| First Author | Sasaki H | Year | 1994 |
| Journal | Cell | Volume | 76 |
| Issue | 1 | Pages | 103-15 |
| PubMed ID | 8287471 | Mgi Jnum | J:16438 |
| Mgi Id | MGI:64519 | Doi | 10.1016/0092-8674(94)90176-7 |
| Citation | Sasaki H, et al. (1994) HNF-3 beta as a regulator of floor plate development. Cell 76(1):103-15 |
| abstractText | The transcription factor gene HNF-3 beta is expressed in the ventral midline of the mouse embryonic neural tube, including the floor plate, a structure important for dorsoventral patterning and axonal guidance. To assess HNF-3 beta function, the gene has been ectopically expressed in the midbrain/hindbrain of transgenic embryos using an En-2 promoter/enhancer. By 18.5 days postcoitum, transgenic brains show a range of abnormalities, including absent inferior colliculus and reduced cerebellum. Earlier, several genes normally expressed in the floor plate (BMP-1, Steel factor, and HNF-3 alpha) are induced within the same ectopic dorsal domain as HNF-3 beta, and autoactivation of the endogenous HNF-3 beta is observed. Conversely, expression of the dorsal gene Pax-3 is suppressed. Ectopic dorsal neuronal differentiation and abnormal dorsal axonal projections are also seen. These results suggest that HNF-3 beta is an important regulator of floor plate development in vivo. |
