Expression was detected in a more anterior location up to the level of the 4th prevertebra. No expression was found in the ventral horns or ependymal layer at this axial level.
Expression was highest in the nuclei of cells in the ventral horn regions of the rostral neural tube. Weaker immunoreactivity was observed in apical cells of the dorsal neural tube.
Expression was highest in the nuclei of cells in the ventral horn regions of the rostral neural tube. Weaker immunoreactivity was observed in apical cells of the dorsal neural tube.
flk-1 was not observed in the mesothelium of the allantois. The percentage of positive core cells was highest in the distal third of the allantoic bud, significantly lower in the basal third and intermediate in the middle third.
flk-1 was not observed in the mesothelium of the allantois. The percentage of positive core cells was highest in the distal third of the allantoic bud, significantly lower in the basal third and intermediate in the middle third.
flk-1 was not observed in the mesothelium of the allantois. Expression had become abundant in the basal third of the allantois and was invariably expressed in the nascent allantoic blood vessel found at the allantoic/yolk sac junction at 4-somite pairs.
Expression was detected in the proximal region of the allantoic core domain. An occasional positive cell was noted in the distal allantoic core domain, but never in the extreme distal allantois at this stage.
Precommissural level of expression was similar to that in wild-type; however, expression did not increase at the ventral midline. Weak expression was seen in some regions of the ventral funiculus.
As in wild-type, expression is seen on commissural axons projecting ventrally towards the midline. In contrast to wild-type, expression was also seen on the (rare) longitudinally directed commissural axon.
As in wild-type, expression is seen on commissural axons projecting ventrally towards the midline. In contrast to wild-type, expression was also seen on the (rare) longitudinally directed commissural axon.
As in wild-type, expression is seen on commissural axons projecting ventrally towards the midline. In contrast to wild-type, expression was also seen on the (rare) longitudinally directed commissural axon.
Expression was detected in postmitotic neurons in the cervical spinal cord. The signal did not exceed background levels in the germinal epithelium, while expression was strong in the developing ventral horn containing the differentiating motor neurons.
Expression was detected in a dorsal population of ventricular cells that includes the roof plate and in 1-2 cells located near the boundary of the alar and basal plates.
The number of neurons was significantly reduced in the mutant. The C7-C8 region of the spinal cord was less strongly affected, but the C5 and C6 regions showed a massive reduction in the number of immunopositive motor neurons.
In the double mutant, the neurectodermal anterior expression boundary was shifted posteriorly by the extent of one somite level. The boundary was located at a level halfway up the sixth somite.
Expression was detected in the dorsal third of the spinal cord including the dorsal root entry zone and the roof plate. Expression was also noted in the motor neurons.
Expression was detected in the dorsal third of the spinal cord including the dorsal root entry zone and the roof plate. Expression was also noted in the motor neurons.
Expression was detected in the ventricular zone. There was a gap in the ventral expression that corresponds to the En1 expression in the intermediate region. Dorsal to the gap, expression is continuous up to the roof plate.
In the posterior spinal cord, expression was detected in postmitotic neurons migrating out of the ventricular zone. Cells were located at the lateral margins of the ventricular zone and in a few cells close to the lumenal surface.
Expression was detected in differentiating neurons at the lateral margins of the ventricular zone in two domains; dorsal to the ventral horn in the ventral spinal cord and extending up to the sulcus limitans, and in the developing dorsal horn.
Expression was detected in interneurons migrating out of the ventricular zone and ventrally. Expression was detected in cells near the marginal zone lateral to motoneurons in the lateral motor column.
Expression was detected in interneurons migrating out of the ventricular zone and ventrally. Expression was detected in cells near the marginal zone lateral to motoneurons in the lateral motor column. Expression was indistinguishable from wild type.
Dense staining was observed in the dorsal-most spinal cord. A dorsal-to-ventral stand of cells forms from the dorsal domain to the ventral-most level. In the strand, dorsal cells are subpial, while ventral cells course medially to the ventral horn.
A new LacZ expression pattern was detected in the spinal cord. Expression was observed in fibers originating in the alar plate, elongating ventrally, and crossing the ventral midline (commissural axons).
A new LacZ expression pattern was detected in the spinal cord. Expression was observed in fibers originating in the alar plate, elongating ventrally, and crossing the ventral midline (commissural axons).
Expression was detected in the ventral midline, where it colocalized with Shh in situ hybridization. Low levels of expression were noted in presumptive motor neurons, which do no express Shh.
Expression was detected as a longitudinal stripe along the dorsal area of the alar plate, but not in the roof plate. Expression extended for the entire length of the spinal cord.
The total number of BrdU+Mki67+ cells in the mutants was reduced by 27% compared with wild-type siblings when a 16h BrdU pulse was administered. In addition, cell cycle exit indices (BrdU+Ki67-: BrdU+Ki67+) were increased by ~60% compared with wild-type littermates.
Venous-specific staining for the limb skin vasculature. Overlapped with EphB4-lacZ expression in large-diameter veins, whereas expression was much more clearly detectable in branched middle-diameter veins and venous capillaries than EphB4-lacZ expression.
The expression was broad in the nasal side and narrow in the oral side, but was hardly seen in the mesenchyme on either side of the medial edge epithelium seam.
Signals were at lower levels in the lesser epithelial ridge. In the greater epithelial ridge, the expression pattern partly overlapped with that of Thrb (see assay MGI:5311181) and decreased as the tissue was lost between P3 and P15.
Signals were at lower levels in the lesser epithelial ridge. In the greater epithelial ridge, the expression pattern partly overlapped with that of Thrb (see assay MGI:5311181) and decreased as the tissue was lost between P3 and P15.
The strongest labeling in the inner hair cell region was between the inner sensory cells and the inner pillar cells. Large accumulations of label were detected beneath the synaptic region of outer hair cells.
The strongest labeling in the inner hair cell region was between the inner sensory cells and the inner pillar cells. After birth fewer and fewer labeled puncta can be detected around the Deiters' cells.
At a slightly higher level than in 2G, the apical ends of the pillar cells were labeled. Outer hair cells were unlabeled. Labeling was on the modiolar side of inner hair cells in the basal turn.
Expression was detected in hair cells only in the most apical turn of the organ of Corti. Expression was absent from all hair cells in the middle and basal turns.
Expression was detected in hair cells only in the most apical turn of the organ of Corti. Expression was absent from all hair cells in the middle and basal turns.
Expression was detected in the proximal regions of cilia in both hair-cell types and supporting cells. Magnified inset shows ciliary immunolocalization in a single outer hair cell (arrow) and an adjacent Deiters' cell (arrowhead).
Expression was present in the arcuate nucleus. Labeling was most comparable to sections from wild type XX mice by qualitative visual inspection of the optical density of reaction product.
Expression was present in the arcuate nucleus. The labeled cells in XY wild type animals were consistently lighter in color than in either XX wild type or XY Sf1 KO mice.
There was no expression in the epithelial layer of the branchial arch or in migratory neural crest cells. Transcripts were concentrated in the neural-crest-derived mesenchyme just underlying the most exterior portion of the branchial arches.
Expression was detected in neural crest cells in the first arch from rhombomere 4. The labeled neural crest cells are adjacent to the mesodermal core and not to the vessels.
Expression was detected in neural crest cells in the first arch from rhombomere 4. The labeled neural crest cells are adjacent to the mesodermal core and not to the vessels.
Expression was detected in the dorsal half but was absent in the ventral half of the basal plate in the caudal spinal cord. The expression pattern was reiterated along the entire anterior-posterior axis.
Laminin-1 was discontinuously distributed along the basal side of the coelomic epithelium, whereas the basement membrane components are thick and continuous under the mesonephros. Laminin-1 is also deposited on the basal side of the mesonephric tubules.
Expression in the prevertebrae and intervertebral disks was detected, the transcripts being localized around the chondrification centra and at the junction between the ribs and the prevertebrae, and no signal being detected in the chondrification centra.
Expression was detected in the prevertebrae of the entire axial skeleton. Expression was also restricted to a portion of the sclerotome that will give rise to the intervertebral disc.
Expression was detected in the prevertebral column and increased in abundance starting at the level of prevertebra 3, was strongest between prevertebra 6 - 14, and then decreased posteriorly.
Expression was detected in the prevertebral column and increased in abundance starting at the level of prevertebra 3, was strongest between prevertebra 6 - 14, and then decreased posteriorly. Expression pattern was indistinguishable between +/+ and -/-.
Expression was restricted to the marginal zones of the thoracic intervertebral disc anlagen as normally only observed for the anlagen of the chondrifying vertebral bodies. Less frequently, expressed in a stripe along the plane of bilateral symmetry.
Cell counting analysis of expressing cells, summarized in Fig 7D. Only the ratio of positive cells in the layer VI was shown, since expression was only seen in the layer VI.
Cell counting analysis of expressing cells, summarized in Fig 7D. Highest expression. Only the ratio of positive cells in the layer VI was shown, since expression was only seen in the layer VI.
Cell counting analysis of expressing cells, summarized in Fig 7D. Decreased expression compared with that of P14. Only the ratio of positive cells in the layer VI was shown, since expression was only seen in the layer VI.
Expression was detected from the fibrous cell layer to the bottom of the hypertrophic layer in the condylar cartilage of the mandible. This includes the polymorphic cell layer and the flattened cell layer.
Expression was detected in chondrocytes from the flattened cell layer to the bottom of the hypertrophic layer in the condylar cartilage of the mandible. The bottom of the hypertrophic layers gradually reduced expression.
Expression was detected in the periosteal osteogenic cells surrounding the cartilaginous tissue. Slight expression was noted in the fibrous cell layer to the upper hypertrophic layer but is hardly detectable in the lower half of the hypertrophic layer.
Expression was detected in the chondrocytes from the lower polymorphic cell layer to the bottom of the hypertrophic cell layer of the condylar cartilage, but not in the fibrous cell layer. Slight expression was noted in the periosteal osteogenic cells.
Expression was detected in differentiating neurons along the entire neuraxis but was low or absent in the dividing cells of the ventricular layer and the inner parts of the intermediate layer.
Absent to trace in nerve cells and absent in nerve fiber. Ambiguous: the text indicates it is absent but the table shows a that it was either absent to a trace amount in nerve cells.
Absent to trace in nerve cells and absent in nerve fiber. Ambiguous: the text indicates it is absent but the table shows a that it was either absent to a trace amount in nerve cells.
Expressed in the mesenchyme surrounding the cloacal slit, in the region that will form the genital folds and the genital tubercle. Expression was contiguous between the allantois/umbilicus, hindlimbs, and nascent proctodeum.
Expressed moderately in both epithelium and dermal papilla of elongating awl follicles. Staining was negative in awl placodes and awl hair plugs. Intense expression in the small dermal cell condensations of awl hair plugs.
Strong expression was detected in the central portion of the conditional knockout retina, but decreased Otx2 labeling was noted in the distal portion of the retina in the Rx-deletion region.
Broad expression was detected in the ganglion cell layer. Expression in the ciliary margin of the neural retina disappeared at E15.5. Expressing cells were not detected in the neuroblast layer.
Apoptotic cells were detected in the ganglion cell layer. The number was signficantly increased in the mutant compared with wild type littermates. No apoptotic cells were observed in the neuroblast cell layer.
Expression was detected in retinal ganglion cells in the neuroblast layer and the ganglion cell layer. However, the density of retinal ganglion cells was significantly decreased in the mutant compared with wild type littermates.
Expression was detected in the epithelium of segment II of the caput epididymis. Expression was detected in all cells, but prominently higher expression was observed in the narrow cells.
Expression was detected in the epithelium of segment III of the caput epididymis. Expression was weaker than that observed in segment II, but the levels in narrow cells and basal cells appeared unchanged.
Patchy staining along the basal surface of the neural tube. Staining around the otocyst and on the ventrolateral aspect of the adjacent (rhombencephalic) neural tube increased in intensity. The strongest reaction was found between the two structures.
Expression was detected in the dorsal part of the otocyst extending toward the rostral and ventral regions. Expression level was low compared to staining observed in the optic vesicle and the nasal region.
Expression was detected in two domains in the otocyst, one in the dorsomedial region corresponding to the vestibular sensory region adjacent to the endolymphatic duct primordium, and another in the ventral region of the otic epithelium.
The posterior expression domain was downregulated in most, but not all, mutant embryos. The lateral domain was still detectable, but its position was different and was wider along the dorso-ventral axis in the mutant compared to the wild type.
The posterior expression domain was downregulated in most, but not all, mutant embryos. The lateral domain was still detectable, but its position was different and was wider along the dorso-ventral axis in the mutant compared to the wild type.
Expression was detected in the ventral-most area of the otic vesicle. There was no difference in expression level compared to wild type, but the proportion of negative epithelium had decreased signficantly.
Expression was detected only in the otic vesicle. Some cells on the dorsolateral edge may have been unlabeled. No obvious label was observed in the adjacent ectoderm and neural tissue.
There is continuity between cells labeled in the anterolateral epithelium of the otocyst, the adjacent mesenchyme, and the ectoderm. Expression was detected in the vestibular atrium and cochlear pouch.
Expression was detected in the vestibular atrium and cochlear pouch. Expression labeled only one side of the developing cochlear duct, along the posterior/medial edge of the duct, from which the cochlear sensory epithelium develops.
Expression was detected in the vestibular atrium and cochlear pouch. Expression labeled only one side of the developing cochlear duct, along the posterior/medial edge of the duct, from which the cochlear sensory epithelium develops.
Expression was detected in the blood islands of the yolk sac and in the heart and the aorta. Expression is increased in the extraembryonic yolk sac mesoderm and is high in aggregates of cells on the amnion.
The vascular morphology varies in mutants compared to wild type. In those with the bulbous allantois, the vasculature was less severely affected, and the structures like dorsal aortae and intersomitic vessels are visible, albeit hypomorphic.
Expression in the tips was as in wild type in 12/26 cases, bilateral but with unequal staining in 5/26 cases and unilateral in 5/26 cases. Expression in the ureter tips was weak or not detected in 4/26 cases.
Expression in the tips was as in wild type in 12/26 cases, bilateral but with unequal staining in 5/26 cases and unilateral in 5/26 cases. Expression in the ureter tips was weak or not detected in 4/26 cases.
Signals were detected in the spiral limbus, spiral ligament, and stria vascularis in a pattern indicative of sectioned blood vessels. Signal was also detected in spiral vessel, a blood vessel below the organ of Corti.
Expression was detected in hair cells in the apex of the cochlear duct. Staining showed a higher density of hair cells in the apical turn of Prdm16 null cochlea.
Expression was abundant in proliferative epithelia at the base of the crypts and is absent in the nonproliferating differentiated epithelia that have migrated up from the crypt base to the luminal surface.
Expression was detected approximately 100 um layer of mesenchyme underneath the ectoderm in all regions of the limb bud. Expression co-localized with proliferation (BrdU staining). Chondrogenic differentiation (Sox9 immunostaining) was mutually exclusive with proliferation and reporter expression.
Expression in the zeugopod was relatively weak but had a domain in the posterior portion with a clear boundary at the middle of the limb bud. Expression was detected in the presumptive fibula region.
Expression in the zeugopod was relatively weak but had a domain in the posterior portion with a clear boundary at the middle of the limb bud. Expression was detected in the presumptive fibula region.
Expression in the zeugopod was relatively weak but had a domain in the posterior portion with a clear boundary at the middle of the limb bud. Expression was detected in the presumptive fibula region.
Expression was detected in the lens fiber cells beneath the anterior epithelium but was absent from a band of cortical secondary fiber cells. The nuclear fibers and the organelle-free zone at the center of the lens had no staining.
Expression was detected in inner cortical fiber cells that had recently detached from the lens epithelium. Expression was detected in fiber cells immediately prior to denucleation. Immunofluorescence was distributed throughout the cytoplasm.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
