Expression was weak in the ventral thalamus and more pronounced in the ependymal layer of the dorsal thalamus. There was a sharp border of signal at the sulcus limitans.
Expression was detected in ventral thalamus. The caudal limit was around the zona limitans intrathalamica; the ventral limit was at the alar/basal boundary. Both limits were sharply delineated. Dorsal thalamus was negative.
Immunopositive fibers extend from the diencephalon toward the ventral surface of the pallidum. The bundle connects the dorsal thalamus and the basal-marginal telencephalon and was not detected in wild type.
Expression was detected in a lateral-medial band within the dorsal thalamus. Expressing cells were largely distinct from cells expressing Efna5. Co-localization with Epha7 was noted in discrete portions of the positive expression domain.
Crabp1 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
Hoxa3 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
Prx1 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fashion to Pax3.
Prx2 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
c-met was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
Expression was detected in the anlage of the aortico-pulmonary septum (APS). In the region of the future APS, underneath the immunopositive cells, an area of condensed mesenchyme is negative for staining.
There is a reduction in the number of positive cells and decreased staining intensity in this mutant compared to wild-type. Expression was restricted to the nucleus in a few cells.
Expression was limited to a domain that encompasses parts of adjacent areas of both the lesser epithelial ridge and greater epithelial ridge. Immunoreactivity was more intense in the lesser epithelial ridge.
Expression was detected in neural crest cells in the rostral and caudal halves of the somites. Fewer neural crest cells at the presumptive borders between somites maintained a pseudosegmental quality to the migration pattern in the mutant.
Strong staining in the medial region of the limb bud was seen, together with signals in the proximalmost anterior and posterior mesenchyme and in the distal anterior subridge mesenchyme.
Expression was detected in the ectoderm on the ventral surface of the limb bud at the junction of the bud and the flank and the surface ectoderm on the dorsal aspect of the limb adjacent to the flank.
Expression was detected in the ectoderm on the ventral surface of the limb bud at the junction of the bud and the flank and the surface ectoderm on the dorsal aspect of the limb adjacent to the flank.
Expression was detected in the ventral ectoderm. The overall anterior-posterior length of the expression domain did not fade anteriorly at E12.0 (in contrast to Fgf8 - see assay MGI:5439574).
Expression is present throughout the prosomere 2 ventricular zone, with expression extending into a region of the postmitotic mantle zone. It was not present in the Rim/pTh-R/Zli-shell or the Zli.
Expression is present throughout the prosomere 2 ventricular zone, with expression extending into a region of the postmitotic mantle zone. It was not present in the Rim/pTh-R/Zli-shell or the Zli.
Expression is present throughout the prosomere 2 ventricular zone, with expression extending into a region of the postmitotic mantle zone. It was not present in the Rim/pTh-R/Zli-shell or the Zli.
Expression is present throughout the prosomere 2 ventricular zone, with expression extending into a region of the postmitotic mantle zone. It was not present in the Rim/pTh-R/Zli-shell or the Zli.
Expression is present throughout the prosomere 2 ventricular zone, with expression extending into a region of the postmitotic mantle zone. It was not present in the Rim/pTh-R/Zli-shell or the Zli.
Expression was detected in the muscle fibers and the sarcolemma as well as around the muscle fiber nuclei in a striped pattern. Expression was localized in the adjoining area of the z-disc close to Desmin.
Expression was detected in the muscle fibers and the sarcolemma as well as around the muscle fiber nuclei in a striped pattern. Expression was localized in the adjoining area of the z-disc close to Desmin.
Expression levels are higher in testis than ovary. In the testis prominent expression was evident in the testis cords with background levels of expression observed in the adjoining mesonephric tissue.
Expression levels are higher in testis than ovary. In the testis prominent expression was evident in the testis cords with background levels of expression observed in the adjoining mesonephric tissue.
Expression levels are higher in testis than ovary. In the testis prominent expression was evident in the testis cords with background levels of expression observed in the adjoining mesonephric tissue.
Higher magnification was shown in the inset. Expression is in neuroepithelial and granular cells in the ventricular zone and the granular layer of the bulbus and is weak in the mitral layer.
Higher magnification was shown in the inset. Expression is in neuroepithelial and granular cells in the ventricular zone and the granular layer of the bulbus and is weak in the mitral layer.
Expression was detected in the dendrites and axons of mitral cells. There was no co-expression with Gap43 in the olfactory sensory neuron axons in the outer nuclear layer, but partial overlap was noted at the outer nuclear layer border.
Expression was detected in radial glia extending through the dentritic zone to the olfactory nerve layer, where their end feet arranged along the surface in a boundary like pattern.
Positive staining didn't colocalize with trigeminally innervated glomeruli. High expression in glomeruli 1 and 2 but little Calca-like immunoreactivity was present, whereas Calca-immunoreactive fibers entered glomeruli 3 and 4.
Beta-galactosidase positive glomeruli were observed on the medial side of the olfactory bulb. There was an overall ventral shift in the location of the glomeruli in the conditional mutant compared to controls.
A second beta-galactosidase positive glomerulus was observed more caudally on the lateral side of the olfactory bulb in 17/24 mutants. The position of the glomerulus was shifted ventrally in the mutant.
A beta-galactosidase positive glomerulus was observed on the medial side of the olfactory bulb. There were two in 21/26 mutants. The position of the glomerulus was shifted ventrally in the mutant.
A beta-galactosidase positive glomerulus was observed in the ventromedial region of the olfactory bulb. There was a small but significant dorsal shift in the location of the glomerulus in the mutant.
Expression was detected throughout the rostrocaudal extent of R3 and R5 neuroepithelium, most prominent dorsally and at the rhombomere boundaries. Neuroepithelium expression was also detected in a continuum from R1 into the forebrain.
Expression was detected in the anterior two-thirds of the palate mesenchyme. Expression becomes progressively localized to the oral side of the palatal shelf in the posterior region of its domain.
Expression was restricted to mesenchyme immediately adjacent to the overlying Shh domain in the rugae. Broad expression was in a posterior domain of the mesenchyme of the presumptive soft palate.
Expression was detected in an oral-to-nasal gradient in the developing palatal mesenchyme, and levels were increased and extended to the nasal side of the middle portion of the palatal shelves compared with wild type embryos.
Expression was detected in an oral-to-nasal gradient in the developing palatal mesenchyme. Expression was moderately increased in the posterior region of the palatal shelves in Foxf2-/- embryos compared with wild type.
Expression was detected in an oral-to-nasal gradient in the developing palatal mesenchyme, and levels were increased throughout the posterior portion of the palatal shelves compared with wild type embryos.
In the vast majority of embryos examined there was no expression (or there was weak expression in a few scattered cells in the presumptive anterior visceral endoderm and the definitive endoderm regions).
There was weak expression in a few scattered cells in the presumptive anterior visceral endoderm and the definitive endoderm regions (in the central portion of the epiblast and near the distal tip region). (In many cases there was no expression.)
Expression was just discernable in the cranial ridge of mesenchymatous tissue and in the endocardial cushion tissue, but only where this tissue is juxtaposed to the surrounding myocardial cuff.
In pharyngeal arch 2, stapes condensation was demarcated by Sox9 expression in mesenchyme dorsal to the pharyngeal endoderm. At E11.5, expression was downregulated in the center of the stapes condensation region.
In pharyngeal arch 2, stapes condensation was demarcated by Sox9 expression in mesenchyme dorsal to the pharyngeal endoderm. At E11.5, expression was downregulated in the center of the stapes condensation region.
Expression was detected in the Golgi complex of the acinar cells. There was high density labeling of vesicles, and some gold particles were observed in the parallel membranes of Golgi transface.
The anterior expression boundary was shifted in the mutant. Expression was either very weak or lost in prevertebra 8. The most rostral strong expression was detected in prevertebra 9.
Trp63+Krt5+ cells were similar as in the control. There was a notable ectopic population of Trp63-Krt5+ cells detected in the luminal layer as a part of the thickened tracheal epithelium.
Trp63+Krt5+ cells were similar as in the control. There was a notable ectopic population of Trp63-Krt5+ cells detected in the luminal layer as a part of the thickened tracheal epithelium.
Trp63+Krt5+ cells were similar as in the control. There was a notable ectopic population of Trp63-Krt5+ cells detected in the luminal layer as a part of the thickened tracheal epithelium.
Trp63+Krt5+ cells were similar as in the control. There was a notable ectopic population of Trp63-Krt5+ cells detected in the luminal layer as a part of the thickened tracheal epithelium.
Gsc expression was associated with the mesenchyme around the pharyngeal pouches. The highest level of expression was found at the junction between the 1st and 2nd branchial arch in the region that will go on to form the auditory meatus.
Co-expression with smooth muscle alpha-actin was noted in arterial smooth muscle cells. The relative expression level appeared to be higher in arterial smooth muscle at E14 compared to E10.
Expressed in the intermediate nerve, facial motor root, and proximal part, but was absent in the distal portion of the facial nerve beyond the branch point with the chorda tympani.
Expression was detected in proliferating chondrocytes in the proliferative zone in intrasphenoidal synchondroses. Scattered proliferating chondrocytes were also seen the in reserve zone, but not in the hypertrophic zone.
Expression was detected in proliferating chondrocytes in the proliferative zone in intrasphenoidal synchondroses. Scattered proliferating chondrocytes were also seen the in reserve zone, but not in the hypertrophic zone.
Expression was detected in proliferating chondrocytes in the proliferative zone in intrasphenoidal synchondroses. Proliferating chondrocytes in the reserve zone had decreased by P0. No positive cells were in the hypertrophic zone.
Expression was detected in proliferating chondrocytes in the proliferative zone in spheno-occipital synchondroses. Scattered proliferating chondrocytes were also seen the in reserve zone, but not in the hypertrophic zone.
Expression was detected in proliferating chondrocytes in the proliferative zone in spheno-occipital synchondroses. Scattered proliferating chondrocytes were also seen the in reserve zone, but not in the hypertrophic zone.
Expression was detected in proliferating chondrocytes in the proliferative zone in spheno-occipital synchondroses. Proliferating chondrocytes in the reserve zone had decreased by P0. No positive cells were in the hypertrophic zone.
In the capillary plexus of the yolk sac, the venous plexus occupies a slightly larger fraction of the vascular plexus than the arterial region as compared with heterozygous littermates. Data was quantified in Fig. 5E.
Expression was detected in a rostrodorsal compartment with a decreasing mediolateral gradient, but there was a lack of expression in a caudoventral compartment. Co-staining with Calretinin showed overlap in the lateral rostrodorsal compartment.
A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
A negative inferior colliculus domain was intercalated between the superior colliculus and the preisthmic domains. A subpial positive cell stream seems to invade the superficial inferior colliculus mantle out of the preisthmus.
Expression was found in ther papillary and reticular layers of the derms and all along the border of striated muscle sheets present between the dermis and the subcutaneous tissue.
Expression was detected in back skin. Data was presented in a graph of the number of immunopositive cells. There was no difference between the littermate control and the mutant.
Expression was detected in both the dorsal and ventral pancreatic buds, as well as in the intervening gut endoderm. Expression was detected in the majority of the pancreatic primordial cells, including the early glucagon-producing cells.
Expression was detected in the dorsal and ventral pancreatic buds as well as in the intervening gut endoderm. Expression was absent or near absent in the pancreas-associated mesenchyme and in a substantial number of cells that coexpressed glucagon.
Expression is easily detected in several cells in the epithelial lining of the cap-like dorsal anlage of the pancreas. Additional expression was occasionally seen in the wall of the ducts. It is not seen elsewhere in the embryo.
Expression is easily detected in several cells in the epithelial lining of the cap-like dorsal anlage of the pancreas. Additional expression was occasionally seen in the wall of the ducts. It is not seen elsewhere in the embryo.
Expressed in the purkinje cell layer. Highly expressed in all lobules in the vermis and hemisphere. Expression was less intense in the nodulus (vermal lobule X), and the bottom of the fissure between lobules III and IV.
Gold labeling was detected along microfibrils in neonatal elastic cartilage irrespective of the presence or absence of amorphous elastin. No labeling was detected on collagen fibers or amorphous elastin.
Expression was detected in a broad domain in most of the diaphysis. There is decreased expression in the most mature hypertrophic chondrocytes at the center of the hypertrophic zone.
Expression was detected in the periosteum of the shaft, in hypertrophic cartilage of the growth plate and within the trabecular bone. There was no staining at or near the articular region.
Expression was detected predominantly in mature and terminally differentiated hypertrophic chondrocytes, the perichondrium and the primary spongiosa. Maximal expression in the hypertrophic chondrocytes was seen at E15.5 and E16.5.
Two separate and distinct chondrocyte populations were noted. The upper proliferative chondrocytes (higher mag in 4E), and the subarticular chondrocytes (higher mag in 4F). The periosteum was stained, but the primary spongiosa was not.
Strong immunostaining was detected in the diaphysis, but there was less staining toward the epiphysis, and none at the epiphyseal end. No immunostaining was detected in the marginal area of the metaphysis and epiphysis.
There was no obvious change in Skil expression levels in prehypertrophic and hypertrophic chondrocytes in the double mutant compared with control. However, expression was increased in the proliferative zone.
There was no obvious change in Ski expression levels in prehypertrophic and hypertrophic chondrocytes in the double mutant compared with control. However, expression was increased in the proliferative zone.
Expression was largely confined to the medial tibial plateau. The lateral portion of the plateau exhibited ony a few scattered expressing cells. The anterior and posterior contours were essentially negative.
Expression was largely confined to the medial tibial plateau. The lateral portion of the plateau exhibited ony a few scattered expressing cells. The anterior and posterior contours were essentially negative.
Expression was concentrated in the white matter at the level of all major fiber tracts, from the olfactory bulb and the corpus callosum in the forebrain to the spinal cord.
Expression signal was distributed at cell-cell contact of the inner and outer dental epithelium of the lingual side, whereas in the labial side the labeling accumulated at the basal pole of the cells.
Expression signal was distributed at cell-cell contact of the inner and outer dental epithelium of the lingual side, whereas in the labial side the labeling accumulated at the basal pole of the cells.
Expression was detected in the dental epithelium, but absent in the basement membrane and epithelial cells in the basal layer. At the tip of the dental epithelium, the staining was pronounced.
Expressed in the myocytes of the trabecular network and the compact free wall of the left ventricle, but no immunoreactivity was detected in the right ventricle. No expression in the interventricular septum.
By E12.5, there were well-formed primary branches in the pancreas, and Cpa1 expression was largely restricted to the branching tips. At all embryonic stages examined, Cpa1-positive cells do not express mature endocrine hormones.
By E12.5, there were well-formed primary branches in the pancreas, and Cpa1 expression was largely restricted to the branching tips. At all embryonic stages examined, Cpa1-positive cells do not co-express the early endocrine progenitor marker, Neurog3.
Expression was detected in the newly formed branches of the dorsal pancreas at E12.5. Expression was downregulated in the 'cleft' region between future branches. Expression did not overlap with Neurog3 staining in the trunk.
Weak expression was detected in the newly formed branches of the dorsal pancreas at E12.5, where it co-expressed Cpa1. Expression was downregulated in the 'cleft' region between future branches.
Weak expression was detected in the newly formed branches of the dorsal pancreas at E12.5, where it co-expressed Cpa1. Expression was downregulated in the 'cleft' region between future branches.
Crabp1 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
Hoxa3 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
