| First Author | Kishi H | Year | 1995 |
| Journal | J Immunol | Volume | 155 |
| Issue | 2 | Pages | 568-77 |
| PubMed ID | 7608536 | Mgi Jnum | J:26758 |
| Mgi Id | MGI:74190 | Doi | 10.4049/jimmunol.155.2.568 |
| Citation | Kishi H, et al. (1995) A novel cell surface antigen, immature thymocyte antigen-1, is involved in the differentiation of murine thymocytes. J Immunol 155(2):568-77 |
| abstractText | A mAb, 1-23, which recognizes a novel cell surface Ag on immature murine thymocytes (designated as IMT-1 for immature thymocyte antigen-1) was prepared. IMT-1 was found to be expressed on 40 to 50% of CD4-8- double negative (DN), 5 to 10% of CD4-8+, and 5 to 20% of CD4+8+ double positive (DP) thymocytes in adult mice, but not expressed on CD4+8- thymocytes or peripheral T lymphocytes. It was not expressed on either nonlymphoid cell lines or thymic stromal cells. In subsets of DN thymocytes, IMT-1 was detected on 40 to 50% of the heat-stable Ag+, 15% of CD44+25+, 70% of CD44-25+, and 70% of CD44-25- populations, whereas it was not detected on heat-stable Ag- and CD44+25- populations. IMT-1+ thymocytes expressed low levels of or no CD3 molecules. In fetal thymuses, IMT-1 was expressed on a minor population of thymocytes at day 14.5 and 15.5 of gestation. However, at day 16.5 of gestation, a majority of DN as well as DP thymocytes became IMT-1-positive. Addition of the 1-23 Ab to the fetal thymus organ culture relatively increased CD8+ SP thymocytes. These results show that IMT-1 is expressed during the late DN stage as well as the early DP stage of thymocyte maturation and suggest the possible involvement of IMT-1 in thymocyte differentiation. |
