| First Author | Liminga M | Year | 1999 |
| Journal | Biochim Biophys Acta | Volume | 1437 |
| Issue | 2 | Pages | 124-35 |
| PubMed ID | 10064896 | Mgi Jnum | J:54148 |
| Mgi Id | MGI:1334146 | Doi | 10.1016/s1388-1981(99)00004-9 |
| Citation | Liminga M, et al. (1999) cDNA cloning of 15-lipoxygenase type 2 and 12-lipoxygenases of bovine corneal epithelium. Biochim Biophys Acta 1437(2):124-35 |
| abstractText | Bovine corneal epithelium contains arachidonate 12- and 15-lipoxygenase activity, while human corneal epithelium contains only 15-lipoxygenase activity. Our purpose was to identify the corneal 12- and 15-lipoxygenase isozymes. We used cDNA cloning to isolate the amino acid coding nucleotide sequences of two bovine lipoxygenases. The translated sequence of one lipoxygenase was 82% identical with human 15-lipoxygenase type 2 and 75% identical with mouse 8-lipoxygenase, whereas the other translated nucleotide sequence was 87% identical with human 12-lipoxygenase of the platelet type. Expression of 15-lipoxygenase type 2 and platelet type 12-lipoxygenase mRNAs were detected by Northern analysis. In addition to these two lipoxygenases, 12-lipoxygenase of leukocyte (tracheal) type was detected by polymerase chain reaction (PCR), sequencing, and Northern analysis. Finally, PCR and sequencing suggested that human corneal epithelium contains 15-lipoxygenase types 1 and 2. |
