At E16.5, there was no difference in the distribution of immunopositive cells between control and mutant mice, and the total number of labeled neurons was similar in the two genotypes.
At E16.5, there was no difference in the distribution of immunopositive cells between control and mutant mice, and the total number of labeled neurons was similar in the two genotypes.
Cell counting analysis of expressing cells, summarized in Fig 7D. Only the ratio of positive cells in the layer VI was shown, since expression was only seen in the layer VI.
Cell counting analysis of expressing cells, summarized in Fig 7D. Highest expression. Only the ratio of positive cells in the layer VI was shown, since expression was only seen in the layer VI.
Cell counting analysis of expressing cells, summarized in Fig 7D. Decreased expression compared with that of P14. Only the ratio of positive cells in the layer VI was shown, since expression was only seen in the layer VI.
Expression was restricted to tips of the epithelial tubules, areas displaying high levels of Collagen IV mRNA. Expression on more mature central airway epithelium was greatly diminished to undetectable.
Expression was localized at the apical side of distal epithelial cells during apical-basal cell division and have a diffuse localization in cells that are in interphase or are undergoing lateral divisions.
Expression was localized at the apical side of distal epithelial cells during apical-basal cell division and have a diffuse localization in cells that are in interphase or are undergoing lateral divisions.
Expression was localized at the apical side of distal epithelial cells during apical-basal cell division and have a diffuse localization in cells that are in interphase or are undergoing lateral divisions.
Expression was detected in approximately half of the proximal airway epithelial cells at E15.5. The ciliated cell marker Tubb4 was first detected at E15.5 and was restricted to a small number of airway epithelial cells that express Foxj1.
Expression was strong in the presomitic mesoderm, but was undetectable in the mesenchyme underlying the anterior neural ectoderm. The change in expression was fairly abrupt, and occurred at the level of the otic sulcus.
Expression was strong in the presomitic mesoderm, but was undetectable in the mesenchyme underlying the anterior neural ectoderm. The change in expression was fairly abrupt, and occurred at the level of the otic sulcus.
Expression was detected in the organ of Corti at the basal end of the cochlea in a single row of cells adjacent to the lumen of the cochlear duct, corresponding to the inner hair cells.
Expression was detected in the organ of Corti at the basal end of the cochlea in a single row of cells adjacent to the lumen of the cochlear duct, corresponding to the inner hair cells.
Expression was expanded medially into the greater epithelial ridge and maintained in support cells (inner phalangeal cells, pillar cells, Deiters' cells, Hensen's cells). Expression is lost from the nuclei of hair cells, but persists in cytoplasm.
Expression was detected in the organ of Corti at the basal end of the cochlea in a single row of cells adjacent to the lumen of the cochlear duct, corresponding to the inner hair cells.
Strong expression was detected in the apical portion of the outer hair cells and greater epithelial ridge, but not inner hair cells. Outer hair cell distribution corresponded to the location of adherens junctions. Expression was in pillar cells.
The ring-shaped stereocilin labeling at the tips of the tallest stereocilia was not detected in this mutant, whereas labeling of stereocilia from the middle and short rows was present.
Expression was detected in radially oriented stripes that fanned out to form a diffuse spiral band in the spiral lamina. Puncta of immunoreactivity were detected in the organ of Corti along the inner pillar cells.
Expression was detected in the inner and outer hair cell regions in the basal turn of the organ of Corti. Small and large puncta of label were beneath outer hair cells, and Deiters' cells were outlined with label.
There was a limited amount of labeling in the inner hair cell region of the apical turn of the organ of Corti. No label was in the outer hair cell region.
There was a base-to-apex gradient of expression in hair cells that extended to the middle turn. Expression level was low in inner hair cells and stronger in outer hair cells in the basal turn.
Expression was detected at a moderate level in inner hair cells and at a stronger level in outer hair cells. There was strong expression in the basal turns and low level expression in the middle turn.
Authors report expression in cochlear floor was severely reduced in homozygote compared to heterozygote control. Expression retained in abneural cells in the sensory domain, and non-epithelial cells outside the cochlear duct.
Staining was adjacent to the myenteric plexus (of Meissner) and the lack of staining internal to muscularis mucosa, suggests that it is not associated with the mucosal plexus of Auerbach.
Expressed in the outer enamel epithelium and inner enamel epithelium. Expression in the region near the cervical loop was weaker than that in the other regions at the presumptive cusp sites.
Expressed in the outer enamel epithelium and inner enamel epithelium. Expression in the region near the cervical loop was weaker than that in the other regions at the presumptive cusp sites.
Highly expressed in all lobules in the vermis and hemisphere. Expression was less intense in the nodulus (vermal lobule X). Only mildly expressed or not expressed in the relatively lateral small area in the nodulus.
Highly expressed in all lobules in the vermis and hemisphere. Expression was less intense in the nodulus (vermal lobule X), and the bottom of the fissure between lobules III and IV.
Expression is in one compartment located caudally, a negative compartment was located in the central part, and a compartment with sparse distribution of mildly positive Purkinje cells was located in the rostral part.
Expression is in one compartment located caudally, a negative compartment was located in the central part, and a compartment with sparse distribution of mildly positive Purkinje cells was located in the rostral part.
Rostral aorta. At this stage, expression was found dispersed throughout the wall of the major blood vessels. Expression was within the developing smooth muscle cell wall of major blood vessels.
Morphological abnormality of the epithelial sheet was first observed, although the 1st bending of the endoderm sheet was observed. The epithelial sheet did not maintain the monolayer structure at the bending site.
Expression in the cytocortex was detected in the apical microvillous pole on the outer surface of each blastomere in 85% of the embryos but absent from the nascent apicolateral tight junctions site.
Expression in the cytocortex was in the apical microvillous pole in outer blastomeres of the trophectoderm lineage of 67% of embryos. Faint zonular or discontinuous staining was in the forming tight junction sites of each blastomere in 75% of embryos.
Expression was absent from the inner cell mass and the cytocortex, but was present in the tight junctions in the trophectoderm in 100% of embryos and in punctate cytoplasmic foci surrounding the trophectoderm nuclei in 70% of embryos.
Expression was brightest in the apical region of outer blastomeres, but was not seen on plasma membrane in the inner blastomeres. Staining had a granular appearance throughout the cytoplasm.
Nuclear expression diminished in intensity with gradually more nuclei becoming unlabeled in late cleavage. Distinct membrane staining was first detected at the 16-cell stage as a discontinuous linear array of spots at cell boundaries between outer (trophectoderm lineage) blastomeres.
Expression was restricted to a central area in the branchial arch, and in the dorsal part showed a perforated appearance in the stapedial condensation, which lies in close proximity to the otic vesicle.
The altered expression was particularly evident in the dorsal part, where it was no longer close to the otic vesicle but more ventrally located, adjacent to the first pharyngeal pouch.
There was no expression in the epithelial layer of the branchial arch or in migratory neural crest cells. Transcripts were concentrated in the neural-crest-derived mesenchyme just underlying the most exterior portion of the branchial arches.
Intense labeling anteriorly throughout the mesenchyme that leveled off in the middle third and the posterior third was barely above background. More intense labeling in the top (oral) half.
Intense labeling anteriorly throughout the mesenchyme that leveled off in the middle third and the posterior third was barely above background. More intense labeling in the top (oral) half.
Expression was detected in most of the mesenchymal cells in the lateral region of the first branchial arch mandibular component. A core of mesenchymal cells do not show expression.
Expression was detected in most of the mesenchymal cells in the lateral region of the first branchial arch mandibular component. A core of mesenchymal cells do not show expression.
Expression was detected in most of the mesenchymal cells in the lateral region of the first branchial arch mandibular component. A core of mesenchymal cells do not show expression.
Expression was detected in most of the mesenchymal cells in the lateral region of the first branchial arch mandibular component. A core of mesenchymal cells do not show expression.
Expression in the developing nephron was continuous with that of the ureteric bud, beginning at the junction with the bud and extending proximally to, but excluding, the most proximal segment of the S-shaped body.
A distinct domain of expression in the paraxial mesoderm was observed immediately prior to the last-formed somite, which is separated from the rest of the paraxial mesoderm by a non-expressing domain.
A distinct domain of expression in the paraxial mesoderm was observed immediately prior to the last-formed somite, which is separated from the rest of the paraxial mesoderm by a non-expressing domain.
No difference in expression pattern was seen in the cervical region. Expression was seen up to the thoracic region and could not be observed in the lumbar, sacral or caudal regions.
Authors state that Pax3 expression patterns (in the future dermomyotome) revealed that paraxial mesenchyme in the thoracic region becomes properly segmented. However, in dorsal somitic regions, an abnormal, flame-like pattern of expression was observed.
Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm. All cells that were immunopositive were also positive for the expression of MyoD (see Assay MGI:3611794).
Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm. Only a subset of cells that were immunopositive were also positive for the expression of Myogenin (see Assay MGI:3611793).
A distinct domain of expression in the paraxial mesoderm was observed immediately prior to the last-formed somite, which is separated from the rest of the paraxial mesoderm by a non-expressing domain.
A distinct domain of expression in the paraxial mesoderm was observed immediately prior to the last-formed somite, which is separated from the rest of the paraxial mesoderm by a non-expressing domain.`
Expression was present in four stripes in the paraxial mesoderm. The weaker anteriormost stripes mark the rostral region of the most recently formed somites, while the stronger posterior stripes are within the presomitic mesoderm.
Expression was detected in the paraxial mesoderm at the correct distance from the tail bud. The signal occupied a rostrocaudally broadened, but dorsoventrally shortened domain with poorly defined borders.
Expressed in the presternum. Rib 1 appeared to make contact with the rounded, medial region. Sternal bar fusion was also incomplete, with a loose collection of Sox9-expressing cells extending between them.
Co-expressed with Sox9 in the presternum. Rib 1 appeared to make contact with the rounded, medial region. Sternal bar fusion was also incomplete. None of these presternum elements showed contribution from Meox1-Cre labelled somite derived cells.
Co-expressed with Sox9 in the presternum. Rib 1 appeared to make contact with the rounded, medial region. Sternal bar fusion was also incomplete. None of these presternum elements showed contribution from Meox1-Cre labelled somite derived cells.
Co-expressed with Sox9 in the presternum. Rib 1 appeared to make contact with the rounded, medial region. Sternal bar fusion was also incomplete. None of these presternum elements showed contribution from Meox1-Cre labelled somite derived cells.
Expressed in the presternum. Rib 1 appeared to make contact with the rounded, medial region. Sternal bar fusion was also incomplete, with a loose collection of Sox9-expressing cells extending between them.
Expressed in the presternum. Rib 1 appeared to make contact with the rounded, medial region. Sternal bar fusion was also incomplete, with a loose collection of Sox9-expressing cells extending between them.
Presternum expression was seen in separate dorsal and ventral presternal elements, which were Meox1-Cre lineage/RFP-negative. Rib 1 contacted the paired ventral elements that have not yet fused or fully condensed into the bar shape observed later.
Presternum expression was seen in separate dorsal and ventral presternal elements, which were Meox1-Cre lineage/RFP-negative. More posteriorly, the site of rib 1 attachment transitioned to the plow-shaped, dorso-ventrally elongated morphology characteristic of the sternal bars.
Presternum expression was seen in separate dorsal and ventral presternal elements, which were Meox1-Cre lineage/RFP-negative. Rib 2 similarly contacted the unfused sternal bars, and the bars were connected by a stream of Sox9-expressing cells.
In this gonad expressing cells are present at the anterior pole (pictured), as well as the posterior pole. This was the only gonad at this stage observed to have this staining pattern.
This mutant has a ovotestes composed of testicular cells and ovarian cells. The ratio of FOXL2-positive cells to SOX9-positive cells was increased in Tet2/Kdm3a-double deficient gonads as compared to Kdm3a-deficient gonads.
This mutant has a ovotestes composed of testicular cells and ovarian cells. The ratio of FOXL2-positive cells to SOX9-positive cells was increased in Tet2/Kdm3a-double deficient gonads as compared to Kdm3a-deficient gonads.
Expression was normal at the transition between choroid epithelium and cerebellar neuroepithelium. However expression was dramatically extended in the transition zone between the choroid epithelium and neuroepithelium of the spinal cord.
Expression was detected in the cilia, which were identified by anti-acetylated tubulin. No GFP fluorescence was observed in nonciliated cells, and no GFP fluorescence was seen in the cytoplasm of ciliated cells.
Expression was detected in very small patches in the small intestine of the heterozygous mutant and with no definite orientation in the villus structures as seen in the adult.
Expression was detected in patches in the villus of the small intestine. Fig. 5 was a set of serial sections showing that positive patches in a villus were often connected with those of the adjacent villus, but not always.
Expression was detected in patches in the villus of the small intestine. Fig. 5 was a set of serial sections showing that positive patches in a villus were often connected with those of the adjacent villus, but not always.
Expression was detected in patches in the villus of the small intestine. Fig. 5 was a set of serial sections showing that positive patches in a villus were often connected with those of the adjacent villus, but not always.
Expression was detected in patches in the villus of the small intestine. Fig. 5 was a set of serial sections showing that positive patches in a villus were often connected with those of the adjacent villus, but not always.
Expression was detected in patches in the villus of the small intestine. Fig. 5 was a set of serial sections showing that positive patches in a villus were often connected with those of the adjacent villus, but not always.
Ventricular zone expression was detected along a transverse line aligned precisely with the IMB (isthmo-mesencephalic transverse boundary), that is, in between the evaginated Fgf8-positive isthmus and the sulcal midbrain area (both of which lacked Wnt1 signal).
Ventricular zone expression was detected along a transverse line aligned precisely with the IMB (isthmo-mesencephalic transverse boundary), that is, in between the evaginated Fgf8-positive isthmus and the sulcal midbrain area (both of which lacked Wnt1 signal).
Expression was detected in migrating mesendodermal wings arising in the anterior region of the streak. The domain next to the primitive streak expanded and extended more into the anterior half of the embryo.
Expression was detected in clusters of cells. Compared to E16.5, the number of clusters increased with a concomitant decrease in the number of cells per cluster (seen at higher magnification in inset).
Cell counting analysis of expressing cells, summarized in Fig 7D. Only the ratio of positive cells in the layer VI was shown, since expression was only seen in the layer VI.
Cell counting analysis of expressing cells, summarized in Fig 7D. Highest expression. Only the ratio of positive cells in the layer VI was shown, since expression was only seen in the layer VI.
Cell counting analysis of expressing cells, summarized in Fig 7D. Decreased expression compared with that of P14. Only the ratio of positive cells in the layer VI was shown, since expression was only seen in the layer VI.
In seven Sd homozygotes the number of expressing sclerotomes starting from the proatlas anlage was 17 on average. This is similar to what was observed in day 13.5 wild-type embryos.
A cluster of cells was detected just dorsal to the maxillary branch of the first branchial arch and just caudal to the eye. The dorsal-lateral surface of the head is lined with positive cells.
The overall expression level of mutant embryos was reduced compared to wild type. This reduction in expression was most readily apparent in the posteriormost regions of the mutant in the neural folds and lateral mesoderm.
Expression was detected throughout the entire width of the neural plate and tube. Expression was more intense where tube was not yet closed. Signal was stronger near the lumen of the tube.
Expression is absent in the most dorsal and ventral cells, as well as the cells adjacent to the floor plate. Expression was not seen in the more caudal regions of the future spinal cord.
Expression was detected throughout the entire width of the neural plate and tube. Expression was more intense where tube was not yet closed. Signal was stronger near the lumen of the tube.
Expression was largely in the cytoplasm of cells in the trunk neural tube. A very small portion of staining was present in the nucleus and some of it colocalizes with the nucleolar marker Npm1 (B23).
Expression was detected in the neuroepithelium of the ventral regions largely overlapping with the Neurog2 expression domain in the dorsoventral axis. Expression extends ventrally up to the floor plate and was not detected in the emerging marginal zone.
Expression was high in a broad band of ventricular zone precursors in the dorsal half of the neural tube. A much smaller band of expression was detected in the ventral-most region. Expression overlapped with Mash1 in the dorsal spinal cord.
Expression was strong in the dorsal half of the neural tube. The abnormal dorsal expression was throughout the length of the neural tube except in the anterior trunk regions, where the roof plate was devoid of expression.
The expression level was highest at the pial side. The majority of staining was observed outside motor neurons and the fiber tracts in the mantle layer, but was clearly inside the mesenchymal cells surrounding the neural tube.
A rostral expansion was seen compared with that of E10.5. The degree of the shift was much less than observed for the other 5' Hoxb genes. The arrowhead marked the anterior boundary.
A dramatic rostral expansion was seen compared with that of E10.5. The anterior boundaries of expression was similar to Hoxb paralogs at both stages. The arrowhead marked the anterior boundary.
A dramatic rostral expansion was seen compared with that of E10.5. The anterior boundaries of expression was similar to Hoxb paralogs at both stages. The arrowhead marked the anterior boundary.
A more anterior expression boundary was found in the neural tube than in the mesoderm. The anterior boundary of expression in the neural tube was found at the level of the 9th or 10th somite.
