| First Author | Terhune PG | Year | 1998 |
| Journal | Pancreas | Volume | 16 |
| Issue | 1 | Pages | 6-12 |
| PubMed ID | 9436856 | Mgi Jnum | J:45279 |
| Mgi Id | MGI:1194725 | Doi | 10.1097/00006676-199801000-00002 |
| Citation | Terhune PG, et al. (1998) Evaluation of p53 mutation in pancreatic acinar cell carcinomas of humans and transgenic mice. Pancreas 16(1):6-12 |
| abstractText | Mutation of the p53 tumor suppressor gene is found in a large number of exocrine pancreatic tumors. The majority of these tumors is of the ductal cell phenotype. We examined 12 human acinar cell carcinomas and 42 transgenic mouse carcinomas (including 36 acinar cell tumors, four islet cell tumors, and two liver metastases of primary acinar cell tumors) for evidence of p53 mutation. Immunohistochemistry was used to identify p53 protein in tumor sections. To evaluate p53 exons 5-8, heteroduplex analysis was used on formalin-fixed, paraffin-embedded human tumor DNA, and single-strand conformation polymorphism analysis was used on frozen mouse tumor DNA. No molecular evidence of p53 mutation was found in any of the tumor DNAs and immunohistochemical data were regarded as negative. This study provides evidence that acinar cell carcinogenesis in both humans and transgenic mice is independent of p53 mutation. |
