| First Author | Kawakami T | Year | 1986 |
| Journal | Mol Cell Biol | Volume | 6 |
| Issue | 12 | Pages | 4195-201 |
| PubMed ID | 3099169 | Mgi Jnum | J:31022 |
| Mgi Id | MGI:78331 | Doi | 10.1128/mcb.6.12.4195 |
| Citation | Kawakami T, et al. (1986) Isolation and oncogenic potential of a novel human src-like gene. Mol Cell Biol 6(12):4195-201 |
| abstractText | We have isolated cDNA molecules representing the complete coding sequence of a new human gene which is a member of the src family of oncogenes. Nucleotide sequence analysis revealed that this gene, termed slk, encoded a 537-residue protein which was 86% identical to the chicken proto-oncogene product, p60c-src, over a stretch of 191 amino acids at its carboxy terminus. In contrast, only 6% amino acid homology was observed within the amino-terminal 82 amino acid residues of these two proteins. It was possible to activate slk as a transforming gene by substituting approximately two-thirds of the slk coding sequence for an analogous region of the v-fgr onc gene present in Gardner-Rasheed feline sarcoma virus. The resulting hybrid protein molecule expressed in transformed cells demonstrated protein kinase activity with specificity for tyrosine residues. |
