| First Author | Khlebodarova TM | Year | 1988 |
| Journal | Genomics | Volume | 2 |
| Issue | 3 | Pages | 185-8 |
| PubMed ID | 3397057 | Mgi Jnum | J:9256 |
| Mgi Id | MGI:57719 | Doi | 10.1016/0888-7543(88)90001-8 |
| Citation | Khlebodarova TM, et al. (1988) The mink proopiomelanocortin gene: characterization of cDNA and chromosomal localization. Genomics 2(3):185-8 |
| abstractText | A cDNA library from the mink pituitary was screened using as probe a synthetic oligodeoxyribonucleotide, 5'-TTCATGACCTCCGA-3', corresponding to the endorphin region of bovine proopiomelanocortin (POMC) cDNA. As a result, several clones containing inserts complementary to POMC mRNA were identified. The sequence of one of the fragments (585 bp, 65% of the total length of mRNA) was determined. A high degree of homology (over 80%) among the primary structures of sequences from mink, man, and bovine cDNA POMC was established. With the cloned mink cDNA fragment as probe, the DNAs from mink-Chinese hamster hybrid clones were studied. The results of segregation analysis of mink POMC sequences and mink chromosomes in the mink-Chinese hamster panel allowed us to assign the POMC gene to mink chromosome 11. |
