| First Author | Chumley MJ | Year | 2000 |
| Journal | J Immunol | Volume | 164 |
| Issue | 9 | Pages | 4586-93 |
| PubMed ID | 10779761 | Mgi Jnum | J:107365 |
| Mgi Id | MGI:3620898 | Doi | 10.4049/jimmunol.164.9.4586 |
| Citation | Chumley MJ, et al. (2000) A VH11V kappa 9 B cell antigen receptor drives generation of CD5+ B cells both in vivo and in vitro. J Immunol 164(9):4586-93 |
| abstractText | B lymphocytes can be divided into different subpopulations, some with distinctive activation requirements and probably mediating specialized functions, based on surface phenotype and/or anatomical location, but the origins of most of these populations remain poorly understood. B cells constrained by transgenesis to produce an Ag receptor derived from a conventional (B-2) type cell develop a B-2 phenotype, whereas cells from mice carrying a B-1-derived receptor acquire the B-1 phenotype. In this study transgenic enforced expression of a B cell receptor (mu/kappa) originally isolated from a CD5+ (B-1a) B cell generates B-1 phenotype cells in bone marrow cultures that show a distinctive B-1 function, survival in culture. Despite their autoreactivity, we find no evidence for receptor editing or that the paucity of B-2 cells is the result of tolerance-induced selection. Finally, Ca2+ mobilization studies reveal a difference between transgenic B-1 cells in spleen and peritoneal cavity, with cells in spleen much more responsive to anti-B cell receptor cross-linking. We discuss these results in terms of specificity vs lineage models for generation of distinctive B cell subpopulations. |
