| First Author | Harbers K | Year | 1982 |
| Journal | Nucleic Acids Res | Volume | 10 |
| Issue | 8 | Pages | 2521-37 |
| PubMed ID | 6281733 | Mgi Jnum | J:6767 |
| Mgi Id | MGI:55239 | Doi | 10.1093/nar/10.8.2521 |
| Citation | Harbers K, et al. (1982) Infectivity and structure of molecular clones obtained from two genetically transmitted Moloney leukemia proviral genomes. Nucleic Acids Res 10(8):2521-37 |
| abstractText | The Mov-2 and Mov-10 substrains of mice, each carrying Moloney leukemia virus (= M-MuLV) in their germ line at the Mov-2 and Mov-10 locus, respectively, do occasionally at a later age (Mov-2) or not at all (Mov-10) activate infectious virus. The M-MuLV proviruses with flanking mouse sequences corresponding to the Mov-2 and Mov-10 locus, respectively, were molecularly cloned. Restriction enzyme analysis revealed no major deletions or insertions in the proviral genomes of the Mov-2 and Mov-10 locus. Both cloned DNAs induced XC plaques in a transfection assay. The specific infectivity, however, was very low and 3T3 cells transfected with the Mov-2 or Mov-10 clone did not produce infectious virus. Removing part of the 5' cellular sequences from the Mov-10 clone did not increase the infectivity. The results suggest that the M-MuLV integrated at the Mov-2 and Mov-10 locus carry a mutation which prevents synthesis of infectious virus but permits XC plaque induction by partial genome expression or synthesis of non-infectious particles. |
