 2.2v-1 develop more severe paralytic disease than wild-type mice and do not show signs of recovery by 14 days post infection (dpi) JHMV-infected mice show higher viral loads within the central nervous system at 5 dpi and show no viral control until 10 dpi, showing a modest reduction at 14 dpi the numbers of viral antigen+ cells in JHMV-infected mice are increased and foci are more widely distributed throughout the brain, the numbers of infected cells in white matter are increased, and viral dissemination to gray matter is seen JHMV-infected mice show diminished recruitment of inflammatory cells during early infection, showing less pronounced perivascular inflammation in the CNS, allowing for viral dissemination
