| First Author | Van Heyningen V | Year | 1989 |
| Journal | Cytogenet Cell Genet | Volume | 51 |
| Pages | 1095 (Abstr.) | Mgi Jnum | J:12488 |
| Mgi Id | MGI:60733 | Citation | Van Heyningen V, et al. (1989) Related calcium binding proteins map to the same sub-region of chromosome 1q and to an extended region of synteny on mouse chromosome 3. Cytogenet Cell Genet 51:1095 (Abstr.) |
| abstractText | Full text of Abstract: Related calcium binding proteins map to the same sub-region of chromosome 1q and to an extended region of synteny on mouse chromosome 3. (A2223) V. van Heyningen, E Emslie, JR Dorin. MRC Human Genetics Unit, Crewe Road, Edinburgh, UK. The serum protein cystic fibrosis associated antigen (CFAg), present at elevated level in CF homozygotes and heterozygotes, is now known to consist of two distinct but related subunits (calgranulins A (CAGA) and B (CAGB)). Both show homology with the calcium binding protein S100. Using cDNA probes (isolated from a chronic myeloid leukemia-derived library) for both subunits we have confirmed the assignment of CAGA to the q12-q21 subregion of chromosome 1 and shown that CAGB co-segregates with it in our somatic cell hybrid DNA panel. cDNA probes for the related genes calcyclin (CACY) and a mouse placental protein 18A2, suggested name CAPL) were kindly supplied by R. Baserga and D. Linzer, respectively. This enabled us to confirm the in situ hybridization of CACY to Chromosome 1 q21-q25 (Ferrari et al., J. Biol. Chem. (1987) 262, 8325-8332) and demonstrate that both genes co-segregate with CAGA and CAGB. Southern blot analysis of TaqI digested DNA from BXD recombinant inbred strain mice (gift of G. Bulfield) distinguished the parental CAPL alleles and the segregation pattern allowed the assignment of CAPL to murine chromosome 3. The segregation of CAPL mirrored exactly the pattern for a further member of the S100 family, the p11 protein (mouse symbol Cal1l) which was mapped to mouse chromosome 3 (Saris et al., 262: 10663-10671, 1987). |
