Co-localization with Nlrp5 (Mater) was observed at the non-opposed cortex regions of 4-cell embryos with less co-localization being observed throughout the cytoplasm. Nlrp5 (Mater) staining penetrated deeper into the cytoplasm compared with Padi6.
Expression was detected in a hexagonal lattice on the lateral plasma membranes and the apical processes of the retinal pigmented epithelium. Expression co-localized with Ezr (Ezrin) on the apical processes.
Expression was detected in the plasma membranes. The highest concentration of label was observed at the apical surface of the cells in the area where two adjacent cells juxtapose. Apical processes were also heavily labeled.
There was a spatial dose-dependent inverse relationship between Pax6 and Sox9, with a significant negative correlation between the mRNA levels. A central to peripheral gradient was noted for Sox9 transcripts.
There was a spatial dose-dependent inverse relationship between Pax6 and Sox9, with a significant negative correlation between the mRNA levels. A central to peripheral gradient was noted for Sox9 transcripts.
There was a spatial dose-dependent inverse relationship between Pax6 and Sox9, with a significant negative correlation between the mRNA levels. A central to peripheral gradient was noted for Sox9 transcripts.
Expression is at the apical and basal membrane. Anterior to the ora serrata where the neural retina ends little or no labelling was seen on the apical surface after this point.
Abrupt loss of expression was evident in regions where a multi-cell layered epithelium was forming. The expression pattern within the pseudostratified epithelium was similar to that of normal retina, but apical concentration was lost.
The area showing a break in the presumptive outer limiting membrane correlated with a decrease in Yap1 expression. There was partial fusion when the duplicated retina abutted the original retina.
The area showing a break in the presumptive outer limiting membrane correlated with a decrease in Yap1 expression. There was partial fusion when the duplicated retina abutted the original retina.
In the mutant, expression was not found in the dorsal expanded region of the retinal pigmented epithelium (RPE), but was observed in only in regions where the RPE remained a monolayer.
Expression was primarily in a superficial layer of cells. The mutant expression domain expanded rostrally into what had been prosomere 1, but the gain of expression indicates a shift in the identity of the p1 region to that of mesencephalon.
Expression was detected throughout the superior colliculus. The strongest signal was obtained in the outer dorsal edge of the intermediate zone. Signal was also noted in the superior colliculus ventricular zone.
Expression was detected throughout the superior colliculus. The strongest signal was obtained in the outer dorsal edge of the intermediate zone. Signal was also noted in the superior colliculus ventricular zone.
Expression was detected throughout the superior colliculus. The strongest signal was obtained in the outer dorsal edge of the intermediate zone. Signal was also noted in the superior colliculus ventricular zone.
Expression was observed in ventral longitudinal stripes which extend along the hindbrain, midbrain, and continue into the ventral forebrain. The longitudinal stripes branch off to a region surrounding the zone limitans intrathalamica.
Expression was observed in ventral longitudinal stripes which extend along the hindbrain, midbrain, and continue into the ventral forebrain. The longitudinal stripes branch off to a region surrounding the zone limitans intrathalamica.
Expression was detected in a portion of the ventrocaudal midbrain. Also, weak expression was noted in the pretectum with a sharp posterior limit but a gradual anterior limit near the dorsal thalamus.
Expression was detected in the pretectum. Essentially all the cells were positive, at least at the ependymal layer level. The border of positive cells at the pretectum/midbrain boundary was sharp but the rostral end of the pretectum gradually decreased.
Expression was detected in the basal plate of the mesencephalic tegmentum and in the ventral aspect of the superior and inferior colliculus. There was a gap between the expression domains in the anterior and posterior pretectum.
Strong expression was in the pretectum. Signals in the precommissural and commissural parts of the pretectum were separated by an expression-free gap. Expression was in the basal plate in the tegmentum, and longitudinally in the superior colliculus.
Expression is strong in the superior and inferior colliculus. A strong stripe of expression extends from the inferior colliculus toward the cerebellum. Expression was also detected in the tegmentum.
Expression was detected in the entire alar midbrain and the tectum. There was less expression in the midbrain basal plate, except for a cluster of cells in the region of the primordium of the substantia nigra.
Slightly lower expression in the dorsal TH domain and in the ventricular zone. The line of cells highly expressing Grb10 dorsally along the entire medial mesodiencephalic dopamine neuronal area was clearly affected.
Slightly lower expression in the dorsal TH domain and in the ventricular zone. The line of cells highly expressing Grb10 dorsally along the entire medial mesodiencephalic dopamine neuronal area was clearly affected.
Slightly lower expression in the dorsal TH domain and in the ventricular zone. The line of cells highly expressing Grb10 dorsally along the entire medial mesodiencephalic dopamine neuronal area was clearly affected.
Slightly lower expression in the dorsal TH domain and in the ventricular zone. The line of cells highly expressing Grb10 dorsally along the entire medial mesodiencephalic dopamine neuronal area was clearly affected.
Slightly lower expression in the dorsal TH domain and in the ventricular zone. The line of cells highly expressing Grb10 dorsally along the entire medial mesodiencephalic dopamine neuronal area was clearly affected.
Expression was weak at the rostral half of the midbrain, but it increases gradentally caudalwards to a maximum just in front of the isthmo-mesencephalic transverse boundary at the expected locus of the m2 mesomere.
Expression showed a decreasing dorsocaudal gradient from the ventricular zone. Signal was sharply absent at a slightly evaginated part of the caudal midbrain neuroepithelium that covers the classic transverse sulcus intraencephalicus posterior.
Expression showed a decreasing dorsocaudal gradient from the ventricular zone. Signal was sharply absent at a slightly evaginated part of the caudal midbrain neuroepithelium that covers the classic transverse sulcus intraencephalicus posterior.
Signal was strong in the inferior colliculus. Signal was detected in the superior colliculus and the mantle of the toral bulges. Signal was not detected in the intercollicular area.
Signal was strong in the inferior colliculus. Signal was detected in the superior colliculus and the mantle of the toral bulges. Signal was not detected in the intercollicular area.
Signal was strong in the inferior colliculus. Signal was detected in the superior colliculus and the mantle of the toral bulges. Signal was not detected in the intercollicular area.
Signal was strong in the inferior colliculus. Signal was detected in the superior colliculus and the mantle of the toral bulges. Signal was not detected in the intercollicular area.
Expression was primarily restricted to the dorsal midline. Expression in the ventral midbrain largely mirrors that of Lmx1a in both the progenitors in the ventricular and intermediate zone and in the mantle zone postmitotic cells.
Expression is detected in the ventral domain of all rhombomeres, except rhombomere 1. It was strongest in r2 and r4, corresponding to the precursors of the trigeminal and facial motoneurons.
Expression is detected in the ventral domain of all rhombomeres, except rhombomere 1. It was strongest in r2 and r4, corresponding to the precursors of the trigeminal and facial motoneurons.
Expression was restricted along the antero-posterior axis to prospective rhombomere3 and to a more posterior domain consisting of only a few cells in the lateral neural plate corresponding to prospective rhombomere 5.
The stripes of expression in prospective rhombomeres 3 and 5 broadened and formed sharp boundaries. The domains coincided with Egr2 (Krox-20 lacZ staining). Expression was downregulated in rhombomere 4, but persisted at low level in 2 and dorsally in 6.
At the 6-8 somite stage, no expression was observed in rhombomere 3 and 5. However, low levels of expression were detected in rhombomeres 2, 4 and 6 as in wild type.
At the 6-8 somite stage, no expression was observed in rhombomere 3 and 5. However, low levels of expression were detected in rhombomeres 2, 4 and 6 as in wild type.
Expression was maintained in rhombomere 2 and 4. At 12 somites, rhombomere 3 has almost disappeared in the mutant embryos. Rhombomere 5 remained negative, and a low signal appeared in rhombomere 6.
The caudal stripe of expression extended beyond the r5/r6 boundary to the middle of r6. Expression was also detected in patches of cells detected throughout the presumptive r6 territory.
Faint expression was detected in the epithelium and strong expression in the mesenchyme of the facial dermis region. Expression was faint in the whisker follicle epithelium, but strong in the surrounding mesenchyme.
Expression was detected in a select group of endodermal cells surrounding the ventral wall of the anterior foregut. There was no staining in the dorsal wall of the anterior foregut.
Expression appeared more homogeneous throughout the aortic wall, apparently as much expressed in the aberrant vascular ring as it was in the rest of the aortic media, but was more prominent in the latter.
The aortic B-segment between the dotted lines was clearly aberrant in showing optically lower staining levels caused by reduced cellular volume of smooth muscle cells and increased elastic matrix.
Expression at the B-segment was much lower. Expression in a small number of cells in the B-segment was absent. The relative cellular volume/ matrix ratio was much smaller than elsewhere.
Expression was detected in preameloblast/ameloblast cells and signal accumulated at the basal and apical pole of the cell membrane. The intensity of the signal decreased at the tip of the dental cusps and in cervical loop direction.
Expression was detected in preameloblast/ameloblast cells and signal accumulated at the basal and apical pole of the cell membrane. The intensity of the signal decreased at the tip of the dental cusps and in cervical loop direction.
Expression was detected in preameloblast/ameloblast cells and signal accumulated at the basal and apical pole of the cell membrane. The intensity of the signal decreased at the tip of the dental cusps and in cervical loop direction.
Expression was detected in the apical zone of the inner enamel epithelium. It was not detected in the middle and basal zones of the inner enamel epithelium or the outer enamel epithelium.
Expression was detected in the apical zone of the inner enamel epithelium. It was not detected in the middle and basal zones of the inner enamel epithelium or the outer enamel epithelium.
Expression was strong in the apical, middle and basal zones of the inner enamel epithelium. Expression in the outer enamel epithelium was stronger on the buccal side than on the lingual side.
Expression was strong in the apical, middle and basal zones of the inner enamel epithelium. Expression in the outer enamel epithelium was stronger on the buccal side than on the lingual side.
Expression was detected in the apical, middle and basal zones of the inner enamel epithelium and in the outer enamel epithelium but was stronger in the outer enamel epithelium.
Expression was strong in the outer enamel epithelium and the apical and basal zones of the inner enamel epithelium. The middle zone of the inner enamel epithelium exhibited moderate staining.
Expression was detected in the apical and middle zones of the inner enamel epithelium and in the outer enamel epithelium. Expression was strong in the basal zone of the inner enamel epithelium.
Expression was detected in the apical, middle and basal zones of the inner enamel epithelium and in the outer enamel epithelium but was stronger in the outer enamel epithelium.
Expression was detected in the mesenchyme that surrounds both pancreatic anlagen and the gut. The expression pattern was a dorso-ventral gradient, with the highest levels in the dorsolateral and the lowest in the ventral mesenchyme.
Expression was detected in the starburst amacrine cell processes, which form a meshwork with small holes of relatively constant size. The processes form a dense plexus in two inner plexiform sublaminae.
Expression was detected in the starburst amacrine cell processes, but the spacing in the mutant plexus shows larger holes of variable sizes and abnormal aggregations corresponding to the defects in the soma array.
Expression was reduced in the region of the inner plexiform layer occupied by the axon terminal of type 3 cells. Expression was unaffected in the regions of the axon termini of type 5 and rod bipolar cells.
Immunolabeled axon terminals in the mutant spanned both sublamina 1 and 2 (located above the outermost Calb2-labeled band). The expansion of label was observed across the entire retina. Hcn4-labeled axon terminals were not observed in sublaminae 3-5.
A few Myod1+ cells were observed in the first branchial arch core of mutants at E10.5, but their total number and the fraction of Pitx2(beta-gal)+ cells that was Myod1+ was dramatically reduced.
Expressed intensely in the oral ectoderm where it had begun to invaginate into the mesenchyme. Expressed in the inner enamel epithelium. There was a gradient of decreasing intensity from the oral ectoderm to the inner enamel epithelium.
Expression was detected in chondrocytes, weakly in resting and proliferating chondrocytes, but progressively stronger as chondrocytes matured. No expression was seen near or at articular surfaces in cartilage or perichondrium.
Expression was detected in the periosteum and in cartilage within the growth plate where it was upregulated as chondrocytes mature, but decreased markedly in terminal hypertrophic chondrocytes. Expression was high in developing trabecular bone.
Axonal projection of the ophthalmic branch of the trigeminal nerve was aberrant. The ascending axons of the glossopharyngeal nerve (IX) were fused with those of the vagus nerve (X). Projections from the mesencephalic neurons were not fasciculated.
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 5D). There was also a decrease (about 50%) on the innervation density of the gland compared to PlexinA4 KO.
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 5D). There was also a decrease (about 50%) on the innervation density of the gland compared to PlexinA4 KO.
Levels of Fgf5 mRNA appeared to be relatively high throughout the embryonic ectoderm, with evidence of differential expression along the proximodistal axis; the signal intensity in the most proximal region was lower than in the most distal region.
Expression was detected in a strip of ectoderm underlying the telencephalon. It extends rostrally from a region basal to the optic stalk and ends near the apex of the frontonasal prominence.
Expression was detected in a strip of ectoderm underlying the telencephalon. It extends rostrally from a region basal to the optic stalk and ends near the apex of the frontonasal prominence.
Expression was detected in a strip of ectoderm underlying the telencephalon. It extends rostrally from a region basal to the optic stalk and ends near the apex of the frontonasal prominence.
Expression was detected in a strip of ectoderm underlying the telencephalon. It extends rostrally from a region basal to the optic stalk and ends near the apex of the frontonasal prominence.
Expression was detected in a strip of ectoderm underlying the telencephalon. It extends rostrally from a region basal to the optic stalk and ends near the apex of the frontonasal prominence.
Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm. All cells that were immunopositive were also positive for the expression of MyoD (see Assay MGI:3611794).
Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm. Only a subset of cells that were immunopositive were also positive for the expression of Myogenin (see Assay MGI:3611793).
Expression appeared normal in the mutant. Expression was strong in the ventrally expanded apical ectoderm ridge and ectopic apical ectodermal ridge on the ventral side of the mutant limb.
Authors report that expression was only modestly altered from the wild type pattern, being perhaps slightly elevated in the posterior and distal edge of the limb relative to wild type.
Expression was detected in a stripe at the isthmus and dorsally it is expressed from the isthmus to part of the dorsal thalamus (p2 diencephalon). A negative region was noted in the anterior diencephalon.
Expression was more anteriorly located in the double heterozygote, but the stripe of expression was nearly unchanged. The negative region in the roof of the anterior diencephalon contained a line of expressing cells.
Expression was detected in an asymmetrical pattern enriched towards the anterior mesenchyme compared to the posterior. Comparison to Mki67 staining indicates that Lhx9 is expressed in many proliferative cells.
Expressed in the distal mesenchyme and in regions of condensing cartilage, being more abundant in the posterior domains. An anterior expression domain was also observed at the base of the limb buds.
Expression was detected in spermatogonia and Sertoli cells located adjacent to the basement membrane of the seminiferous tubules and in Leydig cells. No differentiating spermatocytes or spermatids hybridized with the probe.
Expression was detected in the thoracic body wall adjacent to labeling in the limb overlying the heart but not within more dorsal or ventral regions. Expression in the body wall did not extend continuously between the limbs.
Expression was detected in the thoracic body wall adjacent to labeling in the limb overlying the heart but not within more dorsal or ventral regions. Expression in the body wall did not extend continuously between the limbs.
Expression was detected in the thoracic body wall adjacent to labeling in the limb overlying the heart but not within more dorsal or ventral regions. Expression in the body wall did not extend continuously between the limbs.
A small population of Pax3-positive cells was found at the border to the limb bud, but none had entered the limb bud. Some of this population was also Casp3-positive.
Expression was detected in epithelial and equatorial cells. Strong expression was detected in cells of the germinative and early transitional zones with expression decreasing as cells undergo terminal differentiation.
Expression was detected in epithelial and equatorial cells. Strong expression was detected in cells of the germinative and early transitional zones with expression decreasing as cells undergo terminal differentiation.
Expression was in a transient layer in the upper aspect of the internal granule cell layer, as well as in scattered cells in deeper layers of the internal granule cells layer.
A continuous tract of labelled tissues was seen to extend posteriorly from the base of the thyroid duct to below, and around, the aortic arch. These tissues were tentatively identified as the thryoid and thymus glands.
The proportion of Nkx2-1-positive cells labelled by BrdU was similar as in the wild-type (see Fig 7A). But the number of Nkx2-1 positive progenitor cells was significantly lower (see Fig 7B).
Expression formed patches at the base of the hair cells and at the base of the hair cells and in the thin neurites growing almost to the apex of the sensory cells. Weak staining was present in most sensory cells.
Expression was detected in numerous afferent calyces and patches localized at the base of the sensory cells. Staining was intense in the upper part of the calyces. Moderate to intense staining was noted in the hair cell cytoplasms.
Expression was detected in numerous afferent calyces and patches localized at the base of the sensory cells. Staining was intense in the upper part of the calyces. Weak staining was noted in the hair cell cytoplasms.
Expression was strong in all cortical microvessels and in some of the lateral striatal vessels and dorsal thalamic nuclei, but not in ventral thalamic and hypothalamic vessels. Expression was high at the tips of the sprouts and branching points.
