| First Author | Isakov D | Year | 2011 |
| Journal | Eur J Immunol | Volume | 41 |
| Issue | 12 | Pages | 3513-28 |
| PubMed ID | 21928282 | Mgi Jnum | J:179510 |
| Mgi Id | MGI:5302592 | Doi | 10.1002/eji.201141453 |
| Citation | Isakov D, et al. (2011) Lack of IL-7 and IL-15 signaling affects interferon-gamma production by, more than survival of, small intestinal intraepithelial memory CD8+ T cells. Eur J Immunol 41(12):3513-28 |
| abstractText | Survival of antigen-specific CD8(+) T cells in peripheral lymphoid organs during viral infection is known to be dependent predominantly on IL-7 and IL-15. However, little is known about a possible influence of tissue environmental factors on this process. To address this question, we studied survival of memory antigen-specific CD8(+) T cells in the small intestine. Here, we show that 2 months after vaccinia virus infection, B8R(20-27) /H2-K(b) tetramer(+) CD8(+) T cells in the small intestinal intraepithelial (SI-IEL) layer are found in mice deficient in IL-15 expression. Moreover, SI-IEL and lamina propria lymphocytes do not express the receptor for IL-7 (IL-7Ralpha/CD127). In addition, after in vitro stimulation with B8R(20-27) peptide, SI-IEL cells do not produce high amounts of IFN-gamma neither at 5 days nor at 2 months postinfection (p.i.). Importantly, the lack of IL-15 was found to shape the functional activity of antigen-specific CD8(+) T cells, by narrowing the CTL avidity repertoire. Taken together, these results reveal that survival factors, as well as the functional activity, of antigen-specific CD8(+) T cells in the SI-IEL compartments may markedly differ from their counterparts in peripheral lymphoid tissues. |
