Expression was detected in the mesenchyme of the conotruncal ridges. In the conotruncus, the repartition of positive cells seems regular, with possibly a higher proportion in the distal part.
In an abnormal region of the neural tube, where the neural tube was open, no expression was detected in presumptive roof plate cells, expected to be located at the lateral margins of the open neural tube.
The authors state that the mutants display a dynamic expression pattern, in some embryos it was strong in presomitic mesoderm, others weak in prospective somite and tail bud. One mutant had tail bud only.
Expression was similar to that described for Hox-1.4 (see assay MGI:1344203): Transcripts were generally not present in tissues that formed the floor of the pharynx. Transcripts were not detectable anterior to the thyroid duct.
Expression was restricted to the germinal layer. No expression in the migratory stream, while the well-defined patch of expression in the germinal layers appeared wider and more intensely labeled than in the wild-type.
Expression was found only in ameloblasts along the major buccal cusp of the first mandibular molar. No signal was found in ameloblasts along the contralateral surface. Ameloblasts from the adjacent enamel-free zone have lower, but detectable levels of expression.
Strong immunolabelling is visible in ameloblasts at the cusp tip, in the cervical loop, and in the outer enamel epithelium, but is absent in ameloblasts that have not reached the secretory stage. Immunolabeling was also evident in ameloblasts in general.
Strong immunolabelling is visible in ameloblasts at the cusp tip, in the cervical loop, and in the outer enamel epithelium, but is absent in ameloblasts that have not reached the secretory stage. Immunolabeling was also evident in ameloblasts in general.
Strong immunolabelling is visible in ameloblasts at the cusp tip, in the cervical loop, and in the outer enamel epithelium, but is absent in ameloblasts that have not reached the secretory stage. Immunolabeling was also evident in ameloblasts in general.
Expression was detected in superior cervical ganglion axons that extend cranially along the primitive internal carotid artery. No sympathetic axon projections were found along either nascent external carotid arteries or the third arch arteries.
Like wild type, expression was detected in the distal portion of the urethral plate epithelium (UPE). Unlike wild type, expression was not detected in the proximal UPE and was low or absent in the lateral shelf mesenchyme.
Unlike wild type, expression was strong in the mesenchyme flanking the medial and proximal urethral plate epithelium (UPE) and weak in the mesenchyme flanking the distal UPE. Expression was detected in a narrow band in the genital tubercle mesenchyme.
Expression was strong in the mesenchyme adjacent to the distal urethral plate epithelium (UPE), the UPE and the sinus epithelium. Expression was present along the rostral-caudal axis of the genital tubercle.
Originally this result was annotated to a female-specific term, but as the specimen was not sexed, it is likely the data annotator intended to use a non-gender specific term.
Originally this result was annotated to a male-specific term, but as the specimen was not sexed, it is likely the data annotator intended to use a non-gender specific term.
Originally this result was annotated to a male-specific term, but as the specimen was not sexed, it is likely the data annotator intended to use a non-gender specific term.
Expression is present in the lateral edges on either side of the base of the genital tubercle. This expression does not show sexual dimorphism. (The sex of this specimen was not reported.)
Co-localized staining of R-loop (transcription-replication conflicts, recognized by the S9.6 antibody) was shown. The proportion of R-loop positive cells was significantly increased compared with the wild-type (quantified in Fig 6B).
Co-localized staining of R-loop (transcription-replication conflicts, recognized by the S9.6 antibody) was shown. The proportion of R-loop positive cells was significantly increased compared with the wild-type (quantified in Fig 6B).
Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing hip, tibia and fibula.
There was no expression in the epithelial layer of the branchial arch or in migratory neural crest cells. Transcripts were concentrated in the neural-crest-derived mesenchyme just underlying the most exterior portion of the branchial arches.
In pharyngeal arch 2, stapes condensation was demarcated by Sox9 expression in mesenchyme dorsal to the pharyngeal endoderm. At E11.5, expression was downregulated in the center of the stapes condensation region.
In pharyngeal arch 2, stapes condensation was demarcated by Sox9 expression in mesenchyme dorsal to the pharyngeal endoderm. At E11.5, expression was downregulated in the center of the stapes condensation region.
Expression was in the cytoplasm and staining was punctate. Expression was predominately in the interior face of the rhombic lip, while cells of the exterior face of the rhombic lip were negative. The reporter co-localized with antibody staining.
Expression was in the cytoplasm and staining was punctate. Expression was predominately in the interior face of the rhombic lip, while cells of the exterior face of the rhombic lip were negative. The reporter co-localized with antibody staining.
Expression was localized primarily to the interior face of the rhombic lip. Expression was largely non-overlapping with Atoh1, except for a few cells. Expression ends abruptly at the presumed dorsal border of the rhombic lip.
Expression was detected in the exterior face of the rhombic lip, continuous with the forming external granule cell layer. Expression was largely non-overlapping with Wls with only a few co-expressing cells.
LacZ reporter was detected in the rhombic lip. Co-expression with Wls was seen in the interior face of the rhombic lip in many more cells in the mutant compared to the wild type.
Expression was detected in the rhombic lip as in wild type. Co-expression with Atoh1-reporter was seen in a higher number of cells in the interior face of the rhombic lip in the mutant compared to wild type.
Expression was robust in the interior face of the rhombic lip and light in the exterior face, and strong in the distal part of the rhombic lip. Co-expression was noted with light expressing Pax6-positive cells in the interior face.
Strong expression was detected in the exterior face of the rhombic lip and light expression in the interior face. Co-expression with Wls was noted in the interior face of the rhombic lip.
Expression was strong in a subset of cells localized to the interface between the interior and exterior faces of the rhombic lip. These cells are negative for Wls expression.
Weak expression was detected in the interior face of the rhombic lip and co-expressed Wls. Strong expression was detected at the interface of the interior and exterior faces of the rhombic lip, and these cells did not co-express Wls.
Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing shoulder, radius and ulna.
Expression on the anterior left side of the node was lost, while expression was activated in the midline cells anterior to the node, resulting in a question mark-like expression pattern.
Intense expression was noted in the actively dividing equatorial epithelial cells. The differentiating and elongating cells showed decreased levels. Expression was specifically on the basolateral side rather than the apical side.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression was detected in spermatogonia and Sertoli cells located adjacent to the basement membrane of the seminiferous tubules and in Leydig cells. No differentiating spermatocytes or spermatids hybridized with the probe.
Kash5 staining was observed at telomeric sites in mutant spermatocytes. However, in approximately half of the zygotene-like cells, Kash5 foci remained clustered indicating an arrest at the so-called 'bouquet' stage.
Expression was detected in the perineurium of the dental nerve, as well as the connective tissue surrounding the submaxillary acini, pancreatic acini, and the Langerhans islands of the pancreas.
Expression increased as blastocysts developed. Expression in the inner cell mass (ICM) was heterogenous. Embryos were stained for Nanog and Gata6 to identify the epiblast (Nanog+), extraembryonic primitive endoderm (Gata6+), double positive, and double negative cell populations.
Expression increased as blastocysts developed. Expression in the inner cell mass (ICM) was heterogenous. Embryos were stained for Nanog and Gata6 to identify the epiblast (Nanog+), extraembryonic primitive endoderm (Gata6+), double positive, and double negative cell populations.
Expression increased as blastocysts developed. Expression in the inner cell mass (ICM) was heterogenous. Embryos were stained for Nanog and Gata6 to identify the epiblast (Nanog+), extraembryonic primitive endoderm (Gata6+), double positive, and double negative cell populations.
Expression increased as blastocysts developed. Expression in the inner cell mass (ICM) was heterogenous. Embryos were stained for Nanog and Gata6 to identify the epiblast (Nanog+), extraembryonic primitive endoderm (Gata6+), double positive, and double negative cell populations.
Expression increased as blastocysts developed. Expression in the inner cell mass (ICM) was heterogenous. Embryos were stained for Nanog and Gata6 to identify the epiblast (Nanog+), extraembryonic primitive endoderm (Gata6+), double positive, and double negative cell populations.
Expression increased as blastocysts developed. Expression in the inner cell mass (ICM) was heterogenous. Embryos were stained for Nanog and Gata6 to identify the epiblast (Nanog+), extraembryonic primitive endoderm (Gata6+), double positive, and double negative cell populations.
Expression increased as blastocysts developed. Expression in the inner cell mass (ICM) was heterogenous. Embryos were stained for Nanog and Gata6 to identify the epiblast (Nanog+), extraembryonic primitive endoderm (Gata6+), double positive, and double negative cell populations.
Expression increased as blastocysts developed. Expression in the inner cell mass (ICM) was heterogenous. Embryos were stained for Nanog and Gata6 to identify the epiblast (Nanog+), extraembryonic primitive endoderm (Gata6+), double positive, and double negative cell populations.
Expression increased as blastocysts developed. Expression in the inner cell mass (ICM) was heterogenous. Embryos were stained for Nanog and Gata6 to identify the epiblast (Nanog+), extraembryonic primitive endoderm (Gata6+), double positive, and double negative cell populations.
Expression was restricted to the medial and lateral extremes of the dermamyotome, with prominent expression seen in the epithelial balls of the lateral myotome and overlying dermis of the interlimb region.
Authors state that Pax3 expression patterns (in the future dermomyotome) revealed that paraxial mesenchyme in the thoracic region becomes properly segmented. However, in dorsal somitic regions, an abnormal, flame-like pattern of expression was observed.
Expression was strong within newly forming epithelial somites, but only in the rostral half. Restriction of expression to the rostral half somites was clearest in the two most recently formed somites and was less detectable in more rostral somites.
Expression was detected in all somites except the two to three caudal pairs that were the most recent to segregate. Expression was restricted to the most dorsal part of the somites.
Expression was detected in all somites except the two to three caudal pairs that were the most recent to segregate. Expression was restricted to the most dorsal part of the somites.
Expression was detected in all somites except the two to three caudal pairs that were the most recent to segregate. Expression was restricted to the most dorsal part of the somites. Expression detected in the otic vesicle.
Expression was panretinal, but the level was higher in the vitreal outer neuroblastic layer of the central retina, the extreme scleral outer neuroblastic layer and in the scleral inner neuroblastic layer.
Expression was detected in a small number of cells in the ventricular zone, but they did not overlap with BrdU positive cells. Expression was detected in a large number cells in the ganglion cell layer.
Expression was detected in a stripe in the retina. Expression seems to straddle the ventral border of immunostaining for Aldh1a1 (Ahd2). Single labeled cells barely reach the vitreal margin.
Expression was detected in a stripe slightly dorsal to the optic disc and ventral to immunostaining for Aldh1a1 (Adh2). Selected cell labeling was restricted to the outer retina containing proliferating cells but absent from the inner region.
Expression was detected in the ganglion cells layer. In the neuroblast layer, expression became very low. The majority of Math5-positive cells were negative for Oc2, although a few Oc2-positive/Math5-negative cells could be observed.
Co-expression with Isl1 in the ganglion cell layer continued at E14.5. Very little overlap in expression was observed in the neuroblast layer due to the low levels of Oc2.
Expression was detected in a narrow band in the inner most regions of the retina as in wild type. There was no change in the level or pattern of expression in the mutant.
Expression was detected in a narrow band in the inner most regions of the retina as in wild type. There was no change in the level or pattern of expression in the mutant.
Immunostaining showed a classical focal distribution. The number of cells with foci, as well as the number of foci per cell, was higher in the mutant compared to wild type.
No difference in the immunostaining distribution was observed in the mutant retina compared to wild type. A higher number of ectopic immunopositive cells were observed in the mutant retina.
Expression was detected in neuroepithelial cells that appear to migrate from the basal layer to vitreal regions of the retina. Expression was also observed in the optic fiber layer.
Expression was detected in neuroepithelial cells that appear to migrate from the basal layer to vitreal regions of the retina. Immunoreactivity was not observed in the optic fiber layer.
Expression was decreased in the retina of mutant mice. Both the total number and the percentage of M opsin-positive cones in total cones (PNA-positive) were decreased significantly in the superior retina, by 49% and 26%, respectively.
Expression was detected in proliferating cells. In the dorsal peripheral zone the number of cells exiting cycle (% EdU+ Ki67-/EdU+) in the dorsal ciliary margin zone and peripheral neural retina was comparable to that in the dorsal central zone.
Expression was detected in all layers of the cortical neuroepithelium, from the ventricular zone to the cortical plate. In interphase cells within the ventricular zone, immunostaining appeared to be symmetrically distributed.
Expression was detected in the dorsal region. The deep cortical plate layer was more densely stained than the middle. There was a high rostrolateral-low caudomedial gradation of expression but it was broadly distributed throughout the neocortex.
Expression was detected in the dorsal region. The deep cortical plate layer was more densely stained than the middle. There was a high rostrolateral-low caudomedial gradation of expression but it was broadly distributed throughout the neocortex.
Expression was detected in the dorsal region. There was a high rostrolateral-low caudomedial gradation in the deep cortical plate, but there was weak signal in the superficial layer restricted to the more rostrodorsal neocortex.
Expression was detected in the dorsal region. There was a high rostrolateral-low caudomedial gradation in the deep cortical plate, but there was weak signal in the superficial layer restricted to the more rostrodorsal neocortex.
Expression was detected in an intensely labeled narrow band at the bottom of the cortical plate, and a few cells were abnormally scattered all over the thin cortical plate.
Expression was detected in an intensely labeled narrow band at the bottom of the cortical plate, and a few cells were abnormally scattered all over the thin cortical plate.
Expression was uniform in the upper cortical plate. A number of neurons in the lower cortical plate were also labeled; many of these neurons coexpressed Cux1 (indicated by arrows in image). Arrowheads indicate neurons that do no coexpress Cux1.
Expression was strong in the upper half of the upper cortical plate, and only weak or no expression was observed in the lower half of the upper cortical plate.
Expression was detected in the entire upper cortical plate population. Neurons in the lower cortical plate were also labeled. Arrows indicate neurons that coexpress Pou3f2 and Cux1. Arrowheads indicate neurons that express Pou3f2 but do not express Cux1.
Expression was detected during endochondral ossification of vertebra. Expression was in chondrocytes in the zone of proliferation, zone of hypertrophy, zone of calcification and cartilage breakdown, and in osteoblasts in the zone of ossification.
A high level of expression was detected from the proliferative to the early hypertrophic zone of cartilage. The mRNA expression was completely abolished in the late hypertrophic and calcified zone.
Expression was high in osteoblasts and associated periosteal cells, and along the diaphysis forming the cortex of the long bone after ossification has begun. Expression was low or undetectable in chondrocytes and other cells surrounding osteogenic cells.
Expression was detected in the proximal region of the ganglion, the intraganglionic spiral bundle and the central ganglionic processes. In the neurons, staining was primarily in the perikaryon and the nucleolus.
Shown is expression in cells in a cervical region. There is exclusive expression closer to the dorsal surface, other cells also expressed Dct. Higher magnification was shown in the inset.
The ventral boundary of expression lies in the anterior part next to the limit between the medulla and the pons. Expression was present in the grey matter and absent in the white matter.
Immunopositive neurons were present in the mutant, but they were significantly fewer than in the wild type, with a much weaker density and a diffuse, unstructured distribution of signal. This was particularly prominent for the double labeled neurons.
Immunopositive neurons were present in the mutant, but they were significantly fewer than in the wild type, with a much weaker density and a diffuse, unstructured distribution of signal. This was particularly prominent for the double labeled neurons.
The number of Sst/Pax2 coexpressing neurons was severely reduced in the mutant pre-Botzinger complex. However, the overall number of Pax2-positive neurons in the same region was only marginally affected.
Expression was detected in the mesodermal layer of the yolk sac. In blood islands, the expression was restricted to the peripheral cell layer. In contrast, hematopoietic cells in the center of the blood islands were not labeled.
Expression was low and more posterior (g) or mispositioned to the limb-bud apex (h). Shh levels were much lower or not detectable in most other Xt;ld mutants and could not be reliably detected before E10.25.
Expression was low and more posterior (g) or mispositioned to the limb-bud apex (h). Shh levels were much lower or not detectable in most other Xt;ld mutants and could not be reliably detected before E10.25
