| First Author | Arden B | Year | 1992 |
| Journal | Eur J Immunol | Volume | 22 |
| Issue | 5 | Pages | 1287-91 |
| PubMed ID | 1533592 | Mgi Jnum | J:1006 |
| Mgi Id | MGI:49538 | Doi | 10.1002/eji.1830220525 |
| Citation | Arden B (1992) Diversity of novel recombining elements suggests developmentally programmed expression of the T cell receptor alpha/delta locus. Eur J Immunol 22(5):1287-91 |
| abstractText | During fetal ontogeny, the first wave of gamma delta T lymphocytes appears in the thymus at day 14 of gestation assembling predominantly T cell receptors (TcR) with V gamma 3 and V delta 1. To identify V delta gene segments that are transcribed at day 16, subsequent to the first wave of V delta 1 expression, delta chain cDNA was amplified by the anchored polymerase chain reaction with single-sided specificity for C delta. Unexpectedly, most of the cDNA clones do not contain V gene segments. In some cDNA clones an alternative splice from the leader exon to the C delta exon has deleted the whole variable region exon. In other cDNA clones, multiple non-V-like elements are juxtaposed to the D delta 2 and J delta 1 gene segments. A large number of these diverse elements appear to be rearranged in fetal thymocytes, bringing V alpha gene segments located upstream of the recombining element into proximity to the J alpha locus. It is proposed that these rearrangements make irreversible the commitment to the TcR alpha beta lineage and determine a programmed read out of different clusters of V alpha gene segments. |
