| First Author | Ledermann B | Year | 1991 |
| Journal | Exp Cell Res | Volume | 197 |
| Issue | 2 | Pages | 254-8 |
| PubMed ID | 1959560 | Mgi Jnum | J:2753 |
| Mgi Id | MGI:51275 | Doi | 10.1016/0014-4827(91)90430-3 |
| Citation | Ledermann B, et al. (1991) Establishment of a germ-line competent C57BL/6 embryonic stem cell line. Exp Cell Res 197(2):254-8 |
| abstractText | Embryonic stem (ES) cell lines have been derived from blastocysts of the inbred mouse strain C57BL/6. The highest frequencies of ES cell colonies were observed when blastocysts were explanted directly onto growth-arrested feeder layers of 5637 human bladder carcinoma cells in the presence of conditioned medium. One of the male ES cell lines tested (BL/6-III) was shown to be karyotypically stable and germ-line competent when introduced into BALB/c host blastocysts. These results demonstrate that ES cell lines from inbred mouse strains other than 129/Sv may be used as vectors to introduce selected mutations into the germ-line of mice. |
