| First Author | Shyu YC | Year | 2014 |
| Journal | Dev Cell | Volume | 28 |
| Issue | 4 | Pages | 409-22 |
| PubMed ID | 24576425 | Mgi Jnum | J:316274 |
| Mgi Id | MGI:6835022 | Doi | 10.1016/j.devcel.2014.01.007 |
| Citation | Shyu YC, et al. (2014) Tight regulation of a timed nuclear import wave of EKLF by PKCtheta and FOE during Pro-E to Baso-E transition. Dev Cell 28(4):409-22 |
| abstractText | Erythropoiesis is a highly regulated process during which BFU-E are differentiated into RBCs through CFU-E, Pro-E, PolyCh-E, OrthoCh-E, and reticulocyte stages. Uniquely, most erythroid-specific genes are activated during the Pro-E to Baso-E transition. We show that a wave of nuclear import of the erythroid-specific transcription factor EKLF occurs during the Pro-E to Baso-E transition. We further demonstrate that this wave results from a series of finely tuned events, including timed activation of PKCtheta, phosphorylation of EKLF at S68 by P-PKCtheta(S676), and sumoylation of EKLF at K74. The latter EKLF modifications modulate its interactions with a cytoplasmic ankyrin-repeat-protein FOE and importinbeta1, respectively. The role of FOE in the control of EKLF nuclear import is further supported by analysis of the subcellular distribution patterns of EKLF in FOE-knockout mice. This study reveals the regulatory mechanisms of the nuclear import of EKLF, which may also be utilized in the nuclear import of other factors. |
