| First Author | Tamaki K | Year | 1994 |
| Journal | J Invest Dermatol | Volume | 103 |
| Issue | 3 | Pages | 290-4 |
| PubMed ID | 7521373 | Mgi Jnum | J:20174 |
| Mgi Id | MGI:68286 | Doi | 10.1111/1523-1747.ep12394437 |
| Citation | Tamaki K, et al. (1994) Migration of Thy-1+ dendritic epidermal cells (Thy-1+DEC): Ly48 and TNF-alpha are responsible for the migration of Thy-1+DEC to the epidermis. J Invest Dermatol 103(3):290-4 |
| abstractText | Thy-1+ dendritic epidermal cells (Thy-1+DEC) are mainly T cells that express T-cell receptor gamma and delta chains with limited diversity of gamma delta, mainly gamma 3 delta 1; such gamma 3 delta 1 TCR-bearing Thy-1+DEC originate from day 16 fetal thymic cells. To understand the migratory capability of Thy-1+DEC, we developed an in vitro model, using skin organ culture. First, emigration of Thy-1+DEC from the epidermis was examined. Ear skin from C3H/He mice was separated into two parts and incubated for 3 d with dermal side down. Thy-1+DEC emigrated from the epidermis into the dermis and then migrated out of the skin into the culture medium. Next, immigration of Thy-1+DEC into the epidermis was examined. Thy-1+DEC were depleted in vivo by daily application of clobetazole propionate solution topically onto the ears of C3H/He mice. Seven days later, ear skin was harvested, separated, and cultured with the dermal side up with syngeneic epidermal cell suspensions with a migration chamber for 3 d. It was found that 1) Thy-1+DEC immigrated into the Thy-1+DEC depleted epidermis as well as into untreated epidermis, and 2) the migratory capability of Thy-1+DEC was directly proved by a biolabeling technique with PKH-26. Blocking studies with various antibodies revealed that leukosialin (S11 monoclonal antibodies) and TNF alpha were important for Thy-1+DEC migration. Thus, Thy-1+DEC retain the potential for migration in vitro, and leukosialin and TNF alpha are partially responsible for the migration of Thy-1+DEC into the epidermis. |
