First Author | Zarlenga DS | Year | 1995 |
Journal | Biochim Biophys Acta | Volume | 1270 |
Issue | 2-3 | Pages | 215-7 |
PubMed ID | 7727547 | Mgi Jnum | J:24951 |
Mgi Id | MGI:72666 | Doi | 10.1016/0925-4439(95)00042-3 |
Citation | Zarlenga DS, et al. (1995) Enzymatic amplification and molecular cloning of cDNA encoding the small and large subunits of bovine interleukin 12. Biochim Biophys Acta 1270(2-3):215-7 |
abstractText | cDNA generated from stimulated abomasal lymph node cells was used to amplify and clone the 35 kDa and 40 kDa subunits of bovine interleukin 12 (IL-12) using primers derived from semi-conserved regions between human and mouse IL-12 sequences. The deduced amino acid sequence of the 40 kDa subunit demonstrated 84.4% and 67.6% homology with human and mouse sequences, respectively. The deduced sequence of the 35 kDa subunit exhibited comparable similarities to the human 35 kDa subunit (82.2%) but differed significantly (58.6%) from mouse-derived sequences. |