| First Author | Kyuwa S | Year | 2003 |
| Journal | Exp Anim | Volume | 52 |
| Issue | 1 | Pages | 81-4 |
| PubMed ID | 12638242 | Mgi Jnum | J:82272 |
| Mgi Id | MGI:2651975 | Doi | 10.1538/expanim.52.81 |
| Citation | Kyuwa S, et al. (2003) Differences between BALB/c and C57BL/6 mice in mouse hepatitis virus replication in primary hepatocyte culture. Exp Anim 52(1):81-4 |
| abstractText | We previously showed that an intraperitoneal infection with mouse hepatitis virus (MHV) resulted in acute hepatic failure accompanying extremely elevated viral growth in the liver in interferon-gamma-deficient BALB/c (BALB-GKO), but not C57BL/6 (B6-GKO) mice. To examine the basis of the strain difference against MHV infection in interferon-gamma-deficient mice, viral replication in primary hepatocyte cultures from BALB/c and B6 mice with or without the IFN-gamma gene was compared in vitro. The MHV replication in BALB/c hepatocytes with or without the IFN-gamma gene was significantly higher than that in B6 hepatocytes with or without the IFN-gamma gene, suggesting that there is a strain difference in MHV replication in hepatocytes. Since a significant difference in MHV replication in hepatocytes was not observed between wild type and IFN-gamma-deficient mice of the same genetic background, the phenomenon is thought to be independent of IFN-gamma. However, pretreatment of hepatocytes with recombinant mouse interferon-gamma inhibited MHV replication in a dose-dependent fashion. The results are discussed with respect to the pathology of MHV infection in mice with or without the IFN-gamma gene. |
