| First Author | Chen L | Year | 2016 |
| Journal | FEBS Lett | Volume | 590 |
| Issue | 23 | Pages | 4263-4274 |
| PubMed ID | 27714787 | Mgi Jnum | J:238631 |
| Mgi Id | MGI:5823296 | Doi | 10.1002/1873-3468.12448 |
| Citation | Chen L, et al. (2016) Integrins and heparan sulfate proteoglycans on hepatic stellate cells (HSC) are novel receptors for HSC-derived exosomes. FEBS Lett 590(23):4263-4274 |
| abstractText | Exosomes mediate intercellular microRNA delivery between hepatic stellate cells (HSC), the principal fibrosis-producing cells in the liver. The purpose of this study was to identify receptors on HSC for HSC-derived exosomes, which bind to HSC rather than to hepatocytes. Our findings indicate that exosome binding to HSC is blocked by treating HSC with RGD, EDTA, integrin alphav or beta1 siRNAs, integrin alphavbeta3 or alpha5beta1 neutralizing antibodies, heparin, or sodium chlorate. Furthermore, exosome cargo delivery and exosome-regulated functions in HSC, including expression of fibrosis- or activation-associated genes and/or miR-214 target gene regulation, are dependent on cellular integrin alphavbeta3, integrin alpha5beta1, or heparan sulfate proteolgycans (HSPG). Thus, integrins and HSPG mediate the binding of HSC-derived exosomes to HSC as well as the delivery and intracellular action of the exosomal payload. |
