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Search results 104401 to 104500 out of 119218 for Was

Category restricted to GXDExpression (x)

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Categories

Category: GXDExpression
Type Details Score
GXD Expression
Probe: MGI:5430034
Assay Type: Immunohistochemistry
Annotation Date: 2024-02-13
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3288326
Pattern: Single cells
Stage: TS26
Assay Id: MGI:7582026
Age: embryonic day 18.5
Image: 6F/H
Note: Coexpressed with Myo6 in some cells, even though the gross morphology of the inner ears was highly hypoplastic.
Specimen Label: 6F/H
Detected: true
Specimen Num: 7
GXD Expression
Probe: MGI:7645375
Assay Type: Immunohistochemistry
Annotation Date: 2024-06-05
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3288319
Pattern: Regionally restricted
Stage: TS19
Assay Id: MGI:7645397
Age: embryonic day 11.5
Image: 5O*
Note: Expression was detected in the thickened sensory epithelium of the cochlea, especially in the more ventral region.
Specimen Label: 5O*
Detected: true
Specimen Num: 24
GXD Expression
Probe: MGI:7645375
Assay Type: Immunohistochemistry
Annotation Date: 2024-06-05
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3288319
Pattern: Regionally restricted
Stage: TS19
Assay Id: MGI:7645397
Age: embryonic day 11.5
Image: 5P*
Note: Expression was detected in the thickened sensory epithelium of the cochlea, especially in the more ventral region.
Specimen Label: 5P*
Detected: true
Specimen Num: 25
GXD Expression
Probe: MGI:7645375
Assay Type: Immunohistochemistry
Annotation Date: 2024-06-05
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3288319
Pattern: Regionally restricted
Stage: TS19
Assay Id: MGI:7645397
Age: embryonic day 11.5
Image: 5R*
Note: Expression was detected in the thickened sensory epithelium of the cochlea, especially in the more ventral region.
Specimen Label: 5R*
Detected: true
Specimen Num: 27
GXD Expression
Probe: MGI:7645374
Assay Type: Immunohistochemistry
Annotation Date: 2024-06-05
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3288319
Pattern: Regionally restricted
Stage: TS19
Assay Id: MGI:7645396
Age: embryonic day 11.5
Image: 5H
Note: Expression was detected in a small population of posterior epithelial cells that lay at the end of the developing cochlea.
Specimen Label: 5H
Detected: true
Specimen Num: 21
GXD Expression
Probe: MGI:7645374
Assay Type: Immunohistochemistry
Annotation Date: 2024-06-05
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3288319
Pattern: Regionally restricted
Stage: TS19
Assay Id: MGI:7645396
Age: embryonic day 11.5
Image: 5I
Note: Expression was detected in a small population of posterior epithelial cells that lay at the end of the developing cochlea.
Specimen Label: 5I
Detected: true
Specimen Num: 22
GXD Expression
Probe: MGI:7645374
Assay Type: Immunohistochemistry
Annotation Date: 2024-06-05
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3288319
Pattern: Regionally restricted
Stage: TS19
Assay Id: MGI:7645396
Age: embryonic day 11.5
Image: 5J
Note: Expression was detected in a small population of posterior epithelial cells that lay at the end of the developing cochlea.
Specimen Label: 5J
Detected: true
Specimen Num: 23
GXD Expression
Probe: MGI:2446523
Assay Type: RNA in situ
Annotation Date: 2003-01-02
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1666518
Pattern: Regionally restricted
Stage: TS18
Assay Id: MGI:2447297
Age: embryonic day 11.0
Image: 3L
Note: Expression was detected in differentiating neurons and in 2 rows of discrete periodic bodies along the medial-dorsal axis.
Specimen Label: 3L
Detected: true
Specimen Num: 21
GXD Expression  
Probe: MGI:4946633
Assay Type: RNA in situ
Annotation Date: 2011-04-15
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1666517
Pattern: Regionally restricted
Stage: TS17
Assay Id: MGI:4946661
Age: embryonic day 10.5
Note: Expression was specifically in the brain, spinal cord and peripheral ganglia, and no signal was seen outside neural tissue.
Specimen Label: 1A
Detected: true
Specimen Num: 1
GXD Expression
Probe: MGI:6112633
Assay Type: Immunohistochemistry
Annotation Date: 2018-01-29
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3294520
Pattern: Regionally restricted
Stage: TS20
Assay Id: MGI:6112646
Age: embryonic day 12.5
Image: 3C
Note: Expression in interdigital tissues. authors state protein expression pattern was essentially similar to that of RNA expression.
Specimen Label: 3C
Detected: true
Specimen Num: 3
GXD Expression  
Probe: MGI:4403730
Assay Type: RNA in situ
Annotation Date: 2024-08-16
Strength: Weak
Sex: Not Specified
Emaps: EMAPS:3294520
Pattern: Regionally restricted
Stage: TS20
Assay Id: MGI:7709792
Age: embryonic day 12.5
Note: Expression was reduced; expression did not reach the tip and appears to be weakly expressed in proximal interdigital regions.
Specimen Label: 2/S2N hl
Detected: true
Specimen Num: 4
GXD Expression  
Probe: MGI:7716720
Assay Type: RNA in situ
Annotation Date: 2024-09-12
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3294521
Pattern: Regionally restricted
Stage: TS21
Assay Id: MGI:7716772
Age: embryonic day 13.5
Note: Expression was clearly visible in the proximal interdigital region, and is absent from the most distal regions.
Specimen Label: S5I/I'
Detected: true
Specimen Num: 21
GXD Expression  
Probe: MGI:7716720
Assay Type: RNA in situ
Annotation Date: 2024-09-12
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3294521
Pattern: Regionally restricted
Stage: TS21
Assay Id: MGI:7716772
Age: embryonic day 13.5
Note: Expression was clearly visible in the proximal interdigital region, and is absent from the most distal regions.
Specimen Label: S5J/J'
Detected: true
Specimen Num: 22
GXD Expression  
Probe: MGI:7716720
Assay Type: RNA in situ
Annotation Date: 2024-09-12
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3294522
Pattern: Regionally restricted
Stage: TS22
Assay Id: MGI:7716772
Age: embryonic day 14.5
Note: Expression was clearly visible in the proximal interdigital region, and is absent from the most distal regions.
Specimen Label: S5K/K'
Detected: true
Specimen Num: 23
GXD Expression  
Probe: MGI:7716720
Assay Type: RNA in situ
Annotation Date: 2024-09-12
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3294522
Pattern: Regionally restricted
Stage: TS22
Assay Id: MGI:7716772
Age: embryonic day 14.5
Note: Expression was clearly visible in the proximal interdigital region, and is absent from the most distal regions.
Specimen Label: S5L/L'
Detected: true
Specimen Num: 24
GXD Expression
Probe: MGI:3812108
Assay Type: Immunohistochemistry
Annotation Date: 2008-10-27
Strength: Strong
Sex: Not Specified
Emaps: EMAPS:2828128
Pattern: Regionally restricted
Stage: TS28
Assay Id: MGI:3812114
Age: postnatal adult
Image: 2E
Note: Expression was strong over the kidney brush border membrane where it was concentrated toward the tips of the microvilli.
Specimen Label: 2E
Detected: true
Specimen Num: 5
GXD Expression
Probe: MGI:3812108
Assay Type: Immunohistochemistry
Annotation Date: 2008-10-27
Strength: Strong
Sex: Not Specified
Emaps: EMAPS:2828128
Pattern: Regionally restricted
Stage: TS28
Assay Id: MGI:3812114
Age: postnatal adult
Image: 2F
Note: Expression was strong over the kidney brush border membrane where it was concentrated toward the tips of the microvilli.
Specimen Label: 2F
Detected: true
Specimen Num: 6
GXD Expression
Probe: MGI:4460808
Assay Type: Immunohistochemistry
Annotation Date: 2010-07-19
Strength: Present
Sex: Not Specified
Emaps: EMAPS:2828128
Pattern: Not Specified
Stage: TS28
Assay Id: MGI:4460822
Age: postnatal adult
Image: 8A
Note: In the apical region of the cell labeling was detected on electron-dense tubules underlying the brush border.
Specimen Label: 8A
Detected: true
Specimen Num: 1
GXD Expression  
Probe: MGI:5819374
Assay Type: Immunohistochemistry
Annotation Date: 2017-01-31
Strength: Present
Sex: Female
Emaps: EMAPS:2828128
Pattern: Regionally restricted
Stage: TS28
Assay Id: MGI:5822833
Age: postnatal adult
Note: In the cortex of the adult kidney, expression was localized apically in the convoluted portion of the proximal tubules.
Specimen Label: 5E
Detected: true
Specimen Num: 5
GXD Expression
Probe: MGI:2386878
Assay Type: RNA in situ
Annotation Date: 2002-10-01
Strength: Present
Sex: Female
Emaps: EMAPS:1689528
Pattern: Regionally restricted
Stage: TS28
Assay Id: MGI:2386934
Age: postnatal adult
Image: 2D
Note: Expression was present at the inferior border of the posterior commissure between the thalamus and the superior colliculus.
Specimen Label: 2D
Detected: true
Specimen Num: 4
GXD Expression    
Assay Type: In situ reporter (knock in)
Annotation Date: 2004-01-23
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689519
Pattern: Regionally restricted
Stage: TS19
Assay Id: MGI:2686660
Age: embryonic day 11.5
Note: Expression was detected in endothelial cells of the midbrain and forebrain meninges at the sites where hemorrhages had occurred.
Specimen Label: 2C
Detected: true
Specimen Num: 2
GXD Expression
Probe: MGI:1277653
Assay Type: RNA in situ
Annotation Date: 2005-12-29
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689522
Pattern: Regionally restricted
Stage: TS22
Assay Id: MGI:3608676
Age: embryonic day 14.5
Image: 5A
Note: Expression was detected in a differentiating field in the presumable region of the olfactory bulb in the anterior rhinencephalon.
Specimen Label: 5A
Detected: true
Specimen Num: 7
GXD Expression
Probe: MGI:1277653
Assay Type: RNA in situ
Annotation Date: 2005-12-29
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689520
Pattern: Regionally restricted
Stage: TS20
Assay Id: MGI:3608676
Age: embryonic day 12.5
Image: 6A
Note: Expression was detected in the optoeminential zone including the eminentia thalami, supraoptic paraventricular area, anterior hypothalamus and posterior optic area.
Specimen Label: 6A
Detected: true
Specimen Num: 9
GXD Expression
Probe: MGI:1277653
Assay Type: RNA in situ
Annotation Date: 2005-12-29
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689522
Pattern: Regionally restricted
Stage: TS22
Assay Id: MGI:3608676
Age: embryonic day 14.5
Image: 6C
Note: Expression was detected in the optoeminential zone including the eminentia thalami, supraoptic paraventricular area, anterior hypothalamus and posterior optic area.
Specimen Label: 6C
Detected: true
Specimen Num: 10
GXD Expression
Probe: MGI:1331403
Assay Type: RNA in situ
Annotation Date: 2005-12-29
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689520
Pattern: Regionally restricted
Stage: TS20
Assay Id: MGI:3608677
Age: embryonic day 12.5
Image: 6B
Note: Expression was detected in the optoeminential zone including the eminentia thalami, supraoptic paraventricular area, anterior hypothalamus and posterior optic area.
Specimen Label: 6B
Detected: true
Specimen Num: 9
GXD Expression
Probe: MGI:1331403
Assay Type: RNA in situ
Annotation Date: 2005-12-29
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689522
Pattern: Regionally restricted
Stage: TS22
Assay Id: MGI:3608677
Age: embryonic day 14.5
Image: 6D
Note: Expression was detected in the optoeminential zone including the eminentia thalami, supraoptic paraventricular area, anterior hypothalamus and posterior optic area.
Specimen Label: 6D
Detected: true
Specimen Num: 10
GXD Expression
Probe: MGI:5440291
Assay Type: Immunohistochemistry
Annotation Date: 2012-11-01
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689522
Pattern: Regionally restricted
Stage: TS22
Assay Id: MGI:5440295
Age: embryonic day 14.5
Image: S2A/C
Note: Expression was absent from BetaIII-tubulin-positive maturing neurons in the mantle regions of the dorsal and ventral forebrain.
Specimen Label: S2A/C
Detected: true
Specimen Num: 22
GXD Expression
Probe: MGI:5440291
Assay Type: Immunohistochemistry
Annotation Date: 2012-11-01
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689522
Pattern: Regionally restricted
Stage: TS22
Assay Id: MGI:5440295
Age: embryonic day 14.5
Image: S2D/F
Note: Expression was absent from BetaIII-tubulin-positive maturing neurons in the mantle regions of the dorsal and ventral forebrain.
Specimen Label: S2D/F
Detected: true
Specimen Num: 23
GXD Expression
Probe: MGI:1334951
Assay Type: RNA in situ
Annotation Date: 2014-11-03
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689517
Pattern: Not Specified
Stage: TS17
Assay Id: MGI:5603423
Age: embryonic day 10.5
Image: 8U
Note: The Aln,Shh mutant embryos did not show the increased expression that was seen in Aln mutant embryos.
Specimen Label: 8U
Detected: true
Specimen Num: 11
GXD Expression    
Assay Type: In situ reporter (knock in)
Annotation Date: 2015-01-23
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689518
Pattern: Regionally restricted
Stage: TS18
Assay Id: MGI:5613575
Age: embryonic day 10.5
Note: Expression was not detected in the neuroepithelium. Detectable expression correlated with invading blood vessels from the perineural vascular plexus.
Specimen Label: not shown E10.5
Detected: true
Specimen Num: 17
GXD Expression
Probe: MGI:5752583
Assay Type: RNA in situ
Annotation Date: 2016-03-21
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689522
Pattern: Non-Uniform
Stage: TS22
Assay Id: MGI:5752590
Age: embryonic day 14.5
Image: 1C
Note: Expression in p5 was more prominent than at E10.5. Expression was detected in the developing pallial-sub-pallial boundary.
Specimen Label: 1C
Detected: true
Specimen Num: 3
GXD Expression
Probe: MGI:1277653
Assay Type: RNA in situ
Annotation Date: 2016-11-29
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689519
Pattern: Not Specified
Stage: TS19
Assay Id: MGI:5810325
Age: embryonic day 11.5
Image: 4aB/D
Note: In the mutant, the caudal border of forebrain expression was expanded and abuts the choroid plexus of the fourth ventricle.
Specimen Label: 4aB/D
Detected: true
Specimen Num: 2
GXD Expression
Probe: MGI:5823270
Assay Type: Immunohistochemistry
Annotation Date: 2017-02-07
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689517
Pattern: Ubiquitous
Stage: TS17
Assay Id: MGI:5823276
Age: embryonic day 10.5
Image: 1D
Note: Co-localization with Tubb3, an immature neuronal marker, was noted in cells near to the basal surface of dorsal forebrain.
Specimen Label: 1D
Detected: true
Specimen Num: 4
GXD Expression
Probe: MGI:18808
Assay Type: RNA in situ
Annotation Date: 2021-02-10
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689517
Pattern: Regionally restricted
Stage: TS17
Assay Id: MGI:6505901
Age: embryonic day 10.5
Image: 10C
Note: Expression was reduced and confined to a small midline domain. Also expressed in the overlying non-neural tissues.
Specimen Label: 10C
Detected: true
Specimen Num: 1
GXD Expression
Probe: MGI:18808
Assay Type: RNA in situ
Annotation Date: 2021-02-10
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689517
Pattern: Regionally restricted
Stage: TS17
Assay Id: MGI:6505901
Age: embryonic day 10.5
Image: 10D
Note: Expression was lower at the midline and higher in bilateral domains. Also expressed in the overlying non-neural tissues.
Specimen Label: 10D
Detected: true
Specimen Num: 2
GXD Expression  
Probe: MGI:1275942
Assay Type: RNA in situ
Annotation Date: 2021-02-10
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689517
Pattern: Regionally restricted
Stage: TS17
Assay Id: MGI:6505906
Age: embryonic day 10.5
Note: Expression was lower at the midline and higher in bilateral domains. Also expressed in the overlying non-neural tissues.
Specimen Label: control not shown
Detected: true
Specimen Num: 1
GXD Expression  
Probe: MGI:1275942
Assay Type: RNA in situ
Annotation Date: 2021-02-10
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689517
Pattern: Regionally restricted
Stage: TS17
Assay Id: MGI:6505906
Age: embryonic day 10.5
Note: Expression was reduced and confined to a small midline domain. Also expressed in the overlying non-neural tissues.
Specimen Label: mutnat not shown
Detected: true
Specimen Num: 2
GXD Expression
Probe: MGI:7261292
Assay Type: Immunohistochemistry
Annotation Date: 2022-04-13
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689522
Pattern: Not Specified
Stage: TS22
Assay Id: MGI:7261324
Age: embryonic day 14.5
Image: 5C
Note: In this mutant there was a non-statistically significant decrease in the number of proliferating cells compared to wild-type.
Specimen Label: 5C
Detected: true
Specimen Num: 3
GXD Expression
Probe: MGI:7261292
Assay Type: Immunohistochemistry
Annotation Date: 2022-04-13
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689522
Pattern: Not Specified
Stage: TS22
Assay Id: MGI:7261324
Age: embryonic day 14.5
Image: 5D
Note: In this mutant there was a non-statistically significant decrease in the number of proliferating cells compared to wild-type.
Specimen Label: 5D
Detected: true
Specimen Num: 4
GXD Expression
Probe: MGI:7261292
Assay Type: Immunohistochemistry
Annotation Date: 2022-04-13
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689522
Pattern: Not Specified
Stage: TS22
Assay Id: MGI:7261324
Age: embryonic day 14.5
Image: 5G
Note: In this mutant there was a non-statistically significant decrease in the number of proliferating cells compared to wild-type.
Specimen Label: 5G
Detected: true
Specimen Num: 7
GXD Expression
Probe: MGI:7261292
Assay Type: Immunohistochemistry
Annotation Date: 2022-04-13
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689522
Pattern: Not Specified
Stage: TS22
Assay Id: MGI:7261324
Age: embryonic day 14.5
Image: 5H
Note: In this mutant there was a non-statistically significant decrease in the number of proliferating cells compared to wild-type.
Specimen Label: 5H
Detected: true
Specimen Num: 8
GXD Expression  
Probe: MGI:3689839
Assay Type: RNA in situ
Annotation Date: 2022-08-16
Strength: Strong
Sex: Not Specified
Emaps: EMAPS:1689520
Pattern: Regionally restricted
Stage: TS20
Assay Id: MGI:7329846
Age: embryonic day 12.5
Note: Highly expressed in the neopallial cortex of the forebrain, but was not observed in the rostral forebrain.
Specimen Label: 7
Detected: true
Specimen Num: 1
GXD Expression  
Probe: MGI:27272
Assay Type: RNA in situ
Annotation Date: 2023-02-08
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689526
Pattern: Regionally restricted
Stage: TS26
Assay Id: MGI:7432574
Age: embryonic day 18.5
Note: There was no expression in the dorsal region. Only a small collection of positive cells in the ventral region.
Specimen Label: 3A dorsal -/-
Detected: true
Specimen Num: 10
GXD Expression  
Probe: MGI:7432417
Assay Type: RNA in situ
Annotation Date: 2023-02-08
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689526
Pattern: Regionally restricted
Stage: TS26
Assay Id: MGI:7432579
Age: embryonic day 18.5
Note: There was no expression in the dorsal region. Only a small collection of positive cells in the ventral region.
Specimen Label: 3A dorsal -/-
Detected: true
Specimen Num: 10
GXD Expression  
Probe: MGI:7518767
Assay Type: RNA in situ
Annotation Date: 2023-08-30
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689517
Pattern: Regionally restricted
Stage: TS17
Assay Id: MGI:7522249
Age: embryonic day 10.5
Note: Expression in the bridge region between the hemispheres in the mutant was similar in intensity to control.
Specimen Label: S6F
Detected: true
Specimen Num: 4
GXD Expression  
Probe: MGI:7518768
Assay Type: RNA in situ
Annotation Date: 2023-08-30
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689517
Pattern: Regionally restricted
Stage: TS17
Assay Id: MGI:7522262
Age: embryonic day 10.5
Note: Expression in the bridge region between the hemispheres in the mutant was similar in intensity to control.
Specimen Label: S6D Dmrt5<-/->
Detected: true
Specimen Num: 2
GXD Expression  
Probe: MGI:7518767
Assay Type: RNA in situ
Annotation Date: 2023-08-30
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689517
Pattern: Regionally restricted
Stage: TS17
Assay Id: MGI:7522249
Age: embryonic day 10.5
Note: Expression in the bridge region between the hemispheres in the mutant was similar in intensity to control.
Specimen Label: S6C Dmrt5<-/->
Detected: true
Specimen Num: 2
GXD Expression
Probe: MGI:3581531
Assay Type: Immunohistochemistry
Annotation Date: 2023-11-10
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689526
Pattern: Regionally restricted
Stage: TS26
Assay Id: MGI:7547381
Age: embryonic day 18.5
Image: 3C Olig1/2-/-
Note: Only staining in vessel pericytes was unaffected. No positive cell bodies with recognizable synantocyte or bipolar morphology were seen.
Specimen Label: 3C Olig1/2-/-
Detected: true
Specimen Num: 16
GXD Expression
Probe: MGI:3581531
Assay Type: Immunohistochemistry
Annotation Date: 2023-11-10
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689526
Pattern: Regionally restricted
Stage: TS26
Assay Id: MGI:7547381
Age: embryonic day 18.5
Image: 3C Olig1/2-/-
Note: Only staining in vessel pericytes was unaffected. No positive cell bodies with recognizable synantocyte or bipolar morphology were seen.
Specimen Label: 3C Olig1/2-/-
Detected: true
Specimen Num: 16
GXD Expression
Probe: MGI:3690303
Assay Type: Immunohistochemistry
Annotation Date: 2006-12-11
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1616517
Pattern: Regionally restricted
Stage: TS17
Assay Id: MGI:3690320
Age: embryonic day 10.5
Image: 1C
Note: Expression was detected in transversely oriented fibers restricted to the lower third of the floor plate region.
Specimen Label: 1C
Detected: true
Specimen Num: 5
GXD Expression  
Assay Type: In situ reporter (knock in)
Annotation Date: 2008-08-06
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1694218
Pattern: Regionally restricted
Stage: TS18
Assay Id: MGI:3800526
Age: embryonic day 11.5
Image: 2f
Note: Expression was detected in the ventricular and mantle layers of the neuroepithelium, juxtaposed to the floor plate.
Specimen Label: 2f
Detected: true
Specimen Num: 4
GXD Expression
Probe: MGI:1339605
Assay Type: RNA in situ
Annotation Date: 1999-07-13
Strength: Strong
Sex: Not Specified
Emaps: EMAPS:1619817
Pattern: Not Specified
Stage: TS17
Assay Id: MGI:1339699
Age: embryonic day 10.5
Image: 11.C
Note: Strong expression was observed around the optic cup and in mesenchymal cells localized above the optic cup.
Specimen Label: 11.C
Detected: true
Specimen Num: 3
GXD Expression
Probe: MGI:3841615
Assay Type: Immunohistochemistry
Annotation Date: 2009-04-28
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1619817
Pattern: Regionally restricted
Stage: TS17
Assay Id: MGI:3841616
Age: embryonic day 10.5
Image: 1D,F
Note: Expression was detected at relatively similar levels in the presumptive neural retina, retinal pigmented epithelium, corneal epithelium and lens vesicle.
Specimen Label: 1D,F
Detected: true
Specimen Num: 2
GXD Expression
Probe: MGI:4353425
Assay Type: RNA in situ
Annotation Date: 2009-08-20
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1619820
Pattern: Regionally restricted
Stage: TS20
Assay Id: MGI:4354016
Age: embryonic day 12.5
Image: 2A
Note: Expression was detected in the developing ciliary body. No significant expression was detected in the central portion of the retina.
Specimen Label: 2A
Detected: true
Specimen Num: 1
GXD Expression
Probe: MGI:4353425
Assay Type: RNA in situ
Annotation Date: 2009-08-20
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1619824
Pattern: Regionally restricted
Stage: TS24
Assay Id: MGI:4354016
Age: embryonic day 16.5
Image: 2B
Note: Expression was detected in the developing ciliary body. No significant expression was detected in the central portion of the retina.
Specimen Label: 2B
Detected: true
Specimen Num: 2
GXD Expression  
Probe: MGI:5774841
Assay Type: Immunohistochemistry
Annotation Date: 2016-06-02
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1619816
Pattern: Regionally restricted
Stage: TS16
Assay Id: MGI:5774847
Age: embryonic day 10.5
Note: There was distinct staining of the connecting fibers in the interspace between the lens pit and optic cup.
Specimen Label: 1B
Detected: true
Specimen Num: 2
GXD Expression  
Probe: MGI:6272030
Assay Type: RNA in situ
Annotation Date: 2019-01-04
Strength: Strong
Sex: Not Specified
Emaps: EMAPS:1619820
Pattern: Regionally restricted
Stage: TS20
Assay Id: MGI:6272263
Age: embryonic day 12.5
Note: Expression was strong in all ocular tissues: the developing cornea, lens, neuroectoderm, choroid, sclera, lid and conjunctiva.
Specimen Label: 2A
Detected: true
Specimen Num: 1
GXD Expression  
Probe: MGI:6272030
Assay Type: RNA in situ
Annotation Date: 2019-01-04
Strength: Strong
Sex: Not Specified
Emaps: EMAPS:1619821
Pattern: Regionally restricted
Stage: TS21
Assay Id: MGI:6272263
Age: embryonic day 13.5
Note: Expression was strong in all ocular tissues: the developing cornea, lens, neuroectoderm, choroid, sclera, lid and conjunctiva.
Specimen Label: 2B
Detected: true
Specimen Num: 2
GXD Expression  
Probe: MGI:6272030
Assay Type: RNA in situ
Annotation Date: 2019-01-04
Strength: Strong
Sex: Not Specified
Emaps: EMAPS:1619822
Pattern: Regionally restricted
Stage: TS22
Assay Id: MGI:6272263
Age: embryonic day 14.5
Note: Expression was strong in all ocular tissues: the developing cornea, lens, neuroectoderm, choroid, sclera, lid and conjunctiva.
Specimen Label: 2C
Detected: true
Specimen Num: 3
GXD Expression  
Probe: MGI:6287694
Assay Type: RNA in situ
Annotation Date: 2019-04-17
Strength: Strong
Sex: Not Specified
Emaps: EMAPS:1619824
Pattern: Regionally restricted
Stage: TS24
Assay Id: MGI:6287738
Age: embryonic day 16.5
Note: Expression was strong in primary fiber cells in the center of the lens; it was undetectable elsewhere in the eye.
Specimen Label: 4C,D
Detected: true
Specimen Num: 3
GXD Expression
Probe: MGI:1353285
Assay Type: Immunohistochemistry
Annotation Date: 2000-03-01
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1614812
Pattern: Regionally restricted
Stage: TS12
Assay Id: MGI:1353295
Age: embryonic day 8.0
Image: 1D
Note: Expression was shifted caudally to the level of rhombomere 4 with expression in only a few cells in rhombomere 3.
Specimen Label: 1D
Detected: true
Specimen Num: 4
GXD Expression
Probe: MGI:1933980
Assay Type: RNA in situ
Annotation Date: 2001-05-23
Strength: Strong
Sex: Not Specified
Emaps: EMAPS:1614813
Pattern: Regionally restricted
Stage: TS13
Assay Id: MGI:1934382
Age: embryonic day 8.5
Image: 4C
Note: In rostral hindbrain, signal was strongest on basal side of epithelium. More caudally, strong labelling both apically and basally.
Specimen Label: 4C
Detected: true
Specimen Num: 9
GXD Expression
Probe: MGI:14720
Assay Type: RNA in situ
Annotation Date: 2001-06-21
Strength: Strong
Sex: Not Specified
Emaps: EMAPS:1614814
Pattern: Regionally restricted
Stage: TS14
Assay Id: MGI:2135640
Age: embryonic day 9.0
Image: 1C -/-
Note: Expression was strongly expressed in streams extending from the hindbrain into arches 2, 3, and 4 as in wild-type embryos.
Specimen Label: 1C -/-
Detected: true
Specimen Num: 2
GXD Expression
Probe: MGI:1275919
Assay Type: RNA in situ
Annotation Date: 2001-07-24
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1614814
Pattern: Patchy
Stage: TS14
Assay Id: MGI:2137070
Age: embryonic day 8.5
Image: 3. D.
Note: Expression was detected in a patchy fashion from the preotic sulcus to the level of the first somite.
Specimen Label: 3. D.
Detected: true
Specimen Num: 2
GXD Expression
Probe: MGI:1101193
Assay Type: RNA in situ
Annotation Date: 2002-04-19
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1614815
Pattern: Regionally restricted
Stage: TS15
Assay Id: MGI:2177927
Age: embryonic day 9.5
Image: 3G
Note: Authors report that expression in the dorsal neural tube was more ventral and lateral than that of Msx2.
Specimen Label: 3G
Detected: true
Specimen Num: 2
GXD Expression
Probe: MGI:1933980
Assay Type: RNA in situ
Annotation Date: 2001-05-23
Strength: Strong
Sex: Not Specified
Emaps: EMAPS:1614813
Pattern: Regionally restricted
Stage: TS13
Assay Id: MGI:1934382
Age: embryonic day 8.5
Image: 4D
Note: In rostral hindbrain, signal was strongest on basal side of epithelium. More caudally, strong labelling both apically and basally.
Specimen Label: 4D
Detected: true
Specimen Num: 10
GXD Expression  
Probe: MGI:5776188
Assay Type: Immunohistochemistry
Annotation Date: 2016-06-16
Strength: Strong
Sex: Not Specified
Emaps: EMAPS:1614815
Pattern: Regionally restricted
Stage: TS15
Assay Id: MGI:5776227
Age: embryonic day 9.5
Note: Expression was strong in the metencephalon primitive vesicle. There was no expression in the myelencephalon primitive vesicle.
Specimen Label: Table 2 E9.5
Detected: true
Specimen Num: 19
GXD Expression  
Probe: MGI:5776186
Assay Type: RNA in situ
Annotation Date: 2016-06-16
Strength: Strong
Sex: Not Specified
Emaps: EMAPS:1614815
Pattern: Regionally restricted
Stage: TS15
Assay Id: MGI:5776225
Age: embryonic day 9.5
Note: Expression was strong in the metencephalon primitive vesicle. There was no expression in the myelencephalon primitive vesicle.
Specimen Label: Table 2 E9.5
Detected: true
Specimen Num: 26
GXD Expression  
Probe: MGI:7437493
Assay Type: RNA in situ
Annotation Date: 2023-02-21
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1757120
Pattern: Regionally restricted
Stage: TS20
Assay Id: MGI:7437501
Age: embryonic day 11.5
Note: Expression was detected in the cochleovestibular ganglion only at the cochlear level and not at the utricular level.
Specimen Label: 3C
Detected: true
Specimen Num: 3
GXD Expression  
Probe: MGI:7437493
Assay Type: RNA in situ
Annotation Date: 2023-02-21
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1757120
Pattern: Regionally restricted
Stage: TS20
Assay Id: MGI:7437501
Age: embryonic day 11.5
Note: Expression was detected in the cochleovestibular ganglion only at the cochlear level and not at the utricular level.
Specimen Label: 3H
Detected: true
Specimen Num: 4
GXD Expression
Probe: MGI:2155933
Assay Type: RNA in situ
Annotation Date: 2004-03-25
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1752922
Pattern: Regionally restricted
Stage: TS22
Assay Id: MGI:3033435
Age: embryonic day 14.5
Image: 4D
Note: Expression was detected in the vibrissal follicles as in wild type, but there were fewer follicles in the mutant.
Specimen Label: 4D
Detected: true
Specimen Num: 4
GXD Expression  
Probe: MGI:2155933
Assay Type: RNA in situ
Annotation Date: 2004-03-25
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1752921
Pattern: Regionally restricted
Stage: TS21
Assay Id: MGI:3033435
Age: embryonic day 13.5
Note: Expression was detected in the vibrissal follicles as in wild type, but there were fewer follicles in the mutant.
Specimen Label: not shown
Detected: true
Specimen Num: 6
GXD Expression  
Probe: MGI:6116265
Assay Type: Immunohistochemistry
Annotation Date: 2018-02-15
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1752920
Pattern: Regionally restricted
Stage: TS20
Assay Id: MGI:6116462
Age: embryonic day 12.5
Note: Expression was in the maxillary part of the facial region associated to the basal cells of the primary vibrissae.
Specimen Label: 5E
Detected: true
Specimen Num: 5
GXD Expression  
Probe: MGI:3639884
Assay Type: RNA in situ
Annotation Date: 2006-08-04
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3267820
Pattern: Regionally restricted
Stage: TS20
Assay Id: MGI:3639919
Age: embryonic day 12.5
Note: Expression was increased in the climbing fiber neurons in the intramural migratory stream in the mutant compared to wild type.
Specimen Label: 6L
Detected: true
Specimen Num: 2
GXD Expression  
Assay Type: In situ reporter (knock in)
Annotation Date: 2010-03-30
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3267828
Pattern: Regionally restricted
Stage: TS28
Assay Id: MGI:4438119
Age: postnatal month 6
Image: 1A
Note: Authors report that expression was restricted to very few brainstem regions and was absent in other brain regions.
Specimen Label: 1A
Detected: true
Specimen Num: 1
GXD Expression  
Probe: MGI:4946653
Assay Type: RNA in situ
Annotation Date: 2011-04-15
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3267825
Pattern: Regionally restricted
Stage: TS25
Assay Id: MGI:4946668
Age: embryonic day 17.5
Note: Expression was present in the reticular formation but was very low or undetectable in the cochleovestibular nuclei.
Specimen Label: Table 2
Detected: true
Specimen Num: 5
GXD Expression
Probe: MGI:3531020
Assay Type: RNA in situ
Annotation Date: 2014-10-22
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3267824
Pattern: Regionally restricted
Stage: TS24
Assay Id: MGI:5588673
Age: embryonic day 16.5
Image: 3H
Note: Expression was detected in the nucleus ambiguus, the dorsal nucleus of the vagus, and the hypoglossal nucleus.
Specimen Label: 3H
Detected: true
Specimen Num: 10
GXD Expression  
Probe: MGI:3844915
Assay Type: Immunohistochemistry
Annotation Date: 2009-05-20
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1914323
Pattern: Not Specified
Stage: TS23
Assay Id: MGI:3844957
Age: embryonic day 15.5
Note: Authors state that expression in the bones, head, gut and heart was very similar to that seen at E13
Specimen Label: 1F
Detected: true
Specimen Num: 6
GXD Expression
Probe: MGI:5439748
Assay Type: RNA in situ
Annotation Date: 2012-10-29
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1914325
Pattern: Regionally restricted
Stage: TS25
Assay Id: MGI:5439842
Age: embryonic day 17.5
Image: 2B/b
Note: Expression was detected in the hypertrophic zones of the growth plates at both ends of the growing bone.
Specimen Label: 2B/b
Detected: true
Specimen Num: 2
GXD Expression
Probe: MGI:5645495
Assay Type: Immunohistochemistry
Annotation Date: 2015-07-29
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1914326
Pattern: Regionally restricted
Stage: TS26
Assay Id: MGI:5645513
Age: embryonic day 18.0
Image: 6J
Note: Expression was detected in mature chondrocytes, with virtually no expression in the proliferating zone or in the future resting zone.
Specimen Label: 6J
Detected: true
Specimen Num: 37
GXD Expression
Probe: MGI:5645493
Assay Type: Immunohistochemistry
Annotation Date: 2015-07-30
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1914326
Pattern: Regionally restricted
Stage: TS26
Assay Id: MGI:5645561
Age: embryonic day 18.0
Image: 6I
Note: Expression was detected in mature chondrocytes, with virtually no expression in the proliferating zone or in the future resting zone.
Specimen Label: 6I
Detected: true
Specimen Num: 27
GXD Expression
Probe: MGI:5475302
Assay Type: RNA in situ
Annotation Date: 2013-04-25
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1760023
Pattern: Regionally restricted
Stage: TS23
Assay Id: MGI:5475307
Age: embryonic day 15.0
Image: 2B
Note: Expression was detected in the rostral wall of the cochlear duct from which the Reissner's membrane will form.
Specimen Label: 2B
Detected: true
Specimen Num: 2
GXD Expression
Probe: MGI:7287407
Assay Type: Immunohistochemistry
Annotation Date: 2022-06-21
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1760021
Pattern: Regionally restricted
Stage: TS21
Assay Id: MGI:7287430
Age: embryonic day 13.5
Image: 1D
Note: Expression was gradually restricted to the prosensory domain, primarily in the medial half of the cochlear duct at the apex.
Specimen Label: 1D
Detected: true
Specimen Num: 4
GXD Expression
Probe: MGI:5466486
Assay Type: In situ reporter (knock in)
Annotation Date: 2022-09-12
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1760026
Pattern: Regionally restricted
Stage: TS26
Assay Id: MGI:7335244
Age: embryonic day 18.5
Image: 1B E18.5 Middle
Note: Expressed in inner pillar cells in the middle and basal turns. Expression was increased compared to that of E15.5.
Specimen Label: 1B E18.5 Middle
Detected: true
Specimen Num: 6
GXD Expression
Probe: MGI:5466486
Assay Type: In situ reporter (knock in)
Annotation Date: 2022-09-12
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1760026
Pattern: Regionally restricted
Stage: TS26
Assay Id: MGI:7335244
Age: embryonic day 18.5
Image: 1B E18.5 Basal
Note: Expressed in inner pillar cells in the middle and basal turns. Expression was increased compared to that of E15.5.
Specimen Label: 1B E18.5 Basal
Detected: true
Specimen Num: 7
GXD Expression
Probe: MGI:1289467
Assay Type: RNA in situ
Annotation Date: 2013-02-14
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3559312
Pattern: Regionally restricted
Stage: TS12
Assay Id: MGI:5463912
Age: embryonic day 8.0
Image: 3h
Note: In the anterior region, at the level of the midbrain/hindbrain, expression was found exclusively on the left.
Specimen Label: 3h
Detected: true
Specimen Num: 11
GXD Expression
Probe: MGI:1289467
Assay Type: RNA in situ
Annotation Date: 2013-02-14
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3559312
Pattern: Regionally restricted
Stage: TS12
Assay Id: MGI:5463912
Age: embryonic day 8.0
Image: 3i & 3m
Note: In the anterior region, at the level of the midbrain/hindbrain, expression was found exclusively on the left.
Specimen Label: 3i & 3m
Detected: true
Specimen Num: 12
GXD Expression  
Probe: MGI:6324615
Assay Type: RNA in situ
Annotation Date: 2019-07-24
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3559312
Pattern: Non-Uniform
Stage: TS12
Assay Id: MGI:6324616
Age: embryonic day 7.75
Note: Expressed largely in the columnar neuroepithelial cells. The signal was stronger in the parts of the cells facing the mesoderm.
Specimen Label: 1D
Detected: true
Specimen Num: 4
GXD Expression
Probe: MGI:15970
Assay Type: RNA in situ
Annotation Date: 2005-11-23
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1913523
Pattern: Graded
Stage: TS23
Assay Id: MGI:3605540
Age: embryonic day 15.5
Image: 3N Mut
Note: Expression was downregulated at the site of joints, but was otherwise continuously expressed in the central region.
Specimen Label: 3N Mut
Detected: true
Specimen Num: 9
GXD Expression  
Probe: MGI:7339043
Assay Type: RNA in situ
Annotation Date: 2022-09-23
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1801721
Pattern: Not Specified
Stage: TS21
Assay Id: MGI:7339147
Age: embryonic day 13.5
Note: Decreased BrdU incorporation was observed by osteogenic cells at the apical edge of the Runx2 expression domain.
Specimen Label: 3B
Detected: true
Specimen Num: 8
GXD Expression
Probe: MGI:3807267
Assay Type: RNA in situ
Annotation Date: 2008-09-24
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3568928
Pattern: Regionally restricted
Stage: TS28
Assay Id: MGI:3807475
Age: embryonic day 15.5
Image: 5D & E
Note: Expression was detected in trophoblast cells within the labyrinthine region. Signal was absent from spongiotrophoblast and trophoblast giant cells.
Specimen Label: 5D & E
Detected: true
Specimen Num: 3
GXD Expression  
Probe: MGI:3810810
Assay Type: RNA in situ
Annotation Date: 2008-10-16
Strength: Strong
Sex: Not Specified
Emaps: EMAPS:3568928
Pattern: Regionally restricted
Stage: TS28
Assay Id: MGI:3810820
Age: embryonic day 18.5
Note: Expression was intense in trophoblast giant cells and spongiotrophoblast cells from the junctional zone of the chorioallantoic placenta.
Specimen Label: 7B & C
Detected: true
Specimen Num: 1
GXD Expression
Probe: MGI:928202
Assay Type: RNA in situ
Annotation Date: 2009-07-29
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3568928
Pattern: Regionally restricted
Stage: TS28
Assay Id: MGI:3851515
Age: embryonic day 10.5
Image: 6A & 6B
Note: Expression was detected near the fetal-maternal junction. Positive cells were restricted to the zone immediately under the trophoblast giant cells.
Specimen Label: 6A & 6B
Detected: true
Specimen Num: 1
GXD Expression
Probe: MGI:928202
Assay Type: RNA in situ
Annotation Date: 2009-07-29
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3568928
Pattern: Regionally restricted
Stage: TS28
Assay Id: MGI:3851515
Age: embryonic day 12.5
Image: 6C
Note: Expression was detected only in a narrow band of cells situated immediately deep to the trophoblast giant cell layer.
Specimen Label: 6C
Detected: true
Specimen Num: 2
GXD Expression  
Probe: MGI:5463276
Assay Type: RNA in situ
Annotation Date: 2013-02-11
Strength: Present
Sex: Female
Emaps: EMAPS:3568928
Pattern: Regionally restricted
Stage: TS28
Assay Id: MGI:5463359
Age: postnatal adult
Note: Expression was detected in the sinus system in the maternal part of the placenta at E10.5 and E15.5.
Specimen Label: not shown
Detected: true
Specimen Num: 20
GXD Expression
Probe: MGI:6476914
Assay Type: Immunohistochemistry
Annotation Date: 2020-12-04
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3568928
Pattern: Not Specified
Stage: TS28
Assay Id: MGI:6477567
Age: embryonic day 8.5
Image: 4F Zfp281 KO
Note: In this mutant the positive cell population was small and formed only a thin laminar structure compared to controls.
Specimen Label: 4F Zfp281 KO
Detected: true
Specimen Num: 2
GXD Expression  
Probe: MGI:6476914
Assay Type: Immunohistochemistry
Annotation Date: 2020-12-04
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3568928
Pattern: Not Specified
Stage: TS28
Assay Id: MGI:6477567
Age: embryonic day 8.5
Note: In this mutant the positive cell population was small and formed only a thin laminar structure compared to controls.
Specimen Label: 4F KO
Detected: true
Specimen Num: 3
GXD Expression  
Probe: MGI:1935187
Assay Type: Immunohistochemistry
Annotation Date: 2020-12-04
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3568928
Pattern: Not Specified
Stage: TS28
Assay Id: MGI:6477569
Age: embryonic day 8.5
Note: In this mutant the positive cell population was small and formed only a thin laminar structure compared to controls.
Specimen Label: 4F KO
Detected: true
Specimen Num: 3
GXD Expression
Probe: MGI:1935187
Assay Type: Immunohistochemistry
Annotation Date: 2020-12-04
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3568928
Pattern: Not Specified
Stage: TS28
Assay Id: MGI:6477569
Age: embryonic day 8.5
Image: 4F Zfp281 KO
Note: In this mutant the positive cell population was small and formed only a thin laminar structure compared to controls.
Specimen Label: 4F Zfp281 KO
Detected: true
Specimen Num: 2
GXD Expression  
Probe: MGI:4946644
Assay Type: RNA in situ
Annotation Date: 2011-04-15
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1754925
Pattern: Regionally restricted
Stage: TS25
Assay Id: MGI:4946664
Age: embryonic day 17.5
Note: Expression was detected in the striatum ventricular zone/subventricular zone and was very low or undetectable in the striatum.
Specimen Label: Table 2
Detected: true
Specimen Num: 5