Nuclear pore complex protein NUP214 (also known as Protein LONO1 and Protein EMBRYO DEFECTIVE 1011) is a component of the nuclear pore complex in Arabidopsis, required for mature mRNA export from the nucleus to the cytoplasm, essential for normal embryogenesis and seed viability []. It is important during early embryogenesis, being involved in the first asymmetrical cell division of the zygote and regulates the number and planes of cell divisions required for generating the normal embryo proper and suspensor, apical-basal axis, cotyledons and meristem [, ].
CRM1 is the major nuclear export receptor. During translocation through the nuclear pore, transport complexes transiently interact with phenylalanine-glycine (FG) repeats of multiple nucleoporins. On the cytoplasmic side of the nuclear pore, CRM1 tightly interacts with the nucleoporin Nup214. Nup214 binds to N- and C-terminal regions of CRM1, thereby clamping CRM1 in a closed conformation andstabilizing the export complex. This entry represents an FG repeat region within the C terminus of Nup214 which is required for its interaction with CRM1 [].
