The mutant protein is expressed at approximately equal levels and exhibits similar subcellular distribution to the wild type. As in wild type, expression was localized to the apical domain of adherens junctions in neural epithelial cells.
Expression was highest in the forebrain gradually decreasing in a rostrocaudal gradient to a region posterior to the forelimb bud, beyond which expression ceased. No dorsoventral gradient was visible.
Expressed in the ventricular and subventricular zones. In the intermediate and marginal zones expression was largely confined to the elongated cell bodies of radial glial cells. Strong expression in the region immediately outside the marginal zone.
By the late streak stage, expression was detected in an increased number of cells in the expanding blood islands and these cells formed a discrete band around the circumference of the visceral yolk sac.
Highly expressing cells were detected in a layer between primitive erythroid elements and the outer endoderm layer. Expression was also detected in the proximal region of the yolk sac.
Expression was detected in the mesenchyme, but not in epithelia. Expression was intense in the mesenchyme adjacent to the epidermis and in mesenchymal cell condensation located in front of growing hair follicles.
Expression was detected in motor neurons in the medial and lateral median motor columns and in the visceral motor column. The graph in fig. 4A showed a decrease over time.
Expression was detected in motor neurons in the medial and lateral median motor columns and in the visceral motor column. The graph in fig. 4A showed a decrease over time.
Expression was detected in motor neurons in the medial and lateral median motor columns and in the visceral motor column. The graph in fig. 4A showed a decrease over time.
Expression was in motor neurons. Expression in the visceral motor column was similar to wild type. No expression was in the medial and lateral median motor columns of the mutant. Graphed in Fig. 4A, 4C and 4D.
Expression was detected in motor neurons in the medial and lateral median motor columns and in the visceral motor column. The graph in fig. 4A showed a decrease over time.
Expression was detected in motor neurons in the medial and lateral median motor columns and in the visceral motor column. The graph in fig. 4A showed a decrease over time.
Expression was in motor neurons. Expression in the visceral motor column was similar to wild type. No expression was in the medial and lateral median motor columns of the mutant. Graphed in Fig. 4A, 4C and 4D.
Expression was detected in motor neurons in the medial and lateral median motor columns and in the visceral motor column. The graph in fig. 4A showed a decrease over time.
Expression was detected in motor neurons in the medial and lateral median motor columns and in the visceral motor column. The graph in fig. 4A showed a decrease over time.
Expression was detected in motor neurons in the medial and lateral median motor columns and in the visceral motor column. The graph in fig. 4A showed a decrease over time.
Expression was in motor neurons. Expression in the visceral motor column was similar to wild type. No expression was in the medial and lateral median motor columns of the mutant. Graphed in Fig. 4A, 4C and 4D.
Expression was detected in motor neurons in the medial and lateral median motor columns and in the visceral motor column. The graph in fig. 4A showed a decrease over time.
Expression was detected in motor neurons in the medial and lateral median motor columns and in the visceral motor column. The graph in fig. 4A showed a decrease over time.
Expression was in motor neurons. Expression in the visceral motor column was similar to wild type. No expression was in the medial and lateral median motor columns of the mutant. Graphed in Fig. 4A, 4C and 4D.
Dorsal-most expression was restricted to a few cells aligned along the outer edge of the ventricular zone in a discontinuous cell strand. Signal was divided into dorsal and ventral groups in the mantle layer.
Expression was detected in a stripe, several cell diameters wide on the side of the egg cylinder. The stripe extended proximally from the embryonic/extraembryonic junction almost to the distal tip and was on the opposite side of the primitive streak.
Expression was detected in the dorsal midline extending from the posterior diencephalon caudally through the entire body axis. The anterior expression limit was shifted caudally relative to the wild-type.
Weak expression was observed in 4 out of 5 embryos near the posterior third of the embryo. Staining was diffuse and not restricted to cells at the periphery of the node.
Fgf5 mRNA was not detected in embryos at this stage (E7.75), as determined by serial section analysis through the embryo, although it was abundant in embryos from the same litter that were not as advanced in development
Expression was detected in the floor plate and the ependymal layer of the spinal cord, but the cartilage primordium of the vertebral body and the degenerating notochord were negative.
Expression was detected in limb buds, branchial arches, nasal processes and brain. Staining was weak in the primitive spinal cord and the dorsal root ganglia. Other organs appeared negative.
Expression was present in the anterior half of the embryo, was absent from the cardiac progenitor region and no longer extended to the embryonic/extraembryonic junction: it was set back medially and fell away laterally with a relatively sharp boundary.
The X-gal expression pattern mimicked the in situ hybridization pattern (see assay MGI:1306682). The exception was staining in the notochord, which was clearly seen with X-gal staining, but was barely detectable by in situ.
Expression was highly expressed in 2 regions. Staining in the lateral region extended laterally along the anteroposterior axis. The second region was near the ventral midline. Expression was only on the left side.
Expression was detected in a sheet of epitopes separating the medial aspect of the primitive lungs from the mediastinal mesenchyme. Expression was detected within individual mesenchymal cells and in the adjacent extracellular matrix.
Staining is similar to that observed at E10.5. At that time it was seen in the ear, heart, somites, branchial arch, central nervous system, and presomitic mesoderm and/or trunk.
Staining is similar to that observed at E10.5. At that time it was seen in the ear, heart, somites, branchial arch, central nervous system, and presomitic mesoderm and/or trunk.
In female embryos at E7.5 cells no longer showed low level biallelic Xist expression. A proportion of cells displayed differential biallelic Xist expression, and in the majority of cells, abundant Xist RNA was detected from only one locus.
In female embryos at E8.0 a proportion of cells displayed differential biallelic Xist expression, but in the majority of cells abundant Xist RNA was detected from only one locus.
Staining spread to more anterior levels encompassing a larger area than that at the previous stage. Anterior boundary staining was still weak and restricted to a region posterior to the limit of somite condensation.
Expression was visible in almost every tissue of the embryos and appeared to be primarily staining the extracellular matrix and the cell surface. Staining was lowest in this sample.
Expression was visible in almost every tissue of the embryos and appeared to be primarily staining the extracellular matrix and the cell surface. Staining was highest in this sample.
Expressed in the anterior region. The axis passing through the center of the two expression domains (Fgf8/Cer1) was oriented toward being parallel to the long axis of the embryo.
Expressed in the anterior region. The axis passing through the center of the two expression domains (Fgf8/Cer1) was oriented toward being parallel to the long axis of the embryo.
Expressed in the anterior region. The axis passing through the center of the two expression domains (Fgf8/Cer1) was oriented toward being parallel to the long axis of the embryo.
Expressed in the posterior region. The axis passing through the center of the two expression domains (Fgf8/Cer1) was oriented toward being parallel to the long axis of the embryo.
Expressed in the posterior region. The axis passing through the center of the two expression domains (Fgf8/Cer1) was oriented toward being parallel to the long axis of the embryo.
Expressed in the posterior region. The axis passing through the center of the two expression domains (Fgf8/Cer1) was oriented toward being parallel to the long axis of the embryo.
Expression was detected in the epithelial cells of the crypt and along the villi at regions of cell-cell contact but was absent from the apical surface of the epithelial cells.
Expression was detected in all epithelial cells except for Paneth cells and goblet cells. There were graduations in intensity with weaker staining in the upper parts of the villus and in the crypts.
Expression was detected in a mosaic pattern in the heterozygous female. Absorptive cells in the villus portion were aggregates of either positive or negative expression. Crypts were occupied exclusively of positive- or negative-staining cells.
Expression was detected in a mosaic pattern in the heterozygous female. Absorptive cells in the villus portion were aggregates of either positive or negative expression. Crypts were occupied exclusively of positive- or negative-staining cells.
Expression was detected in patches in the presumptive crypts, and the patches were larger than those at E17.5. The crypts were composed of both positive and negative staining cells.
Expression was detected in patches in the presumptive crypts, and the patches were larger than those at E17.5. The crypts were composed of both positive and negative staining cells.
Expression was detected in the crypts. Crypts mostly contained cells exclusively stained either positive or negative. Some crypts were noted that contained mixed staining. Some small positive patches were also seen near the base part of the villus.
Expression was detected in the crypts. Crypts mostly contained cells exclusively stained either positive or negative. Some crypts were noted that contained mixed staining. Some small positive patches were also seen near the base part of the villus.
Expression was confined to the columnar epithelium covering the plicae and villi projecting into the intestinal lumen. No expression was detected in the sub-epithelial mesenchymal part of these projections.
Most tips had developed an irregular shape and showed numerous indentations (star-shaped pattern), likely representing sites of fusion with adjacent early nephrons. Expression was excluded from the connection point with the "invading" renal vesicles.
Strongly expressed in the cortex, in uninduced mesenchymal cells in the nephrogenic zone and in ureteric bud tips. A weaker signal was detected in mesenchymal cells in the medulla and around the ureter.
Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.
Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.
Thick bands of expression radiated from the foot and along the digit borders. Expression was also visible at presumptive joint sites along the digits, but was not observed in the distal digit tips.
Thick bands of expression radiated from the foot and along the digit borders. Expression was also visible at presumptive joint sites along the digits, but was not observed in the distal digit tips.
Expression was detected throughout the rostrocaudal extent of R3 and R5 neuroepithelium, most prominent dorsally and at the rhombomere boundaries. Neuroepithelium expression was also detected in a continuum from R1 into the forebrain.
Expression was detected in the mutant embryos as in wild type, but the head vessels were dilated in the mutant and had less complex branching in comparison with the wild type.
The vascular morphology varies in mutants compared to wild type. In those with the bulbous allantois, the vasculature was less severely affected, and the structures like dorsal aortae and intersomitic vessels are visible, albeit hypomorphic.
Expression was detected in blood vessels in the perichondrium of the tibia and the surrounding tissue, as in wild type. The entire cartilaginous shaft is devoid of blood vessels.
Expression was detected in a dilated vessel in the axillar region, in a plexus of vessels in the periorbital region, surrounding the developing vertebrae, and in a vascular network beneath the skin. Weak capillary signals were noted in several regions.
Expression is in a discrete punctuate pattern and was localized in the cranial region, between the mesonephric duct and the gonad (for both male and female, unsexed specimen is shown).
Expression was detected at the anterior end of the mesonephros of the mutant, but the positive cells remain as a small cluster rather than invaginate for tubulogenesis of the Mullerian duct.
Expression was detected at the anterior end of the mesonephros of the mutant, but the positive cells remain as a small cluster rather than invaginate for tubulogenesis of the Mullerian duct.
Expression was detected in projection neurons (strong in layer V and weak in layer VI) in the motor cortex. No significant changes in immunopositive cell number was noted compared with wild type.
Expression was detected in projection neurons (strong in layer V and weak in layer VI) in the motor cortex. No significant changes in immunopositive cell number was noted compared with wild type.
Expression was detected in projection neurons (strong in layer V and weak in layer VI) in the motor cortex. No significant difference in immunopositive cell number was noted compared with wild type.
Expression was detected in projection neurons (strong in layer V and weak in layer VI) in the motor cortex. No significant difference in immunopositive cell number was noted compared with wild type.
Expression was strong in the osteoblasts in bone marrow, the bone collar covering the layers of proliferating and hypertrophic chondrocytes, bone marrow and the terminal hypertrophic chondrocytes but was weak in the resting and proliferating chondrocytes.
rostral expression; Small region of strong expression on the rostral tip of the male mesonephros. Expression maybe associated with the paramesonephric duct as expression was seen in the female paramesonephric duct.
Transcripts within the mesoderm were localized to the posterior half of the embryo, just lateral to the primitive streak. The anterior boundary of transcripts was rostral to the archenteron.
A girdle of labelling was present in the proximal mesodermal wings, excluding the primitive streak, in the form of a horse-shoe surrounding the remaining proximal area of the epiblast. Signal was localized in the anterior-proximal embryonic mesoderm.
Fgf5 was not detected in cells that had migrated either proximally into the extraembryonic region or proximolaterally between embryonic ectoderm and visceral endoderm. Cells exiting the streak more distally expressed Fgf5 similarly to distal ectoderm.
No expression was detected in proximolateral mesoderm, whereas distal mesoderm was positive (roughly the same levels as in adjacent ectoderm). Head process cells 9cells that have migrated anterior to the distal end of the streak) were also positive.
A girdle of labelling was present in the proximal mesodermal wings, excluding the primitive streak, in the form of a horse-shoe surrounding the remaining proximal area of the epiblast. Signal was localized in the anterior-proximal embryonic mesoderm.
A more anterior expression boundary was noted in the neural tube than in the mesoderm. Expression in the mesoderm was located more posterior in the still unsegmented region of the embryo.
Expression detected in presumptive S2 and S3 segments. Note that only weak expression was detected in the S1 segment adjacent to renal corpuscle and only in a subset of nephrons.
Expression was more prominent in the posterior and dorsal part of the forelimb bud. In the anterior part, expression was restricted to dorsally and distally located cells, while more posteriorly, the boundary was located progressively more ventrally.
Expression was more prominent in the posterior and dorsal part of the forelimb bud. In the anterior part, expression was restricted to dorsally and distally located cells, while more posteriorly, the boundary was located progressively more ventrally.
Expression was more prominent in the posterior and dorsal part of the forelimb bud. In the anterior part, expression was restricted to dorsally and distally located cells, while more posteriorly, the boundary was located progressively more ventrally.
Authors report that expression was only modestly altered from the wild type pattern, being perhaps slightly elevated in the posterior and distal edge of the limb relative to wild type.
Expression was detected in an asymmetrical pattern enriched towards the anterior mesenchyme compared to the posterior. Comparison to Mki67 staining indicates that Lhx9 is expressed in many proliferative cells.
Expression was detected in the progress zone mesenchyme and the proximal dorsomedial mesenchyme of the forelimb. Expression in the progress zone area was more restricted to the dorsal side.
Expression was detected in the progress zone mesenchyme and the proximal dorsomedial mesenchyme of the forelimb. Expression in the progress zone area was more restricted to the dorsal side.
Expression was detected in the progress zone mesenchyme and the proximal dorsomedial mesenchyme of the forelimb. Expression in the progress zone area was more restricted to the dorsal side.
Expressed in the distal mesenchyme and in regions of condensing cartilage, being more abundant in the posterior domains. An anterior expression domain was also observed at the base of the limb buds.
Expression was restricted to the superficial limb mesenchyme, both dorsally and ventrally, and excluded from the central chondrogenic (precartilaginous) condensations. Expression was not detected in the distal tip. Expression was outside of the progress zone.
Expression was detected in the interposed and lateral deep cerebellar nuclei. High magnification of double staining showed cells that were immunopositive for both Olig2 and Pou3f2, as well as other cells that were only Olig2-positive.
The core mesoderm of the allantois contained Flk-1-positive cells. flk-1 was not observed in the mesothelium of the allantois. Flk-1 protein was abundant in flattened cells that formed obvious vascular channels throughout the allantois.
Expression was detected in the blood islands of the yolk sac and in the heart and the aorta. Expression is increased in the extraembryonic yolk sac mesoderm and is high in aggregates of cells on the amnion.
Expression was detectable in trophoblast cells at the periphery of the ectoplacental cone. Transcripts were absent or present at much lower levels in the proliferative trophoblast of the chorion. Expression was not detectable in trophoblast giant cells.
In spongiotrophoblast, signal was discontinuous, restricted to fraction of cells. Expression in focal areas of labyrinthine layer, not detectable in trophoblast giant cells. Downregulation at border between spongiotrophoblast and trophoblast giant cells.
Authors state that there was no expression in the visceral endoderm component of the yolk sac, except at the marginal zone near the ectoplacental cone, where visceral endoderm remains in contact with parietal endoderm.
Staining was detected at the interface of the embryonic ectoderm and overlying visceral endoderm. The syndecan present in this location is likely produced by the extraembryonic ectoderm and localized at the interface of the two cell layers.
Staining was detected at the interface of the embryonic ectoderm and overlying visceral endoderm. The syndecan present in this location is likely produced by the extraembryonic ectoderm and localized at the interface of the two cell layers.
Staining was detected at the interface of the embryonic ectoderm and overlying visceral endoderm. The syndecan present in this location is likely produced by the extraembryonic ectoderm and localized at the interface of the two cell layers.
There was no overlap of the Xist expression domain with the Atrx protein in the two nuclei from chorion shown in the figure. Only 5.8% of chorion nuclei show partial overlap of expression.
