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Publication : MicroRNA-223 is essential for maintaining functional β-cell mass during diabetes through inhibiting both FOXO1 and SOX6 pathways.

First Author  Li Y Year  2019
Journal  J Biol Chem Volume  294
Issue  27 Pages  10438-10448
PubMed ID  31118273 Mgi Jnum  J:281106
Mgi Id  MGI:6368758 Doi  10.1074/jbc.RA119.007755
Citation  Li Y, et al. (2019) MicroRNA-223 is essential for maintaining functional beta-cell mass during diabetes through inhibiting both FOXO1 and SOX6 pathways. J Biol Chem 294(27):10438-10448
abstractText  The initiation and development of diabetes are mainly ascribed to the loss of functional beta-cells. Therapies designed to regenerate beta-cells provide great potential for controlling glucose levels and thereby preventing the devastating complications associated with diabetes. This requires detailed knowledge of the molecular events and underlying mechanisms in this disorder. Here, we report that expression of microRNA-223 (miR-223) is up-regulated in islets from diabetic mice and humans, as well as in murine Min6 beta-cells exposed to tumor necrosis factor alpha (TNFalpha) or high glucose. Interestingly, miR-223 knockout (KO) mice exhibit impaired glucose tolerance and insulin resistance. Further analysis reveals that miR-223 deficiency dramatically suppresses beta-cell proliferation and insulin secretion. Mechanistically, using luciferase reporter gene assays, histological analysis, and immunoblotting, we demonstrate that miR-223 inhibits both forkhead box O1 (FOXO1) and SRY-box 6 (SOX6) signaling, a unique bipartite mechanism that modulates expression of several beta-cell markers (pancreatic and duodenal homeobox 1 (PDX1), NK6 homeobox 1 (NKX6.1), and urocortin 3 (UCN3)) and cell cycle-related genes (cyclin D1, cyclin E1, and cyclin-dependent kinase inhibitor P27 (P27)). Importantly, miR-223 overexpression in beta-cells could promote beta-cell proliferation and improve beta-cell function. Taken together, our results suggest that miR-223 is a critical factor for maintaining functional beta-cell mass and adaptation during metabolic stress.
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