| First Author | Cheng TC | Year | 1995 |
| Journal | Proc Natl Acad Sci U S A | Volume | 92 |
| Issue | 2 | Pages | 561-5 |
| PubMed ID | 7831329 | Mgi Jnum | J:22523 |
| Mgi Id | MGI:70386 | Doi | 10.1073/pnas.92.2.561 |
| Citation | Cheng TC, et al. (1995) Activation of the myogenin promoter during mouse embryogenesis in the absence of positive autoregulation. Proc Natl Acad Sci U S A 92(2):561-5 |
| abstractText | Myogenin, a member of the MyoD family of helix-loop-helix proteins, can induce myogenesis in a wide range of cell types. In addition to activating muscle structural genes, members of the MyoD family can autoactivate their own and cross-activate one another's expression in transfected cells. This has led to the hypothesis that autoregulatory loops among these factors provide a mechanism for amplifying and maintaining the muscle-specific gene expression program in vivo. Here, we make use of myogenin-null mice to directly test this hypothesis. To investigate whether the myogenin protein autoregulates the myogenin gene during embryogenesis, we introduced a myogenin-lacZ transgene into mice harboring a null mutation at the myogenin locus. Despite a severe deficiency of skeletal muscle in myogenin-null neonates, the myogenin-lacZ transgene was expressed normally in myogenic cells throughout embryogenesis. These results show that myogenin is not required for regulation of the myogenin gene and argue against the existence of a myogenin autoregulatory loop in the embryo. |
