Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
The expression domain in the paraxial mesoderm spanned 7 to 8 somites. Expression was robust from somite 14-20, whereas weak staining was observed in the 13th and occasionally in the 21st somite.
Expression was limited to the apical surface of epithelial cells. Only weak expression was in the basal portion of the auditory epithelium. Weak expression was also noted in the organ of Corti.
Expression was only observed in a single column of cells at the medial border of the prosensory domain near the basal cochlea duct where inner hair cells will appear, but not in the apical or medial regions.
Expression was only observed in a single column of cells at the medial border of the prosensory domain near the basal cochlea duct where inner hair cells will appear, but not in the apical or medial regions.
Expression was only observed in a single column of cells at the medial border of the prosensory domain near the basal cochlea duct where inner hair cells will appear, but not in the apical or medial regions.
Positive cells were detected in the midbrain, and in condensations at the normal positions of the fifth and seventh cranial ganglia. The pattern of staining was similar to the 9.5-day wild-type embryo shown in Fig. 3A.
Expression was greatly reduced in double mutants, particularly in the region caudal to the sulcus telodiencephalicus. Caudal limit shifted rostrally and expression was restricted to the more dorsal region.
Expression was strong in a small ventral midline patch around the mesencephalon/metencephalon junction and weak in a thin transverse band of cells at the anterior end of the hindbrain.
Expression is located laterally to the Shh-expressing cells. Expression is largely absent in the Shh-expressing domain. There was some overlap between Shh and Gli1 expression at their domain boundaries (low expression).
Expressed in inner hair cells, Dieters cells, and inner phalangeal cells. Hair cell differentiation appeared relatively unaffected, although the entire saccular structure was shaped differently than in the controls.
Expression was reduced in the paraventricular and supraoptic nuclei of the mutant compared to wild type. Transcript was detected in a domain located midway between the third ventricle and the surface of the brain.
Expression was reduced in the paraventricular and supraoptic nuclei of the mutant compared to wild type. Transcript was detected in a domain located midway between the third ventricle and the surface of the brain.
Expression was reduced in the paraventricular and supraoptic nuclei of the mutant compared to wild type. Transcript was detected in a domain located midway between the third ventricle and the surface of the brain.
Expression was reduced in the paraventricular and supraoptic nuclei of the mutant compared to wild type. Transcript was detected in a domain located midway between the third ventricle and the surface of the brain.
Expression was detected in the arcuate nucleus. Subcellular localization of immunoreactivity was diffuse cytoplasmic staining in neurons. In addition, large cytoplasmic immunoreactive puncta were observed corresponding to stigmoid bodies.
Expression was detected in the arcuate nucleus as in +/+. Subcellular localization of immunoreactivity was diffuse cytoplasmic staining in neurons. Immunoreactive puncta were observed in fewer neurons in +/- embryos compared to +/+ embryos.
Expression was detected in the dorsomedial nucleus as in +/+. Subcellular localization of immunoreactivity was diffuse cytoplasmic staining in neurons. Immunoreactive puncta were observed in fewer neurons in +/- embryos compared to +/+ embryos.
Expression was detected in the dorsomedial nucleus. Subcellular localization of immunoreactivity was diffuse cytoplasmic staining in neurons. In addition, large cytoplasmic immunoreactive puncta were observed corresponding to stigmoid bodies.
Expression was detected in the medial preoptic area and the anterior hypothalamus. Expression was not evenly dispersed, but occurred in waves. No expression was found in the suprachiasmatic nucleus.
Expression was detected in the hair cell layer of the cochlear and vestibular systems and in a few cells in the supporting cell layer. Staining was absent from the stereociliary bodies in the cochlear and vestibular labyrinth.
Expression was detected in a reduced number of hair cells relative to wild type in the sensory epithelium of the cochlear and vestibular systems and a minor fraction of cells within the supporting cell layer of the vestibular system.
Expression was detected in the hair cell layer of the cochlear and vestibular systems and in a minor fraction of cells in the supporting cell layer. Expression was in a reduced number of cells compared to wild type.
Expression was detected in the hair cells of the lumenal layer, staining colocalized with Myo7a. Labeled cells in the supporting cell layer appear to be streaming towards the lumenal layer, staining did not colocalize with Myo7a.
Expression was detected in all somites except the two to three caudal pairs that were the most recent to segregate. Expression was restricted to the most dorsal part of the somites. Expression detected in the otic vesicle.
Staining within the periotic mesenchyme, wich was starting to display a morphology characteristic of mature cartilage. Staining in all cartilages of the inner ear, in particular the tympanic ring surrounding the cochleal and saccular epithelia.
Expression was detected in the hair cells of the lumenal layer. Also, labeled cells are present in the supporting cell layer that appear to be streaming towards the lumenal layer.
Expression was detected in the hair cells of the lumenal layer. Also, labeled cells are present in the supporting cell layer that appear to be streaming towards the lumenal layer.
Expression was restricted to the differentiated hair cells of the lumenal layer in the vestibular sensory epithelia, where expression overlapped with Pou4f3 and was absent from the supporting cell layer.
Expression was restricted to the differentiated hair cells of the lumenal layer in the vestibular sensory epithelia, where expression overlapped with Pou4f3 and was absent from the supporting cell layer.
Expression was most prominent in cochlear duct and the nearby periotic mesenchyme. Only weak expression could be detected in the dorsal vestibular region. Expressed in the tip of the ventrally extending cochlear duct.
Expression extend across the site that would form the utriculosaccular constriction and down the posterior wall of the basal cochlear duct. An unbroken band was present apically, but it weakened basally.
As in the wild type embryo, expression was detected in the mandibular branch of the trigeminal nerve and in the facial nerve. However, there were abnormalities in nerve development in the mutant.
As in the wild type embryo, expression was detected in the mandibular and maxillary branches of the trigeminal nerve and in the facial nerve (which was not shown). However, there were abnormalities in the nerve development in the mutant.
In this mutant nerves extend in the correct direction to their targets but defasciculate and occupy a wider area compared to wild-type embryos. No abnormality was seen in the oculomotor nerve.
In this mutant nerves extend in the correct direction to their targets but defasciculate and occupy a wider area compared to wild-type embryos. No abnormality was seen in the oculomotor nerve.
Sympathetic axons had failed to extend rostrally beyond the point they reached at E13.5. The ectopic nerve emanating from the otic ganglion was seen, which did not fasciculate with the sympathetic fibers.
Expression was detected in a more anterior location up to the level of the 4th prevertebra. In this region was limited to a population in the mediolateral region of the mantle layer.
High expression was maintained in knee articular cartilage at postnatal month 2 of tibia above the tidemark and calcified cartilage. In femoral articular cartilage, expression was dramatically reduced in 2 months old animals.
Strong expression was largely restricted to deep zone columnar chondrocytes above the tidemark and calcified cartilage. Superficial zone cells mostly do not express Hoxa11eGFP. Expression was absent from femoral cartilage.
The superficial zone of the articular cartilage was stained in the ki/+ tissue. Staining was also detected in the pericellular domain surrounding a number of chondrocytes located throughout the entire depth of the articular cartilage.
The superficial zone of the articular cartilage was stained in the ki/ki tissue. Staining was also detected in the pericellular domain surrounding a number of chondrocytes located throughout the entire depth of the articular cartilage.
Expression was detected in differentiated trophoblast cells surrounding the maternal sinuses in the distal end of labyrinth layer and in a subset of trophoblast cells in the proximal region of the labyrinth layer.
There was a mild malalignment between the atrial septum and the ventricular septum. The tricuspid valve overrided the inlet ventricular septal defect and was positioned for its major part above the left ventricle.
Expressed in the central sclerotomes of the mature rostral somites as stripes along the rostral and caudal somite borders. Clearly respected the somite borders. Expression was evenly distributed across the borderlines.
Expression was not obviously up-regulated in the rostral sclerotome halves despite the putative defect of the rostro-caudal polarities in the sclerotomes. No expression along the rostral borders of mature thoracic sclerotomes. Many somite borders already became indistinct.
Expression was not obviously up-regulated in the rostral sclerotome halves despite the putative defect of the rostro-caudal polarities in the sclerotomes. Expression along the rostral borders of the sclerotomes was still weak or not detectable in thoracic sclerotomes.
Expression decreased in the region between the limbs in the mutant compared with wild type. However, expression level was comparable to wild type control embryos caudal to the hindlimb bud.
Expression decreased in the region between the limbs in the mutant compared with wild type. However, expression level was comparable to wild type control embryos caudal to the hindlimb bud.
In the palatoglossus region, expression was abundant in cranial neural crest-derived mesenchymal cells, with the highest expression in the region close to the epithelium. A few labeled myogenic cells were also found.
In the palatoglossus region, expression was abundant in cranial neural crest-derived mesenchymal cells, with the highest expression in the region close to the epithelium. A few labeled myogenic cells were also found.
Crabp1 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
Hoxa3 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
Prx1 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fashion to Pax3.
Prx2 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
c-met was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
In the incisors the immature odontoblasts showed strong expression whereas Opa3 was weakly expressed in mature odontoblasts at E18.5. In contrast, strong expression was observed in ameloblasts in both anterior and posterior regions of incisors at E18.5.
In the incisors the immature odontoblasts showed strong expression whereas Opa3 was weakly expressed in mature odontoblasts at E18.5. In contrast, strong expression was observed in ameloblasts in both anterior and posterior regions of incisors at E18.5.
In the incisors the immature odontoblasts showed strong expression whereas Opa3 was weakly expressed in mature odontoblasts at E18.5. In contrast, strong expression was observed in ameloblasts in both anterior and posterior regions of incisors at E18.5.
In the incisors the immature odontoblasts showed strong expression whereas Opa3 was weakly expressed in mature odontoblasts at E18.5. In contrast, strong expression was observed in ameloblasts in both anterior and posterior regions of incisors at E18.5.
Expression was present at the synapses of photoreceptors, and at lower levels at the outer limiting membrane. Expression was detected in a continuous band at the outer limiting membrane.
Expression was ubiquitous with some enhanced signals in the ventral mesenchyme of the proximal region of the genital tubercle. Some increase in expression in the male compared to female was noted.
Expression was detected in cells in the compact layer of the anterior myocardial wall of the left ventricle. Staining was not seen in the posterior of the left ventricle.
Expression was detected in the anterior and left lateral wall of the left ventricle. In this section, label was seen as a continuous strip of cells toward the top of the ventricle. Label was only in the compact layer.
Expression was detected in the anterior and left lateral wall of the left ventricle. In this section, label was seen as a continuous strip of cells toward the top of the ventricle. Label was only in the compact layer.
Expression was detected at a more posterior level in the bending neural folds and in cells migrating lateral to the neural folds, which appear to be hindbrain neural crest.
Expression was detected in neural crest cells emerging from the rhombomere 5/6 boundary region and migrating ventrally into the third branchial arch as a thin stream clearly separated from the otic vesicle.
Expression was detected in neural crest cells emerging from the rhombomere 5/6 boundary region and migrating ventrally into the third branchial arch as a thin stream clearly separated from the otic vesicle.
Expression was detected in migrating neural crest cells. The trunk neural crest cells are random and disorderly in the mutant. In contrast, the cranial neural crest cells looked comparatively normal.
Rostrally, expression in neural crest cells in somites were aggregating at future dorsal root ganglia and sypathetic ganglia. Caudally, expression was in neural crest cells migrating in orderly streams through the rostral half of the somites.
The frong of migrating enteric neural crest cells was located more anteriorly compared to wild type, just beyond the pancreatic anlage. The total number of stained cells was reduced in the mutant.
Expression was detected in the migratory wavefront of enteric neural crest-derived cells in the mid-colon. There was variability in the number of Tuj1+ neurites at the wavefront; this sample showed Tuj1+ neurites associated with most of the Sox10+ cells.
Expression was detected in the migratory wavefront of enteric neural crest-derived cells in the mid-colon. In this sample, very few Tubb3 immunopositive neurites were associated with the most caudal Sox10+ cells.
Migration pattern was slightly altered in the mutants due to the absence of the otic placode and pharyngeal pouches, but there was no obvious difference in the numbers of cranial neural crest cells entering the pharyngeal region.
Strongly expressed in the cortex, in uninduced mesenchymal cells in the nephrogenic zone and in ureteric bud tips. A weaker signal was detected in mesenchymal cells in the medulla and around the ureter.
Expressed throughout the kidney in mesenchymal cells in both the medullary interstitium and the nephrogenic zone, and at ureteric bud tips, except for condensing mesenchymal structures where expression was downregulated.
Expression was detected in mesenchyme underlying the epithelium of the posterior tongue, some mesenchymal condensates adjacent to the epithelium of the lateral surface of the tongue, and mesenchyme underlying the vallate papilla epithelium.
Expression was detected in clusters of mesenchymal cells that flank the genioglossus muscles and in cell aggregates located at the baso-lateral margins of the mesenchyme of the posterior tongue.
Expression was detected in clusters of mesenchymal cells that flank the genioglossus muscles and in cell aggregates located at the baso-lateral margins of the mesenchyme of the posterior tongue.
Expression was detected in clusters of mesenchymal cells that flank the genioglossus muscles and in cell aggregates located at the baso-lateral margins of the mesenchyme of the posterior tongue.
This mutant has an ovotestes composed of testicular cells and ovarian cells. The ratio of FOXL2-positive cells to SOX9-positive cells was increased in Tet2/Kdm3a-double deficient gonads as compared to Kdm3a-deficient gonads.
This mutant has an ovotestes composed of testicular cells and ovarian cells. The ratio of FOXL2-positive cells to SOX9-positive cells was increased in Tet2/Kdm3a-double deficient gonads as compared to Kdm3a-deficient gonads.
Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing ankle and wrist.
Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing ankle and wrist.
Expression was detected in the proximal part of the limb bud. Some labeled cells were intermingled with Cdh15-positive cells, but no doubly stained cells were observed. Expression was also in the distal region of the hindlimb bud.
Expressed in the chorda-lingual nerve bundle, which enters the tongue base, then grow toward the dorsal epithelium. The nerve endings was restricted to two narrow bands that extended longitudinally on both sides of the midline region.
Expression was detected in the lateral habenula. Co-labeling with Nwd2 was observed in a few scattered neurons among numerous immunstained neurons. No Nwd2-positive;Elavl3/4-negative cells were seen in lateral habenula.
Expression was detected in the lateral habenula. Co-labeling with Nwd2 was observed in a few scattered neurons among numerous immunstained neurons. No Nwd2-positive;Elavl3/4-negative cells were seen in lateral habenula.
Expression was detected in the lateral habenula. Co-labeling with Nwd2 was observed in a few scattered neurons among numerous immunstained neurons. No Nwd2-positive;Elavl3/4-negative cells were seen in lateral habenula.
Expression was detected in the lateral habenula. Co-labeling with Nwd2 was observed in a few scattered neurons among numerous immunstained neurons. No Nwd2-positive;Elavl3/4-negative cells were seen in lateral habenula.
Ptc in the molars was restricted to the mesenchyme underlying the tooth thickening. In the mandibular and maxillary incisors, the domain of Ptc appeared to have extended more laterally but was still localized in the odontogenic region of the mandible.
Gli1 was expressed in the odontogenic epithelium and mesenchyme of the incisors, whereas in the molars the medial pattern of expression was maintained. Gli1 expression was slightly lower than that of Ptc.
Immunopositive fibers extend from the diencephalon toward the ventral surface of the pallidum. The bundle connects the dorsal thalamus and the basal-marginal telencephalon and was not detected in wild type.
In 64% of embryos, expression in presomitic mesoderm and somite detected as 2 distinct stripes. In 2 of 19 embryos examined, expression in S1 (most newly formed somite) was weaker than in S0 (forming somite).
Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.
Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.
