Expression is at the distal edge and in an arc orthogonal to the proximal-distal axis that was broad at the anterior edge and narrowed towards the center of the limb and in a central region.
Expression was detected along the entire proximal margin of the forelimb bud. Expressing cells extended along both the anterior and posterior margins, and labeling was most intense in the zone of polarizing activity (bracket).
Expression was detected along the entire proximal margin of the forelimb bud. Expressing cells extended along both the anterior and posterior margins, and labeling was most intense in the zone of polarizing activity (bracket).
Expression was detected approximately 100 um layer of mesenchyme underneath the ectoderm in all regions of the limb bud. Expression co-localized with proliferation (BrdU staining). Chondrogenic differentiation (Sox9 immunostaining) was mutually exclusive with proliferation and reporter expression.
Expression was detected approximately 100 um layer of mesenchyme underneath the ectoderm in all regions of the limb bud. Expression co-localized with proliferation (BrdU staining). Chondrogenic differentiation (Sox9 immunostaining) was mutually exclusive with proliferation and reporter expression.
Expression was detected along the dorsal and ventral sides of the forelimb buds. Both the dorsal and ventral domains extended toward the proximal aspect of the limbs, but did not reach their distal extremities.
Expression was detected along the dorsal and ventral sides of the forelimb buds. Both the dorsal and ventral domains extended toward the proximal aspect of the limbs, but did not reach their distal extremities.
In the forelimb bud, the highest level of staining was observed around a distal rim and gradually decreased into the more proximal tissues, suggesting that the expression increases along the proximo-distal axis.
In the forelimb bud, the highest level of staining was observed around a distal rim and gradually decreased into the more proximal tissues, suggesting that the expression increases along the proximo-distal axis.
No expression in mature collecting ducts. Staining for a marker of collecting tubules, Dolichos biflorus lectin, in contrast, was positive in mutant kidneys in a similar pattern when compared to wild type kidneys.
Weak expression in the spendymal layer of the alar plate, but the strong signal in dorsalmost cells (seen in wild-type) was absent, and there was a dorsalmost region where no expression was detected.
Expression was not detected in the dorsalmost region. In other regions of the presumptive alar plate, expression was significantly stronger than in wild type embryos. The ventralmost region negative for expression appeared to be enlarged.
Expression in the ventricular zone throughout the CNS, although at different levels in different regions. In many regions, expression was not seen in all cells of the ventricular zone.
Expression was detected in the lateral membrane of the inner dental epithelial cells of the labial side, including preameloblast, secretory and mature ameloblasts, and in the undifferentiated epithelial sheet of the lingual side inner dental epithelium.
Expression was detected in the lateral membrane of the inner dental epithelial cells of the labial side, including preameloblast, secretory and mature ameloblasts, and in the undifferentiated epithelial sheet of the lingual side inner dental epithelium.
Ptc in the molars was restricted to the mesenchyme underlying the tooth thickening. In the mandibular and maxillary incisors, the domain of Ptc appeared to have extended more laterally but was still localized in the odontogenic region of the mandible.
Gli1 was expressed in the odontogenic epithelium and mesenchyme of the incisors, whereas in the molars the medial pattern of expression was maintained. Gli1 expression was slightly lower than that of Ptc.
Expressed in the crypts and in the basal and middle region of the villi. The signal was less pronounced in the mature cells close to the tips of the villi.
Expression was detected in the oral mucosa region. No difference was noted between signals from epithelium and mesenchyme. Expression was faint in the tooth germ, but strong in the surrounding mesenchyme.
Msx-2 expression was downregulated in the posterior mesoderm of the developing handplate when compared to wild-type controls. The handplate contains a group of posterior msx-2-expressing cells that are surrounded on both sides by nonexpressing cells.
Expression was found in a similar pattern to E12.5. Staining was also found in distal mesodermal cells, bordering the region where separation of the digits has already taken place.
In the antimesometrial pole, expression was in a cup-shaped region in the secondary decidual zone. In the mesometrial pole, labeled cells appeared to radiate from the mesometrial chamber into neighboring areas.
Expressed in decidual cells adjacent to the ectoplacental cone. In the area of placenta formation, expression was in the decidual tissue neighboring trophoblast giant cells. Expression in this mutant is the same as in wild-type embryos.
In the vast majority of embryos examined there was no expression (or there was weak expression in a few scattered cells in the presumptive anterior visceral endoderm and the definitive endoderm regions).
There was weak expression in a few scattered cells in the presumptive anterior visceral endoderm and the definitive endoderm regions (in the central portion of the epiblast and near the distal tip region). (In many cases there was no expression.)
Crabp1 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
Hoxa3 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
Prx1 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fashion to Pax3.
Prx2 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
c-met was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
There was a considerable decrease in Cux2-positive cells in the mutant compared with wild type. The Cux2-positive population of upper layer neurons was lost from the subventricular zone and underrepresented in the intermediate zone.
There was no expression in the lateral plate of this specimen. In the lateral plate mesoderm of mutant animals, 28% had expression on the left side, 25% on the right side, 31% on both sides, and 16% had no expression.
Expression was elevated markedly in both sides of the lateral plate mesoderm. In 6 out of 10 embryos ectopic expression in the right had expanded posteriorly although it was weaker in intensity compared to the left.
Expression was elevated markedly in both sides of the lateral plate mesoderm. In two thirds of the embryos ectopic expression in the right posterior region showed discrete anterior and posterior expression domains.
Expression was elevated markedly in both sides of the lateral plate mesoderm. In two thirds of the embryos ectopic expression in the right posterior region showed discrete anterior and posterior expression domains.
Expression was elevated in the left lateral plate mesoderm and extended more posteriorly on the left than in wild type. Ectopic expression was detected in the anterior half of the right lateral plate mesoderm.
Intense expression was detected on both sides of the lateral plate mesoderm. In 3 of 7 embryos this expression was continuous, whereas others showed discrete anterior and posterior domains. The right posterior expression was weaker than the left.
In 84% of the embryos the left-sided expression was absent (shown here). In 16% of the embryos a low level of expression was seen in a small region of left lateral plate mesoderm adjacent to the node.
In 84% of the embryos the left-sided expression was absent. In 16% of the embryos a low level of expression was seen in a small region of left lateral plate mesoderm adjacent to the node (shown here).
In 84% of the embryos the left-sided expression was absent. In 16% of the embryos a low level of expression was seen in a small region of left lateral plate mesoderm adjacent to the node (shown here).
Expression appeared to be delayed from the 4 to the 6-somite stage. Expression was weaker, and its laterality was perturbed often bilateral (3 out of 9 embryos). Weak expression on the left in one 6-somite embryo.
Expressed in all wild-type embryos from 2 to 6 somites and was restricted to the left side. Strong expression in 13 out of 15 embryos. Weak expression in 2 embryos.
Expression is in the entire ventricular layer in the dorsal spinal cord, in narrow stripes within the ventricular layer ventral spinal cord; and there was a narrow gap in between.
Expressed in V3 interneuron progenitors in the ventral region, similar as in the wild-type. The increased expression of Foxa2 and Nkx2-2 double positive cells in Cdo-/- mutants was not seen in Boc-/-.
Expression was detected in the medial dorsal thalamic nucleus, the laterodorsal thalamic nucleus, the lateroposterior thalamic nucleus, the ventral posterolateral thalamic nucleus, the central posteromedial thalamic nucleus, and the paraventricular thalamic nucleus.
Expression was detected in the laterodorsal thalamic nucleus, the central posteromedial thalamic nucleus, the central medial thalamic nucleus, the lateroposterior thalamic nucleus, the ventral posterolateral thalamic nucleus, the medial dorsal thalamic nucleus, the paraventricular thalamic nucleus.
Expression was detected in the laterodorsal thalamic nucleus, the central posteromedial thalamic nucleus, the central medial thalamic nucleus, the lateroposterior thalamic nucleus, the ventral posterolateral thalamic nucleus, the medial dorsal thalamic nucleus, the paraventricular thalamic nucleus.
Expression was detected in large cells in the fiber tracts from vermal sections and in the brainstem. As these cells were not observed with the 5'Nav2 probe, they appear to be expressing the short transcript.
Expression was detected in large cells in the fiber tracts from vermal sections and brainstem, as in wild type. As these cells were not observed with the 5'Nav2 probe, they appear to be expressing the short transcript.
Expression was detected in the starburst amacrine cells that had migrated to the appropriate cell layers as in wild type. There were regions where the somata were clumped in the mutant.
Expression was detected in the type 2 bipolar cells. In addition, a subset of Hcn4-positive type 3a cells was weakly labeled. A population of amacrine cells were also positive.
A significant reduction of expression was observed in the type 2 bipolar cells. A subset of weakly labeled Hcn4-positive type 3a cells was smaller in size in the mutant. Expression in the amacrine cells was unchanged in the mutant.
Ectopic Tacr3 expression was observed in Hcn4-immunolabeled cell somas of putative type 3a cells. Expression in type 1 and 2 bipolar cells was not co-labeled with Hcn4. There was an increased labeling of Tacr3 in sublamina 2.
Expression was detected in cells that display chracteristics of both type 2 and 3a bipolar cells. Not all Irx6-bgal-positive cells co-immunolabeled Hcn4. Bhlhe22 immunolabeling is present in some of the Irx6:bgal-positive cells that do not co-immunolabel Hcn4.
Expression was detected in cells that display chracteristics of both type 2 and 3a bipolar cells. Not all Irx6-bgal-positive cells co-immunolabeled Hcn4. Bhlhe22 immunolabeling is present in some of the Irx6:bgal-positive cells that do not co-immunolabel Hcn4.
Expression was detected in endothelial precursors. Phenotypic alterations were detected in the mutant in the endocardial tube of the heart and vascular connections of the anterior cardinal vein. The intersomitic vessels did not form in the mutant.
Staining indicated severe cardiovascular defects - abnormal vascularization, absence of dorsal aorta and intersomitic vasculature, and cardia bifida. No staining was detected on the dorsal side of the head structures.
In E8-8.5 embryos immunoreactivity was associated with many types of F-actin-containing fibrillar structures. Staining was frequently discontinuous and was concentrated in broad bands that often coincided with decreases or discontinuities in F-actin staining.
In E8-8.5 embryos immunoreactivity was associated with many types of F-actin-containing fibrillar structures. Staining was frequently discontinuous and was concentrated in broad bands that often coincided with decreases or discontinuities in F-actin staining.
In E8-8.5 embryos immunoreactivity was associated with many types of F-actin-containing fibrillar structures. Staining was frequently discontinuous and was concentrated in broad bands that often coincided with decreases or discontinuities in F-actin staining.
In E8-8.5 embryos immunoreactivity was associated with many types of F-actin-containing fibrillar structures. Staining was frequently discontinuous and was concentrated in broad bands that often coincided with decreases or discontinuities in F-actin staining.
The size of the hypertrophic chondrocytes zone relative to the total size of the cartilage was reduced in this mutant compared to wild type as well the size of the individual cells.
A high level of expression was detected from the proliferative to the early hypertrophic zone of cartilage. The mRNA expression was completely abolished in the late hypertrophic and calcified zone.
In the cortex, expression was detected in the nuclei of epithelial cells constructing the convoluted and straight proximal tubules. Immunohistochemistry showed a positive reaction in the neck of the proximal tubule.
In the cortex, expression was detected in the nuclei of epithelial cells constructing the convoluted and straight proximal tubules. In situ hybridization did not detect a signal in the neck of the proximal tubule.
Expression was detected in the migratory wavefront of enteric neural crest-derived cells in the mid-colon. There was variability in the number of Tuj1+ neurites at the wavefront; this sample showed Tuj1+ neurites associated with most of the Sox10+ cells.
Expression was detected in the migratory wavefront of enteric neural crest-derived cells in the mid-colon. In this sample, very few Tubb3 immunopositive neurites were associated with the most caudal Sox10+ cells.
Expression was detected in the cell bodies of differentiating ganglion cells and undifferentiated neural precursors. Slightly stronger expression was detected in the axons of the developing inner plexiform layer.
Expression was observed in both the soma and the axons of retinal ganglion cells. Staining delimited an area adjacent to the vitreal surface of the retina containing most of the retinal ganglion cell bodies.
Expression was primarily detected in the ciliary margin of the neural retina. A few immunopositive cells were seen in the bottom of the ganglion cell layer. Expressing cells were not detected in the neuroblast layer.
The area showing a break in the presumptive outer limiting membrane correlated with a decrease in Yap1 expression. There was partial fusion when the duplicated retina abutted the original retina.
The area showing a break in the presumptive outer limiting membrane correlated with a decrease in Yap1 expression. There was partial fusion when the duplicated retina abutted the original retina.
Strong expression was detected in the future horizontal cell layer. Extensive overlap between Tfap2a and Tfap2b expression was observed (80% of immunopositive cells). In the outer retina, putative horizontal cells exhibiting strong Tfap2b signal were weakly stained for Tfap2a.
Extensive overlap between Tfap2a and Tfap2b expression was observed (80% of immunopositive cells). In the outer retina, putative horizontal cells exhibiting strong Tfap2b signal were weakly stained for Tfap2a.
Expression was detected both singly and co-labeled with Tfap2c. The Tfap2c-positive population was smaller than the Tfap2b-positive population. Only 3.4% of the immunopositive population was double labeled in the mature retina.
Expression was detected both singly and co-labeled with Tfap2b. The Tfap2c-positive population was smaller than the Tfap2b-positive population. Only 3.4% of the immunopositive population was double labeled in the mature retina.
Expression was strong in the basal cell layer and reduced or absent in the granular layer and stratum corneum. Expression was detected in the spinous cell layer and adherens junctions.
Expression was strong in the basal cell layer and reduced or absent in the granular layer and stratum corneum. Expression was detected in the spinous cell layer and adherens junctions. No differences were observed between mutant and wild type staining.
In most skin areas, the basal epidermal layer was of normal thickness and stained less intensely than the rest of the epidermis. In some areas, however, the basal epidermal layer was thinner and was stained intensely.
In the more developmentally advanced regions on the head and thorax, an increase in E2f2 expression was evident. Specifically, expression was detected in the underlying mesenchyme with greater levels in the epithelium.
In the ventral region, which has started to stratify, expression was detected both in the epithelium and, to a lesser extent, in the underlying mesenchyme. In the dorsal regions, where the epithelium hasn't differentiated into layers, expression levels were low.
Expression in the cytocortex was detected in the apical microvillous pole on the outer surface of each blastomere in 57% of the embryos but absent from the nascent apicolateral tight junctions site.
Expression extended over the caudal two thirds of the midbrain. Immunoreactivity was not throughout the thickness of the neuroepithelium, but in single neuroblasts on the pial surface of the midbrain.
Expression in the perichondrium of digits in the double-mutant autopod was similar to that seen in the wild-type, although the double-mutant handplate had a cleft and a duplication of digit 5.
In the forelimb autopod, expression was elevated in the ectoderm over interdigital regions compared to the ectoderm over the digits. Knots of expression were detected in ventral tendon-forming regions.
Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.
Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.
Thick bands of expression radiated from the hand and along the digit borders. Expression was also visible at presumptive joint sites along the digits, but was not observed in the distal digit tips.
Thick bands of expression radiated from the hand and along the digit borders. Expression was also visible at presumptive joint sites along the digits, but was not observed in the distal digit tips.
Expression was detected in the epithelial layer of the fused part of the eyelid, including the epidermis on the outer eyelid surface and the conjunctiva on the inner eyelid surface.
Expression was detected in small clusters of primary myotubes. The clusters were oriented parallel to each other and spanned the muscle from end to end. Sample was foot muscle.
Expression was detected in small clusters of primary myotubes. The clusters were oriented parallel to each other and spanned the muscle from end to end. Sample was foot muscle.
Expression was detected in several neural crest cell populations, including cells within the wall of the midgut that are enteric nervous system precursors, future spinal ganglia, and future sympathetic ganglia surrounding the aorta.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
