Expression was weak in the most dorsal region of the olfactory epithelium. MOR7-1-positive cells were much reduced in Lhx2-/- embryos compared to wild-type embryos (94.0 in Lhx2-/-, 918 in wild-type, n=2).
Expression was weak in the most dorsal region of the olfactory epithelium. MOR32-4-positive cells were much reduced in Lhx2-/- embryos compared to wild-type embryos (36.6 +/-20.0 in Lhx2-/-, 412 +/-31.2 in wild-type, n=3).
Expression was weak in the most dorsal region of the olfactory epithelium. MOR28-1-positive cells were much reduced in Lhx2-/- embryos compared to wild-type embryos (10.0 +/-10.0 in Lhx2-/-, 364 +/-164 in wild-type, n=3).
Expression was detected in the olfactory receptor neurons situated in several layers in the middle region of the neuroepithelium. Staining was absent from sustentacular and microvillar cells and globose and horizontal basal cells.
Expression was detected olfactory neurons in the middle region of the epithelium. Staining of the luminal surface in the region of the dendritic knobs is evident. Signal was absent from the fasciculate axons that pass through the basal lamina.
Subcellularly, staining was apical to the cell nucleus and also in the dendrite and dendritic knob of the olfactory neurons. No staining was seen in the axons from the basal region or in axon fascicles below the basal lamina.
Expression was detected in the nasal epithelium lining the lateral side of the lumen of the primitive nasal cavity. The signal was mainly regionalized and patchy among cells in the recesses of the olfactory epithelium.
Expression was detected in the ventral olfactory epithelium. Expression was much less than that seen in the developing brain. No expression was detected along olfactory axons or outside the epithelium.
Expression was detected in a region of immature basal neurons. Strong expression was detected in round cells extending a dendriitc process to the apical surface of the olfactory epithelium.
LacZ-stained cells were retricted to particular areas; activity was found only along the dorsal part of the septum extending into the dorsal recess and the tip of turbinates. Expressing cells were located in the dorsal zone of the epithelium.
Expression was strong in the nascent vascular endothelium of the visceral yolk sac. At the 8-somite stage, there were fewer Hhex-positive cells in the visceral yolk sac vascular plexus compared with flk-1 (Kdr) (see Assay MGI:1306925).
Expression was exclusively in the vascular system and was concentrated at the adherence junctions of endothelial cells. No round hematopoietic cells were immunopositive. No staining was seen in the extraluminal space.
Expressed around the enamel knots in complex patterns. Expressed in most parts of the forming first molar. The expression was absent in two distinct locations. Expressed in the area forming a valley separating the anterior and posterior cusp pairs.
Expression was restricted to the pre-pancreatic domain. The Sox2-positive domain, from which the stomach develops, formed a boundary with both the Pdx1+ and Sox9+ domains. Very few cells co-expressing Sox2, Pdx1, and Sox9 were observed at this boundary.
Significant decrease of primordial germ cells, compared with the wild-type. Chek2 deficiency did not rescue germ cell depletion, Although there was an increased number of germ cells, compared with the single mutants.
Expression was absent from the stromal cells that surround the ureter but was detected in the renal capsule and in the stromal cells surrounding the ureteric bud and metanephric mesenchyme.
No expression in epithelial cells lining the small single cyst. High expression in the epithelial cells lining multilocular and large cysts from mutant polycystic kidneys. Expression was very low in the surrounding normal tissue.
No expression in epithelial cells lining the small single cyst. High expression in the epithelial cells lining multilocular and large cysts from mutant polycystic kidneys. Expression was very low in the surrounding normal tissue.
Expression was detected in the cortical plate of the cingulate cortex, but not in the underlying deeper layers of the cingulate cortex, including the intermediate zone and germinal layer.
Expression was prominent in dorsal and lateral parts of the neocortex in somatosensory and auditory areas. A domain of low expression was noted, especially in layers 4 and 5, in extreme rostral neocortex.
Expression was prominent in dorsal and lateral parts of the neocortex in somatosensory and auditory areas. A domain of low expression was noted, especially in layers 4 and 5, in extreme rostral neocortex.
Graded expression was detected in superficial layers rostrally, but in intermediate parts of the neocortex, the expression declines rapidly to low or nondetectable. Graded low to high expression was noted in layer 5 between motor and somatosensory areas.
Expression was detected in two distinct high rostral to low caudal gradients across the neocortex; a gradient in superficial layers that extends farther caudally than a gradient within the deeper layers.
In Emx2 mutants, expression in layer 4 was detected across virtually the entire neocortex. Layer 5 expression was expanded throughout the intermediate parts of the neocortex and well into caudal neocortex.
At E13 the number of immunopositive radial glial cells was significantly reduced in the mutant pallium. Widespread cytoplasmic expression was observed in the mutant, which was rare in the control sections.
Immunofluorescence with cleaved Casp3 was used to mark apoptotic cells. TUNEL-positive cells marked cells in late stages of apoptosis. Of the cleaved Casp3+/TUNEL- cells in the E12 mutant pallium, 38% were also EdU+.
In the E12 mutant pallium, the vast majority (93.4%) of the cleaved Casp3+ cells were pyknotic. Of the nonpyknotic Casp3+ cells, 4.44% were Tubb3-immunopositive, which was not significantly higher than the control.
In the E12 mutant pallium, the vast majority (93.4%) of the cleaved Casp3+ cells were pyknotic. Of the nonpyknotic Casp3+ cells, 4.44% were Tubb3-immunopositive, which was not significantly higher than the control.
Elevated immunoreactivity was detected in the cytoplasm of most neocortical neurons. Expression pattern was observed throughout the cerebral cortex regardless of the cortical area (somatosensory, motor, or entorhinal cortex).
The percentage of BrdU-positive/Eomes-positive cells in the proliferative regions was significantly increased in the mutant. The total number of Eomes-positive cells was not different in control and knockout cortices.
In mutant embryos with two vesicles on one side, expression was detected mainly in the dorsal vesicle, with weaker and more restricted expression in the ventral one. The level and expression domain decreased compared to wild type.
In mutant embryos with two vesicles on one side, expression in the ventral vesicle was weaker and more restricted. The level and expression domain was decreased compared to wild type.
Expression was detected in 3 regions of the otic epithelium: 1. medial otic epithelium; 2. lateral region just beneath the beginning of the horizontal semicircular canal; 3. ventral region.
Expression was detected in the patch-like structure observed in the inner ears of mutant embryos. The expression domain was not of the pattern of maculae, cristae or the organ of Corti.
Expression was detected in the patch-like structure observed in the inner ears of mutant embryos. The expression domain was not of the pattern of maculae, cristae or the organ of Corti.
Increased number of positive epithelial cells (quantified in Fig 3C). In Sox2+ presumptive sensory epithelial cells, apoptosis seemed to be increased although the increase was not statistically significant (quantified in Fig 3H).
Neurons are clearly recognisable in the gut mesenchyme; they are starting to appear in the peri-aortic region. Presence of neurons in the mesentery was not observed but cannot be excluded.
Expression was exclusively in neural cell bodies; no other cell types were stained. Expression was in many regions of the limbic system: septum nucleus, horizontal & vertical limbs of the Diagonal band, hippocampus, amygdaloid nucleus, & habenula nucleus.
Expression was observed in ventral longitudinal stripes which extend along the hindbrain, midbrain, and continue into the ventral forebrain. The longitudinal stripes branch off to a region surrounding the zone limitans intrathalamica.
Expression was observed in ventral longitudinal stripes which extend along the hindbrain, midbrain, and continue into the ventral forebrain. The longitudinal stripes branch off to a region surrounding the zone limitans intrathalamica.
Expression was detected in post-mitotic mantle zones with only a few areas of negative staining. Higher expression levels were noted in the regions of the habenular commissure and the lateral ventricle.
Expression was detected in the medial septum and preoptic area of the basal forebrain. There was a significant increase in silver grain density at E19.5 compared to E14.5 and E16.5.
Expression was in 2 regions. Caudal- extends from the area of the suprachiasmatic nucleus through the hypothalamic cell cord and posterior entopeduncular area into the ventral thalamus. Rostral- from the lateral ganglionic eminence to the optic stalk.
Expression was in 2 regions. Caudal- extends from the area of the suprachiasmatic nucleus through the hypothalamic cell cord and posterior entopeduncular area into the ventral thalamus. Rostral- from the lateral ganglionic eminence to the optic stalk.
Expression was in 2 regions. Caudal- extends from the area of the suprachiasmatic nucleus through the hypothalamic cell cord and posterior entopeduncular area into the ventral thalamus. Rostral- from the lateral ganglionic eminence to the optic stalk.
Weak expression was found throughout the forebrain. Higher expression in the diencephalon had a posterior boundary in zona limitans intrathalamica, a rostral p4/p5 limit, and dorsally in the eminentia thalami as it entered the telencephalon.
Expression was detected in the reduced thalamocortical axons and in the mutant ectopic axon bundle in the anterior hypothalamic region dorsolateral to the optic chiasm and rostral to the zona incerta and lateral hypothalamus.
Expression was detected in the Telenecephalic Vesicles, dorsal spinal cord and the laterovertebral mesenchyme. The latervertebral mesenchyme is defined as the cells located between the prevertebrae and the dorsal root ganglia.
Expression was detected in a row of cells just ventral to the lumen of the neural tube and in radial processes running from the region of these cells to the pial surface.
Expression was detected in the anterior optic vesicle. The expression pattern of lacZ in the mutant is present in approximately half of the cells of the optic vesicle. The medially expanded retina comprises both lacZ-expressing and non-expressing cells.
Expression was detected in the anterior optic vesicle. The expression pattern of lacZ in the mutant is present in approximately half of the cells of the optic vesicle. The medially expanded retina comprises both lacZ-expressing and non-expressing cells.
High expression was detected in the epithelium and mesenchymal cells of the eyelid and in the groove between the eyelid and eyeball. Intense expression was also noted in the eyeball.
Expression was detected in all ocular tissues: the developing cornea, lens, neuroectoderm, choroid, sclera, and lid. Especially strong labeling was seen in the lens epithelium, neuroectoderm, surface ectoderm of the presumptive lid and conjunctiva.
Expression was detected in all ocular tissues: the developing cornea, lens, neuroectoderm, choroid, sclera, and lid. Especially strong labeling was seen in the lens epithelium, neuroectoderm, surface ectoderm of the presumptive lid and conjunctiva.
Expression was detected in all ocular tissues: the developing cornea, lens, neuroectoderm, choroid, sclera, and lid. Especially strong labeling was seen in the lens epithelium, neuroectoderm, surface ectoderm of the presumptive lid and conjunctiva.
Expression was seen in a band of staining, 2-3 cell layers thick, along the entire caudal-cranial axis of the pelvic urethra. It is not seen in the most cranial bladder basal urothelial cells (cells of the bladder apex).
Pax2 was expressed in the mid-hindbrain region in mutant embryos, but the expression domain in mutant embryos is smaller in size presumably due to the absence of the midbrain.
The expression domain of kreisler corresponding to the prospective r5/r6 terrotory was twice the size of the wild-type domain. Additionally, the sharp caudal limit characteristic of the wild-type domain was missing.
The expression domain of kreisler corresponding to the prospective r5/r6 terrotory was twice the size of the wild-type domain. Expression in the roof plate of the pre-otic rhombomeres was not affected.
The anterior Hoxc4 boundary did not coincide with a morphologically visible rhombomere junction, but was located posterior to anterior Hoxb4 boundary (at the junction of rhombomeres 6 and 7) by about one rhombomere length.
Highest signal just rostral to otic placodes; rostral and caudal to this, signal was strongest basally, and a medial unlabelled area was more extensive. A small group of midline supranotochordal cells was unlabelled.
Patchy staining along the basal surface of the neural tube. Staining around the otocyst and on the ventrolateral aspect of the adjacent (rhombencephalic) neural tube increased in intensity. The strongest reaction was found between the two structures.
Expression was strong in a small ventral midline patch around the mesencephalon/metencephalon junction and weak in a thin transverse band of cells at the anterior end of the hindbrain.
Expression was detected in rhombomere 4 and dorsal rhombomere 3. Below rhombomere 4, a transverse band of cells does not express GFP. Expression in the posterior hindbrain region was downregulated.
Highest signal just rostral to otic placodes; rostral and caudal to this, signal was strongest basally, and a medial unlabelled area was more extensive. A small group of midline supranotochordal cells was unlabelled.
Highest signal just rostral to otic placodes; rostral and caudal to this, signal was strongest basally, and a medial unlabelled area was more extensive. A small group of midline supranotochordal cells was unlabelled.
Expressed in the perikaryon and cell processes of the neuroepithelium. Cells containing protein but not mRNA were also observed in the neuroepithelium. Protein but not mRNA was detected in neurons in the mantle zone.
Expression was detected in the perikarya of the ganglion cell bodies and the bipolar processes peripherally and centrally. Expression was strong in precursor cells migrating from the epithelium across the basal lamina.
Expression was detected in the dorsal and posteroventral cochlear nucleus (CN). In the dorsal CN, small intensely stained cells below the ependymal surface were noted. Some giant and corn cells, and cells of the internal lamella were also stained.
Expression was detected in large cells in the fiber tracts from vermal sections and in the brainstem. As these cells were not observed with the 5'Nav2 probe, they appear to be expressing the short transcript.
Expression was detected in large cells in the fiber tracts from vermal sections and brainstem, as in wild type. As these cells were not observed with the 5'Nav2 probe, they appear to be expressing the short transcript.
Expression was seen in a band of staining, 2-3 cell layers thick, along the entire caudal-cranial axis of the pelvic urethra. It is not seen in the most cranial bladder basal urothelial cells (cells of the bladder apex).
Expression was detected in the apical surface of all epithelial cells. In the immature organ of Corti cells expression was only in the apical surface. Expression that overlapped with Ki-67 was found both in the apical and basal portion.
Mainly expressed in one row of Sox2-positive progenitor cells in the middle and basal turns of the organ of Corti, and no expression was observed in the apical turn.
Mainly expressed in one row of Sox2-positive progenitor cells in the middle and basal turns of the organ of Corti, and no expression was observed in the apical turn.
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn. Decreased expression compared with that of P3.
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn. Decreased expression compared with that of P3.
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn. Decreased expression compared with that of P3.
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn. Decreased expression compared with that of P3.
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn. Decreased expression compared with that of P3.
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn. Decreased expression compared with that of P3.
Expression is detected in the ventral domain of all rhombomeres, except rhombomere 1. It was strongest in r2 and r4, corresponding to the precursors of the trigeminal and facial motoneurons.
Expression is detected in the ventral domain of all rhombomeres, except rhombomere 1. It was strongest in r2 and r4, corresponding to the precursors of the trigeminal and facial motoneurons.
Expression in the mutant failed to extend apically, its apical tip rather regressed towards the basal side. In contrast, basal side extension did not appear to be affected in the mutant. The expression domain was smaller than the wild type.
Expression in the mutant failed to extend apically, its apical tip rather regressed towards the basal side. In contrast, basal side extension did not appear to be affected in the mutant. The expression domain was smaller than the wild type.
Expression in the mutant failed to extend apically, its apical tip rather regressed towards the basal side. In contrast, basal side extension did not appear to be affected in the mutant. The expression domain was smaller than the wild type.
Expression was detected in the spongiotrophoblast and the giant cells in the labyrinthine zone, but very few giant cells were detected on the fetal side of the placenta and none were detected in the chorioallantoic plate.
Expression was detected in the spongiotrophoblast and the giant cells in the labyrinthine zone, more giant cells were detected on the fetal side of the placenta than in the heterozygous control.
Expression was primarily in the striatal ventricular zone. Immunolabeled cells were pale or absent from the ventricular surface, and labeling was greater in the subventricular zone. Immunopositive neurons were scattered in the striatum.
Expressed in the progenitors of the ventricular zone, and also in the secondary proliferative population, which consists of nonepithelial progenitor cells located in a deeper subventricular zone. Expression was absent in the mitotic condensed chromosomes.
There is a matrix-like pattern of expression with small and evenly distributed patches devoid of staining. At the periphery there was partial coexpression, and at the central-most region there was a complementary expression pattern.
There is a matrix-like pattern of expression with small and evenly distributed patches devoid of staining. At the periphery there was partial coexpression, and at the central-most region there was a complementary expression pattern.
Positive mesencephalic dopaminergic axons grew towards the dorsal part of the striatum where low Efna5 expression was detected, whereas no positive axons crossed the most ventral and rostral part of the striatum expressing high Efna5 levels.
Expression was restricted to the ventricular segment of the linear heart tube and colocalized with Nkx2-5. Expression was not detected at the arterial or venous poles of the heart tube.
Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing hip, tibia and fibula.
Expression was detected in the cytoplasm of cells in the zone of hypertrophy and zone of ossification. Apoptotic chondrocytes in the zone of calcification show signal in the whole cell.
The length of the zone of proliferative chondrocytes expressing Sox9 was 25% shorter in the conditional mutant. Results suggest that Sox9 expression was lost in several layers of flat chondrocytes closest to the prehypertrophic chondrocytes.
regional expression detected in immature loop of Henle tubules close to the renal cortex; no expression was detected in immature loop of Henle tubules deep in the renal medulla
Staining was present in the cytoplasm. There was intense staining encircling the cell borders and margins; this was most intense near unopposed cell membrances, compared to cell membranes near adjacent cells.
Co-localization with Padi6 was observed at the non-opposed cortex regions of 4-cell embryos with less co-localization being observed throughout the cytoplasm. Nlrp5 (Mater) staining penetrated deeper into the cytoplasm compared with Padi6.
