begin to lose weight by 3 to 5 days after infection with the Urbani strain of SARS-CoV concomitant with weight loss mice become lethargic and develop breathing difficulties viral titers in the lungs are 5 to 10-fold higher at 2 days post infection with SARS-Cov compared to non-transgenic littermates SARS-CoV is detectable in the brain of line 2 mice at 2 and 3 days but not 1 day post infection in comparison virus is not detectable in the brain until 9 days post infection in non-transgenic littermates inflammatory responses and signs of lung injury are increased at 2 days post infection compared to non-transgenic littermates at 4 days post infection non-transgenic littermates show resolution of most inflammatory responses, in contrast transgenic mice show perivascular and peribronchial inflammation, hemorrhage, and congestion of alveolar septa patchy, intense neutrophilic infiltrates are seen in the lungs of some mice
