| First Author | Uchida S | Year | 1997 |
| Journal | Biochem Biophys Res Commun | Volume | 232 |
| Issue | 1 | Pages | 65-8 |
| PubMed ID | 9125153 | Mgi Jnum | J:39406 |
| Mgi Id | MGI:86788 | Doi | 10.1006/bbrc.1997.6236 |
| Citation | Uchida S, et al. (1997) Regulation of aquaporin-2 gene transcription by GATA-3. off. Biochem Biophys Res Commun 232(1):65-8 |
| abstractText | To evaluate the functional role of GATA motifs in the 5'-flanking region of a kidney-specific AQP-2 water channel gene, we sought to isolate a GATA factor(s) expressed in collecting ducts and determined the role on the AQP-2 promoter. Two cDNAs encoding GATA factors were isolated from rat kidney, whose sequences were highly homologous with human GATA-2 and -3. Reverse-transcription PCR using dissected nephron segments revealed that rat GATA-3 but not GATA-2 was expressed in collecting ducts, thus indicating that GATA-3 could interact with GATA motifs in the AQP-2 promoter. Transactivation experiments utilizing the rat GATA-3 expression vector indicated that rat GATA-3 increased the AQP-2 promoter activity about fourfold. These results indicated that GATA motifs in the 5'-flanking region of the hAQP-2 gene were functional cis-elements and that GATA-3 in collecting ducts may be one of the important regulators of AQP-2 expression in vivo. |
