Expressed around the ventricle. Expressed in a band of cells a short distance away from the apical surface of the ventricles. In addition, in cortex there was a second band of cells closer to the ventricle.
Expression was detected in a migratory stream from the caudal ganglionic eminence to the caudal cortex and hippocampus. A majority of the migrating neurons were positive for both Nr2f2 and Calb1 (71% of the Calb1+ cells).
Expression was detected in a migratory stream from the caudal ganglionic eminence to the caudal cortex and hippocampus. A majority of the migrating neurons were positive for both Nr2f2 and Calb1 (71% of the Calb1 cells).
Expression was detected in a high-caudomedial to low-rostrolateral gradient in the telencephalic evagination and was restricted to progenitors. Staining included the primordia of the choroid plexus. No expression was detected in the roofplate.
Expression was detected in a high-caudomedial to low-rostrolateral gradient in the telencephalic evagination and was restricted to progenitors. Staining included the primordia of the choroid plexus. No expression was detected in the roofplate.
Apoptosis (i.e., cleaved caspase-3+ cells) was rare in the cortex, hippocampal hem (future hippocampus) and basal ganglia. The majority of cells undergoing apoptosis in the cortex and basal ganglia also stained positive for H2ax.
Expression was scarce in the miR-9-2/3 double mutant pallium, but apparently normal in the miR-9-2/3 double mutant subpallium, suggesting that interneurons were generated, but their migration was impaired in the miR-9-2/3 double mutants.
Expression was scarce in the miR-9-2/3 double mutant pallium, but apparently normal in the miR-9-2/3 double mutant subpallium, suggesting that interneurons were generated, but their migration was impaired in the miR-9-2/3 double mutants.
Expressed in a domain converging toward the midline with ectopic cells near the midline in the mutant. The mutant rostral corridor was wider and less compact. The caudal extension of the corridor is severely reduced or completely lacking.
Expression was detected in the perichondrium and surrounding tissues of the radius and ulna with increased intensity in the area surrounding the center of the cartilage template where blood vessels will penetrate into the cartilage.
Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing shoulder, radius and ulna.
An average of 17% of labeled endothelial cells are found, usually prismatic or cubic. Staining density in mesenchymal cells in the proximal part was higher than in the distal part.
Expression was present in the ossification zone of the skull base and within and around the bony trabeculae of the basioccipital bone, but its level is much reduced in the mutant relative to wild type.
Expression was present in the periosteal bone collar, the osteogenic front, and the trabecular bone of the skull base, but its level is decreased in the mutant relative to wild type.
Expressed in the inner enamel epithelium, the precursors of enamel producing ameloblasts, but it was also detected, at somewhat lower levels, in the outer enamel epithelium and in stellate reticulum in both first and second molars.
Expressed in the inner enamel epithelium, the precursors of enamel producing ameloblasts, but it was also detected, at somewhat lower levels, in the outer enamel epithelium and in stellate reticulum in both first and second molars.
Expression was exclusively in neural cell bodies; no other cell types were stained. Expression was in many regions of the limbic system: septum nucleus, horizontal & vertical limbs of the Diagonal band, hippocampus, amygdaloid nucleus, & habenula nucleus.
Expression was detected in the rostral hippocampal formation. Both the level of expression and pattern of label change with time in the dentate gyrus and pyramidal neurons of Ammon's horn.
Expression was detected in the rostral hippocampal formation. Both the level of expression and pattern of label change with time in the dentate gyrus and pyramidal neurons of Ammon's horn.
Beta-gal staining was higher in the -/- compared to +/-, and the staining pattern differed. Authors indicate the difference between +/- and -/- was due solely to the lacZ allele dosage.
Beta-gal staining was higher in the -/- compared to +/-, and the staining pattern differed. Authors indicate the difference between +/- and -/- was due solely to the lacZ allele dosage.
Expression levels are higher in testis than ovary. In the testis prominent expression was evident in the testis cords with background levels of expression observed in the adjoining mesonephric tissue.
Expression was detected in the muscle fibers and the sarcolemma as well as around the muscle fiber nuclei in a striped pattern. Expression pattern was similar to that of alpha-actinin, but was not merged.
Expression was detected in the muscle fibers and the sarcolemma as well as around the muscle fiber nuclei in a striped pattern. Expression pattern was similar to that of alpha-actinin, but was not merged.
Expression was detected in the autopod region. There was a slight expansion in the anterior region where expression is not detected. The mutant was missing the posterior-most expressing structure compared to wild type.
Limited protein expression was noted in collecting ducts located in the medullary region of the kidney, which had been co-labeled with the collecting duct marker dolichos bifloris agglutinin (DBA).
Expression was detected in the walls of the developing barrels. Diffuse staining was seen in the barrel hollows. The differential ratio of expression between the barrel walls and hollows appears to be less than at P7.
Expression was detected in the walls of the developing barrels. Diffuse staining was seen in the barrel hollows. The differential ratio of expression between the barrel walls and hollows appears to be less than at P7.
Expression in the dermomyotome of epithelialized somites was present in the caudal region whereas at the cervical and thoracic levels, expression was gradually restricted to the dorsal lip region.
A sharp decrease in fiber density compared with that of the E13 mutant, but to a level which was still significantly higher than that of the wild-type male (quantified in Fig 5B).
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 5D). There was also a decrease (about 50%) on the innervation density of the gland compared to PlexinA4 KO.
Expression was detected in interneurons in the P25 visual cortex. Some immunopositive cells were also labeled with Sst and/or Calb2. Single positive cells were observed in the deep layers.
Expression was detected in interneurons in the P25 visual cortex. Some immunopositive cells were also labeled with Nr2f2 and/or Calb2. Single positive cells were observed in the deep layers.
Cell counting analysis of expressing cells, summarized in Fig 7D. Only the ratio of positive cells in the layer VI was shown, since expression was only seen in the layer VI.
Expression was detected in a distinct cluster in the mantle layer immediately lateral to the ventral progenitor domain (VPD). The VPD is a region containing progenitor cells juxtaposed to the floor plate.
Expression was detected in a distinct cluster in the mantle layer immediately lateral to the ventral progenitor domain (VPD). The VPD is a region containing progenitor cells juxtaposed to the floor plate.
Expression was restricted to the caudal part of the ventral thalamus, the dorsal midline of the dorsal thalamus and pretectum, with a mediolateral expansion on the dorsal surface of the caudal pretectum.
Expression was restricted to the caudal part of the ventral thalamus, the dorsal midline of the dorsal thalamus and pretectum, with a mediolateral expansion on the dorsal surface of the caudal pretectum.
Expression was detected in the rostral half of the diencephalon in ventrolateral strips but was absent from the floor plate. The strips merged in the rostral-most diencephalon to the ventral floor plate.
Expression was detected in a stripe at the isthmus and dorsally it is expressed from the isthmus to part of the dorsal thalamus (p2 diencephalon). A negative region was noted in the anterior diencephalon.
Expression was more anteriorly located in the double heterozygote, but the stripe of expression was nearly unchanged. The negative region in the roof of the anterior diencephalon contained a line of expressing cells.
Expression was detected in the zona limitans intrathalamica as it extends toward the lateral surface of the diencephalic wall. Expression was in a narrow path situated on the dorsal side of a broad path containing the spindle-shaped cells (Arx-positive).
Expression was detected in the ventral thalamus, posterior entopeduncular area, hypothalamic cell cord, and the primodia of the suprachiasmatic area. The domain stops at the boundary with the postoptic area.
Expression was in the suprachiasmatic area, hypothalamic cell cord, posterior entopeduncular area and ventral thalamus. Caudal boundary was at the zone limitans. The negative region consisted of the eminentia thalami and several hypothalamic primordia.
Expression was in the suprachiasmatic area, hypothalamic cell cord, posterior entopeduncular area and ventral thalamus. Caudal boundary was at the zone limitans. The negative region consisted of the eminentia thalami and several hypothalamic primordia.
Expression was detected in the ventral thalamus and extended beyond the ventricular sulcus at the ventral/dorsal boundary. Positive cells extended processes from the ependymal limit to the pia mater border. The dorsal thalamus was negative.
Expression was detected in the ventral thalamus. Essentially all the cells were positive, at least at the ependymal layer level. The border of the positive cells at the ventral/dorsal thalamus and alar/basal boundary was sharp.
Expression was strong in the preoptic region, but there was no signal in the eminentia thalami. The pretectal alar plate, the basal plate portions of prosomeres 1-3, and the zona limitans intrathalamica had expression also.
Expression was detected in the basal plate of the hypothalamus, but no signal was seen in the dorsal thalamus. The pretectal alar plate and basal plate portions of prosomeres 1-3 had expression also.
Immunopositive fibers extend from the diencephalon toward the ventral surface of the pallidum. The bundle connects the dorsal thalamus and the basal-marginal telencephalon and was not detected in wild type.
Expressed within the neuroepithelium facing the optic pit. Abundantly expressed in a very limited zone where the two primary lateral evaginations, i.e., the optic stalks, arise, but was absent from the optic stalks.
Expressed in the perikaryon and cell processes of the neuroepithelium. Cells containing protein but not mRNA were also observed in the neuroepithelium. Protein but not mRNA was detected in neurons in the mantle zone.
Expressed around the ventricle. Expressed in a band of cells a short distance away from the apical surface of the ventricles. In addition, in cortex there was a second band of cells closer to the ventricle.
Expressed around the ventricle. Expressed in a band of cells a short distance away from the apical surface of the ventricles. In addition, in cortex there was a second band of cells closer to the ventricle.
Expression is at the lateral region of the mid-chiasm. Expression was reduced in the medial regions of the chiasm, except the midline, where an expression-rich raphe was observed extending from the chiasmatic neurons in the caudal regions of the diencephalon.
Expression is at the lateral region of the mid-chiasm. Expression was reduced in the medial regions of the chiasm, except the midline, where an expression-rich raphe was observed extending from the chiasmatic neurons in the caudal regions of the diencephalon.
Expressed in the prethalamus, but was absent from the zona limitans intrathalamica (ZLI). In the thalamus, expression levels were graded from low near the ZLI to high at the boundary with the pretectum.
Expressed in the prethalamus, but was absent from the zona limitans intrathalamica (ZLI). In the thalamus, expression levels were graded from low near the ZLI to high at the boundary with the pretectum.
Expression was maintained at the ventral midline, in a region directly dorsal to the site of axon divergence. No expression at the more ventral level at which the axons cross the midline.
Expression was maintained at the ventral midline, in a region directly dorsal to the site of axon divergence. No expression at the more ventral level at which the axons cross the midline.
Expression was maintained at the ventral midline, in a region directly dorsal to the site of axon divergence. Expressed by a subset of the midline radial glial cells (compared with 4V).
Expression was maintained at the ventral midline, in a region directly dorsal to the site of axon divergence. No expression at the more ventral level at which the axons cross the midline.
Expression was maintained at the ventral midline, in a region directly dorsal to the site of axon divergence. No expression at the more ventral level at which the axons cross the midline.
The onset of expression at E11.5 was observed in the most ventral aspects of tissue beneath the third ventricle. Expression was in a very small band of cells at the ventral edge of the Six3-expressing zone.
Neurons migrated extensively into r3. Although more numerous, appeared to remain largely within r3 (quantified in Fig 3J, S7D). There was a marked reduction in the size of the caudal migratory stream.
Expression was detected in endothelial cells of the thoracic duct. Dilated vessels in the mesenteric region, and vessel networks in the periorbital, lower jaw, and neck regions were positive. The absence of red blood cells indicated lymphatic endothelium.
Expression was detected in lymphatic endothelial cells. Data was presented in a graph of the number of immunopositive nuclei. The number of immunopositive cells was normal in the mutant embryos.
Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
Expression was detected in the Telenecephalic Vesicles, dorsal spinal cord and the laterovertebral mesenchyme. The latervertebral mesenchyme is defined as the cells located between the prevertebrae and the dorsal root ganglia.
The rostral boundary of expression in the mesoderm was found more caudal than that in the CNS. The anterior boundary in the somitic mesoderm was located around somite 11 and expression extended to the caudal end of the embryo.
The mesoderm exhibited a slightly weaker expression compared to the ectodermal cells in the primitive streak reagion. The signal was reduced further in mesodermal cells located anterior to the node, than in ectodermal cells of the developing neural tube.
Strong expression is present in the mesenchyme ventral to the rhombomeres and in the mesenchyme of the mesencephalic flexure. It was also located in the mesenchyme surrounding the primary brain vesicles, the spinal cord, and the embryonic heart.
The rostral boundary of expression in the mesoderm was found more caudal than that in the CNS. The anterior boundary in the somitic mesoderm was located around somite 11 and expression extended to the caudal end of the embryo.
Expression was in superficial fascia and epimysia of developing muscles of the anterior chest wall, in presumptive ligamentous attachments apposed to cartilage primordia of the exoccipital bone, basioccipital bone, vertebrae C1 and C2, and hip joint.
Expression was in superficial fascia and epimysia of developing muscles of the anterior chest wall, in presumptive ligamentous attachments apposed to cartilage primordia of the hip joint, and in the connective tissue sheath, or epineureum, of some nerves.
Expression was widely distributed throughout developing connective tissues with espicially high levels of expression seen in the perichondrium associated with cartilaginous primordia of future bones (vertebrae, ribs and bones of the hip joint).
Expression was in lateral mesenchymal cells adjacent to rhombomere 6 and which appear to form a stream of cells extending ventrally toward the developing third branchial arch. Only cells immediately adjacent to the surface ectoderm showed expression.
Expression was in lateral mesenchymal cells adjacent to rhombomere 6 and which appear to form a stream of cells extending ventrally toward the developing third branchial arch. Only cells immediately adjacent to the surface ectoderm showed expression.
Expression was detected in the cephalic and cervical regions. Expression in the cephalic flexure mesenchyme and surrounding the primary head and anterior cardinal vein and dorsal aorta was more intense.
Expression was detected in the cephalic and cervical regions. Expression in the cephalic flexure mesenchyme and surrounding the primary head and anterior cardinal vein and dorsal aorta was more intense.
Expression was detected in the blood islands of the yolk sac and in the heart and the aorta. Expression is increased in the extraembryonic yolk sac mesoderm and is high in aggregates of cells on the amnion.
Expression was detected in a crescent shape in the heart mesoderm with weakest expression in the differentiating myocardial and endocardial cells and strongest expression in the lateral arms of the crescent.
Expression was detected in a crescent shape in the heart field mesoderm with strongest expression in the lateral arms of the crescent - the prospective sinus venosus - and weaker in the more medial myocardial cells.
Expression was detected in a crescent shape in the heart field mesoderm with strongest expression in the lateral arms of the crescent - the prospective sinus venosus - and weaker in the more medial myocardial cells.
In the 6 mutants examined there was no difference between mutant and wild type; there is sparse noradrenergic immunoreactive innervation of the ventricular conduction system, the epicardium, and the coronary arteries.
Expression was detected in the majority of the cells of the cardiac crescent. A subset of these cells in the anterior and lateral regions were also positive for Tbx5. Expression was in the cytoplasm.
Expression was detected in the majority of the cells of the cardiac crescent. A subset of these cells in the posterior and dorsal regions were also positive for Isl1. Expression was in the cytoplasm.
Expression was detected in the sarcomere as a doublet. Treating the antibody with an acetone powder of mouse embryonic fibroblasts derived from knockout mice attenuated signal in the Z-line.
In the lower urogenital sinus, expression in the mesenchyme was detected immediately adjacent to the epithelium. In the upper urogenital sinus, expression was lower in the mesenchyme adjacent to the epithelium.
Expression was detected in the distal epithelial terminal buds. In the mutant, there appeared to be fewer cells with no change in their distribution (essentially unchanged from that seen at E15.5) than seen in wild type.
There is a reduction in the number of positive cells and decreased staining intensity in this mutant compared to wild-type. Expression was restricted to the nucleus in a few cells.
Positive cells were detected in the midbrain, and in condensations at the normal positions of the fifth and seventh cranial ganglia. The pattern of staining was similar to the 9.5-day wild-type embryo shown in Fig. 3A.
Transcripts showed a marked anterior-to-posterior fall in abundance over the anterior region of the spinal cord. This fall in abundance was anterior to prevertebra 1 in dorsal parts of the spinal cord but posterior to prevertebra 5 in ventral parts.
