Prx1 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fashion to Pax3.
Prx2 was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
c-met was expressed in a stream of cells that appeared to transverse the third, fourth and developing sixth branchial arches, entering the aortic sac and outflow tract in a similar fasion to Pax3.
Expressed in the interphotoreceptor matrix around the outer segments. A sharp reduction in expression at the border between outer and inner segments. There was no significant variation in expression distribution or strength.
Expressed in the interphotoreceptor matrix around the outer segments. A sharp reduction in expression at the border between outer and inner segments. There was no significant variation in expression distribution or strength.
Expression was detected in neurons as they migrated out of the ganglionic eminence and entering the cortex. However, the medial pallium was not reached by the neurons in the mutants.
Expression was absent from the apical cells, and thus from the surface mitoses of the ventricular zone, but was present in a band of cells that includes the basal, nonsurface mitotic precursors of the subventricular zone.
Expression was detected throughout the rostrocaudal extent of R3 and R5 neuroepithelium, most prominent dorsally and at the rhombomere boundaries. Neuroepithelium expression was also detected in a continuum from R1 into the forebrain.
Expression extended over the caudal two thirds of the midbrain. Expression was not throughout the thickness of the neuroepithelium, but in single neuroblasts on the pial surface of the midbrain.
Staining in the telencephalic vesicles was considered as an artefact since it could also be observed in control embryos either hybridized with a sense mouse PN-1 riboprobe or without a riboprobe.
Primary somatosensory cortex was shown. Dramatically increased density (approximately a 50% increase), and the distribution markedly shifted toward the superficial layer. Immunofluorescent signal was also much stronger compared with the control.
Primary somatosensory cortex was shown. Dramatically increased density (approximately a 90% increase), and the distribution markedly shifted toward the superficial layer. Immunofluorescent signal was also much stronger compared with the control.
Primary somatosensory cortex was shown. Dramatically increased density (approximately a 50% increase), and the distribution markedly shifted toward the superficial layer. Immunofluorescent signal was also much stronger compared with the control.
Primary somatosensory cortex was shown. Dramatically increased density (approximately a 50% increase), and the distribution markedly shifted toward the superficial layer. Immunofluorescent signal was also much stronger compared with the control.
Primary somatosensory cortex was shown. Increased density by around 50%. The distribution shifted toward the superficial position because the fold change was more prominent in the superficial region (see Fig 5C).
Immunopositive neurons were present in the mutant, but they were significantly fewer than in the wild type, with a much weaker density and a diffuse, unstructured distribution of signal. This was particularly prominent for the double labeled neurons.
Immunopositive neurons were present in the mutant, but they were significantly fewer than in the wild type, with a much weaker density and a diffuse, unstructured distribution of signal. This was particularly prominent for the double labeled neurons.
Expression in the brainstem was predominant in the pre-Botzinger complex, the nucleus Ambiguus, and the facial motor nucleus. Expression was much lower if barely detectable in the retrotrapezoid nucleus/parafacial respiratory group.
The number of Sst/Pax2 coexpressing neurons was severely reduced in the mutant pre-Botzinger complex. However, the overall number of Pax2-positive neurons in the same region was only marginally affected.
Expressed in the red nucleus and ipsilateral tectopontine tract, but not the the decussating fibers of the tectospinal tract. Reduced extent of the red nucleus and ventral tegmental decussation, and the rubrospinal tract was nearly absent.
Lmx1b is a marker for dILB glutamatergic neuronal cell population. In the mutant embryos, ectopic expression of Kirrel1 in most superficial layers was extensively present in the Lmx1b-positive glutamatergic cells.
Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles. Phenotypic abnormalities were noted.
Expression was detected in the vagus nerve at the gastroesophageal junction and as vagal nerve fibers extended into the stomach and then branched into smaller bundles. Phenotypic abnormalities were noted.
The pattern of labelling does not differ greatly from the previous stage. The number of neurofilament-positive cells or fibers appears increased. Description of the pattern was summarized in Table 2.
Expression in the ventricular zone throughout the CNS, although at different levels in different regions. In many regions, expression was not seen in all cells of the ventricular zone.
Observed both Fgf5-expressing (likely definitive endoderm cells) and non-expressing cells in the endodermal cell layer. Distally, Fgf5 mRNA was detected a levels similar to those in adjacent embryonic ectoderm; proximally, no Fgf5 mRNA was detectable
In contrast to expression at P0, labeling was no longer present throughout the entire suprachiasmatic nucleus; instead, expression was restricted to only the outer 71% of the suprachiasmatic nucleus.
Expression was detected predominantly in the more extensively condensed mesenchyme of the ventral half of the otocyst. Low levels of expression were detected in the aggregation center in the dorsomedial aspect.
The overall expression level of mutant embryos was reduced compared to wild type. This reduction in expression was most readily apparent in the posteriormost regions of the mutant in the neural folds and lateral mesoderm.
Expressing cells were observed from the first somites to the level of the hindlimb buds in the same locations as cells expressing Kit mRNA. No expression was detected in the lateral mesenchyme.
Expression was detected in a longitudinal band of mesenchyme in the lateral region of the trunk. This may correspond to the Wolffian ridge, from which the limb buds originally grow out.
Expression was detected in the condensing periotic mesenchyme that is dorsal and lateral to the horizontal semicircular duct and to the mesenchyme that is lateral to developing vestibular structures.
Expression was observed in the mesenchyme surrounding the vestibular system and the cochlea and the cartilage primordium of the temporal bone, but at a lower level than in the otic epithelia.
Expression was observed in the mesenchyme surrounding the vestibular system and the cochlea and the cartilage primordium of the temporal bone, but at a lower level than in the otic epithelia.
Expression was detected in the cytoplasmic face of the oolemma in 100% of oocytes. In the cytocortex, the microvillous domain was stained, but staining was absent or poor in the smooth membrane domain overlying the metaphase II spindle.
Expression was detected in the oocyte cortex in 100% of the oocytes. Staining was not detected in most cumulus cells except within the corona radiata weakly at the site of contact with the oocyte.
Expression was detected in the oocyte cortex in 100% of the oocytes. Staining was not detected in most cumulus cells except within the corona radiata weakly at the site of contact with the oocyte.
The intensity of Nestin immunostaining was unchanged compared with wild type. There was a reduction in thickness of the layer, which indicated a decrease in the total number of radial glial cells.
The intensity of Nestin immunostaining was unchanged compared with wild type. There was a reduction in thickness of the layer, which indicated a decrease in the total number of radial glial cells.
Expressed in the dermal lymphatic sprouts, which were dilated and failed to migrate toward the midline of the embryo. There was an increased number of cells in lymphatic vessels.
Expression was detected in a subset of unidentified cells in the dermal mesenchyme of the skin; the cells were distributed among those tissues that will form superficial muscle layers and connective tissue elements of the dermis.
Expression was detected in the dermal mesenchyme. There were local concentrations of cells along the abdominal wall at the level of the pelvis, where the hindlimbs come off the body wall.
Expression domain in the lateral aspects of the anterior neural plate. The medial region of the anterior neural plate (which normally does not express Otx1) is absent in the embryo, and now expression was seen across the midline.
Expression was detected in a stripe at the isthmus and dorsally it is expressed from the isthmus to part of the dorsal thalamus (p2 diencephalon). A negative region was noted in the anterior diencephalon.
Expression was more anteriorly located in the double heterozygote, but the stripe of expression was nearly unchanged. The negative region in the roof of the anterior diencephalon contained a line of expressing cells.
Expression was restricted to the ventral midline in most of the brain, with the exception of the rostral diencephalon where expression skirts the presumptive hypothalamic region, the paramedian plate.
Expression was detected in the Telenecephalic Vesicles, dorsal spinal cord and the laterovertebral mesenchyme. The latervertebral mesenchyme is defined as the cells located between the prevertebrae and the dorsal root ganglia.
In rostral sections of E12 embryos, where the floor plate was present, expression in the heterozygote was the same as in wild-type embryos. Aberrant expression was evident in caudal sections of heterozygous embryos where the floor plate was absent.
Expression was detected in the sensory routes, in the motor neuron columns and in a narrow region below the floor plate running between the two motor neuron columns in the caudal spinal cord.
In rostral sections, where the floor plate was present, expression was detected as in wild type. In caudal sections, where the floor plate was absent, aberrant expression was detected.
Rostral to the region lacking a floor plate, expression was detected in the enlarged intermediate zone. It was absent in cells that give rise to the intermediate central gray region and from presumptive motor neuroblasts in the ventrolateral basal plate.
Expression was detected in the sensory routes projecting laterally from the dorsal alar plate, in the motor neuron columns and in a narrow region below the floor plate running between the two motor neuron columns in the caudal spinal cord.
Weak expression in the spendymal layer of the alar plate, but the strong signal in dorsalmost cells (seen in wild-type) was absent, and there was a dorsalmost region where no expression was detected.
Expression was not detected in the dorsalmost region. In other regions of the presumptive alar plate, expression was significantly stronger than in wild type embryos. The ventralmost region negative for expression appeared to be enlarged.
Transcripts showed a marked anterior-to-posterior fall in abundance over the anterior region of the spinal cord. This fall in abundance was anterior to prevertebra 1 in dorsal parts of the spinal cord but posterior to prevertebra 5 in ventral parts.
Expression had an approx. anterior border in the spinal cord at prevertebra 19 level. High levels of expression were maintained posteriorly through anterior lumbar segments and then decreased at more caudal levels. Expression was unaltered in mutants.
In mutants, in the absence of the floor plate, expression was very intense at the ventral midline. In some mutant embryos, expression was present at both the dorsal and ventral midline.
The specimen was from the level of the cervical vertebra 8. At this level, expression was detected in somatic motor neurons (but not all) in the lateral motor column of the heterozygous mutant.
The specimen was from the level of the cervical vertebra 8. At this level, expression was detected in somatic motor neurons (but not all) in the medial motor column of the heterozygous mutant.
Expression was detected in the soma of motor neurons of the lower cervical spinal cord. Axon tracts of the white matter and the dorsal and ventral roots continue to show expression.
Expression was detected in the soma of motor neurons of the lower thoracic spinal cord. Axon tracts of the white matter and the dorsal and ventral roots continue to show expression.
Expression was primarily confined to the midline, extending along the entire dorsoventral axis of the midline gray matter. Signal observed throughout the spinal cord gray matter was not significantly above background.
Expression was stronger in the dorsal part. A few strong stained regions in the ventral most part, which may correspond to the location of motoneuron columns. No expression in the peripheral layer.
Expression was detected in the dorsal spinal cord in a region lateral to the roof plate, which was further reduced in size compared to E11.5, and in three ventral clusters.
Expression was detected in the intermediate and ventromedial parts of the spinal cord, and in the posterior median septum. The dorsal horn only had expressing cells in the basal portion.
Expression was detected in V2b interneurons. There was some co-expression with Pax6 positive interneurons settled in the ventral spinal cord and not when they migrated out of the ventricular zone.
Expression was detected in interneurons. There was some co-expression with Gata3 positive V2b interneurons. Only the Pax6 interneurons settled in the ventral spinal cord coexpressed Gata3, and not the Pax6 interneurons when they migrate out of the ventricular zone.
Expression was detected in V2b interneurons. There was some co-expression with Pax6 positive interneurons settled in the ventral spinal cord and not when they migrated out of the ventricular zone.
Expression was detected in interneurons. There was some co-expression with Pax2 positive ventral V0 interneurons and with Gata3 positive V2b interneurons. There were also Pax6 positive interneurons that do not express either Pax2 or Gata3.
Expression was strong in the medial part of the mantle zone, but the intermediate zone and the most ventral part of the spinal cord were not labeled. Expression was in postmitotic neurons.
Expression is in commissural axons that extended ventrally, similar to wild-type embryos but in this mutant immunoreactivity was reduced in the medial ventral funiculus to only 32.3% of the wild-type levels.
Expression is in commissural axons that extended ventrally, similar to wild-type embryos but in this mutant immunoreactivity was reduced in the medial ventral funiculus to only 32.3% of the wild-type levels.
Expression was detected in a dorsal position in a region lateral to the roof plate. Compared to adjacent sections, there is a partial overlap of the expression pattern with Wnt1 and Lhx2 in the caudal region.
Expression was detected in a dorsal position in a region lateral to the roof plate. Compared to adjacent sections, there is a partial overlap of the expression pattern with Wnt1, but not Lhx2, in the cervical region.
Expression was detected in a subset of dorsal commissural neurons. Compared to staining in an adjacent section, there was overlap of Lhx2 and Atoh1 expression domains. There were Lhx2 only positive cells in the lateral region.
Expression was widely distributed in the ventral spinal cord. The only regions that are devoid of staining were the neuroepithelium and the ventrolateral spinal cord, where preganglionic and somatic motor neurons are located.
Expression was detected in precrossing axonal domains and at the midline. Its expression is rapidly switched off after midline crossing because it was not seen at all in ventral fiber tracts.
Expression was detected in precrossing axonal domains and at the midline. Its expression is rapidly switched off after midline crossing because it was not seen at all in ventral fiber tracts.
From the region near the otocyst a rod of expressing cells was seen, running down from the otic region parallel to the branchial artery towards the distal tip of the second arch.
Expression was detected neurons in the external muscle layers of the rectum. Immunopositive neurons were found along the entire colon in the mutant, and densities of myenteric neurons were not significantly different from wild type littermates.
Expression was detected neurons in the external muscle layers of the rectum. Immunopositive neurons were found along the entire colon in the mutant, and densities of myenteric neurons were not significantly different from wild type littermates.
Expression was detected neurons in the external muscle layers of the rectum. Immunopositive neurons were found along the entire colon in the mutant, and densities of myenteric neurons were not significantly different from wild type littermates.
Expression was detected neurons in the external muscle layers of the rectum. Immunopositive neurons were found along the entire colon in the mutant, and densities of myenteric neurons were not significantly different from wild type littermates.
Expression was detected neurons in the external muscle layers of the rectum. Immunopositive neurons were found along the entire colon in the mutant, and densities of myenteric neurons were not significantly different from wild type littermates.
Expression was detected neurons in the external muscle layers of the rectum. Immunopositive neurons were found along the entire colon in the mutant, and densities of myenteric neurons were not significantly different from wild type littermates.
Immunogold particles are localized on the cytoplasmic side immediately below the paranodal loop membranes. Pairs of immunogold particles are often observed on apposing membranes. No immunogold was detected in the axo-glial junctions of paranodes.
Immunogold particles are localized on the cytoplasmic side immediately below the paranodal loop membranes. Pairs of immunogold particles are often observed on apposing membranes. No immunogold was detected in the axo-glial junctions of paranodes.
Expression was strong within newly forming epithelial somites, but only in the rostral half. Restriction of expression to the rostral half somites was clearest in the two most recently formed somites and was less detectable in more rostral somites.
Expression was in the somitic sclerotome. Expression was confined to perichondrium and osteoblasts in the anterior part of the sclerotome-derived vertebral column, while the centers of ossification were negative.
The overall expression level of mutant embryos was reduced compared to wild type. This reduction in expression was most readily apparent in the posteriormost regions of the mutant in the neural folds and lateral mesoderm.
Pax-3 expression was evenly distributed over newly formed somites, whereas at later stages of somitic development the Pax-3 signal was confined to the most caudal and ventrolateral edge in each somite.
Pax-3 expression was evenly distributed over newly formed somites, whereas at later stages of somitic development the Pax-3 signal was confined to the most caudal and ventrolateral edge in each somite.
Pax-3 signal was clustered in the ventrolateral bud appearing in individual somites of the trunk. A clearly weaker expression domain was observed more dorsomedially in two parallel stripes along the anterior and posterior borders of each somite.
The somitic area normally labelled by Pax-3 appeared much shorter and disorganized. The edges of the somites delineated by the Pax-3 signal appeared irregular. The Pax-3 expression domain was slightly shifted ventrally, away from the neural tube.
Expression was detected in all somites except the two to three caudal pairs that were the most recent to segregate. Expression was restricted to the most dorsal part of the somites.
Expression was detected in all somites except the two to three caudal pairs that were the most recent to segregate. Expression was restricted to the most dorsal part of the somites.
Expression was detected in all somites except the two to three caudal pairs that were the most recent to segregate. Expression was restricted to the most dorsal part of the somites. Expression detected in the otic vesicle.
Authors state that Pax3 expression patterns (in the future dermomyotome) revealed that paraxial mesenchyme in the thoracic region becomes properly segmented. However, in dorsal somitic regions, an abnormal, flame-like pattern of expression was observed.
Expression in the earliest somites occurred throughout the inner edge of the somitic epithelium and was stronger dorsally. In slightly more mature somites, expression had decreased throughout the epithelium and increased at the somitic lips.
Expression in the earliest somites occurred throughout the inner edge of the somitic epithelium and was stronger dorsally. In slightly more mature somites, expression had decreased throughout the epithelium and increased at the somitic lips.
