| First Author | Ortiz C | Year | 2020 |
| Journal | Sci Adv | Volume | 6 |
| Issue | 26 | Pages | eabb3446 |
| PubMed ID | 32637622 | Mgi Jnum | J:356107 |
| Mgi Id | MGI:7762199 | Doi | 10.1126/sciadv.abb3446 |
| Citation | Ortiz C, et al. (2020) Molecular atlas of the adult mouse brain. Sci Adv 6(26):eabb3446 |
| abstractText | Brain maps are essential for integrating information and interpreting the structure-function relationship of circuits and behavior. We aimed to generate a systematic classification of the adult mouse brain based purely on the unbiased identification of spatially defining features by employing whole-brain spatial transcriptomics. We found that the molecular information was sufficient to deduce the complex and detailed neuroanatomical organization of the brain. The unsupervised (non-expert, data-driven) classification revealed new area- and layer-specific subregions, for example in isocortex and hippocampus, and new subdivisions of striatum. The molecular atlas further supports the characterization of the spatial identity of neurons from their single-cell RNA profile, and provides a resource for annotating the brain using a minimal gene set-a brain palette. In summary, we have established a molecular atlas to formally define the spatial organization of brain regions, including the molecular code for mapping and targeting of discrete neuroanatomical domains. |
