Rostrally, expression in neural crest cells in somites were aggregating at future dorsal root ganglia and sypathetic ganglia. Caudally, expression was in neural crest cells migrating in orderly streams through the rostral half of the somites.
Expression was detected in neural crest cells in the rostral and caudal halves of the somites. Fewer neural crest cells at the presumptive borders between somites maintained a pseudosegmental quality to the migration pattern in the mutant.
Staining was present in the cytoplasm. There was intense staining encircling the cell borders and margins; this was most intense near unopposed cell membrances, compared to cell membranes near adjacent cells.
Co-localization with Padi6 was observed at the non-opposed cortex regions of 2-cell embryos with less co-localization being observed throughout the cytoplasm. Nlrp5 (Mater) staining penetrated deeper into the cytoplasm compared with Padi6.
Co-localization with Nlrp5 (Mater) was observed at the non-opposed cortex regions of 2-cell embryos with less co-localization being observed throughout the cytoplasm. Nlrp5 (Mater) staining penetrated deeper into the cytoplasm compared with Padi6.
Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing hip, tibia and fibula.
Expression was detected equally and prominently in both external and internal layers of smooth muscle in the intestinal wall. Expression overlapped but was not identical with smooth muscle alpha-actin.
Expression was detected in both layers of smooth muscle in the intestinal wall but was predominantly in the internal layer. Expression overlapped with Ppp1r14a (Cpi17) but was not identical.
Laminin-1 was discontinuously distributed along the basal side of the coelomic epithelium, whereas the basement membrane components are thick and continuous under the mesonephros. Laminin-1 is also deposited on the basal side of the mesonephric tubules.
Expression was detected scattered throughout most of the gonad, but the anterior and posterior tips were devoid of signal. Expression was decreased in the XY gonad compared to the XX gonad.
The pattern of labelling does not differ greatly from the previous stage. The number of neurofilament-positive cells or fibers appears increased. Description of the pattern was summarized in Table 2.
In the cortex, expression was detected in the nuclei of epithelial cells constructing the convoluted and straight proximal tubules. Immunohistochemistry showed a positive reaction in the neck of the proximal tubule.
In the cortex, expression was detected in the nuclei of epithelial cells constructing the convoluted and straight proximal tubules. In situ hybridization did not detect a signal in the neck of the proximal tubule.
At E15.5, expression was found surrounding the entry zone, where the olfactory nerve enters the olfactory bulb. However, there was no expression in olfactory ensheathing cells within the entry zone.
Expression was detected at low levels in the olfactory ensheathing cells surrounding the olfactory nerve axons as it passes through the cribriform plate. There was some overlap with perlecan.
A few regions were positive for both OCT3/4 and GATA6, indicating that the patterning of epiblast and visceral endoderm was impaired. Positive cells did not exhibit epithelial structure but they appeared as a clump.
Expression was restricted to very limited concentric zones of the femur perichondrium. The labeled areas were located slightly distally to the front of hypertrophic chondrocytes and overlying the cartilage 'elongation zone'.
Wnt5A showed a dramatic increase in expression throughout the uterine stroma, with expression increasing in stromal cells located with increasing proximity to the myometrial layer. No elevated expression was observed within the endometrial epithelium.
Expression was detected in the inter-implantation sites. Expression was detected in basal laminae associated with the uterine lumen, glandular epithelial cells, endothelial cells of vascular elements and the mesothelial covering of the uterus.
Weak signal was detected in myometrial smooth muscle, in some uterine stromal cells distant from the site of implantation, and adjacent to the uterine cavity. The uterine surface epithelium was negative.
Expression was found in a continuous territory up to the choroid plexus of the fourth ventricle with no gap of expression due to the loss of tissue in the mutant.
Pax-6 expression was confirmed to be in a longitudinal band through the alar plate from the region of the optic stalk to the border between the diencephalon and mesencephalon.
At E14.5 expression in the brain persisted at higher levels in every domain found at E12.5. Expression was broader and more intense in the thickened regions of the subventricular zone adjacent to the neuroepithelium.
Expression was detected in the periventricular zone and neuroepithelium. Expression was also in the intermediate zone and in the ganglionic eminences. Staining was distinct from the pattern of Numb staining.
Expression was ubiquitous along the neuraxis with the highest amounts being evident in the ventricular and subventricular zone, differentiating fields and the median eminence. Expression was also detected in the meningeal precursors.
Expression was detected in all parts of the brain with the exception of the forebrain, where only weak signals were seen. Expression was restricted to postmitotic neurons that had left the ventricular zone.
Expression was detected in all parts of the brain with the exception of the forebrain, where only weak signals were seen. Expression was restricted to postmitotic neurons that had left the ventricular zone.
Expression was detected in all parts of the brain with the exception of the forebrain, where only weak signals were seen. Expression was restricted to postmitotic neurons that had left the ventricular zone.
Expression was detected in all parts of the brain with the exception of the forebrain, where only weak signals were seen. Expression was restricted to postmitotic neurons that had left the ventricular zone.
The medial habenular axons of the fasciculus retroflexus striking misdirection toward rostral domains were seen; no single axon was detected toward their caudal target. The misrouted tract seemed related to an enlarged A13 dopaminergic population located in the prethalamic territory.
In anterior territories of the cerebellar ventricular zone, a high transcript level was detected in a domain located close to midline. Expression was weaker more laterally. There was overlap with Ngn2 (Neurog2) in posterior territories.
In anterior territories of the cerebellar ventricular zone, a high transcript level was detected in a domain located close to midline. Expression was weaker more laterally. There was overlap with Ngn2 (Neurog2) in posterior territories.
In anterior territories of the cerebellar ventricular zone, a high transcript level was detected in a domain located close to midline. Expression was weaker more laterally. There was overlap with Ngn2 (Neurog2) in posterior territories.
In anterior territories of the cerebellar ventricular zone, a high transcript level was detected in a domain located close to midline. Expression was weaker more laterally. There was overlap with Ngn2 (Neurog2) in posterior territories.
Expression was detected in the ventricular zone. There was a gap in the expression domain that coincided with the Pax2 domain in an adjacent section. More rostrally, expression was adjacent to a weaker Pax2 domain.
Expression was detected in the subventricular zone. The domain was adjacent to a gap in the Ngn1 (Neurog1) signal (arrow). Weaker expression more rostrally was adjacent to Ngn1 (Neurog1) signal (arrowhead).
Expression was detected in the rostral rhombic lip migratory stream cells. Some fourth ventricle roof plate cells lost their identity and migrated into the cerebellar anlage in the mutant.
Expression was detected in the rostral rhombic lip migratory stream cells. Some fourth ventricle roof plate cells lost their identity and migrated into the cerebellar anlage in the mutant.
Expression was detected in granule cells. Co-staining revealed that some EdU+ cells populating the dorsal folia expressed low levels of Pax6, suggesting they were postmitotic granule cells that had migrated inward.
Expressed on both the periosteal and endosteal surfaces, at much reduced levels compared to E15.5. Expression was strongest in suture mesenchyme and adjacent bone fronts of both overlapping and abutting sutures.
Expressed on both the periosteal and endosteal surfaces, at much reduced levels compared to E15.5. Expression was strongest in suture mesenchyme and adjacent bone fronts of both overlapping and abutting sutures.
Expression was detected in the cells, which may include neural crest cells, next to the posterior half of the otic vesicle in a domain which extends ventrally next to rhombomere 6.
Expression gradually faded on the lateral side of the head concomitant with the expansion of the calvarial bone into this region. Expression was significantly down-regulated in the cranial mesenchyme except in the meningeal layer.
Expression gradually faded on the lateral side of the head concomitant with the expansion of the calvarial bone into this region. Expression was significantly down-regulated in the cranial mesenchyme except in the meningeal layer.
Expression gradually faded on the lateral side of the head concomitant with the expansion of the calvarial bone into this region. Expression was significantly down-regulated in the cranial mesenchyme except in the meningeal layer.
Expression was restricted to a thin outer layer of cells in the junctional zone. Cells in this region were also positive for trophoblast lineage markers Tpbpa, Prl2c2 and Prl3b1.
Expression was detected on the cell bodies and processes in the subventricular zone of the anterior corner of the lateral ventricle, co-labeling with Nestin. Expression was in neural stem cells (Type B) in the adult subventricular zone.
Expression was detected on the cell bodies and processes in the subventricular zone of the anterior corner of the lateral ventricle, co-labeling with GFAP. Expression was in neural stem cells (Type B) in the adult subventricular zone.
Expression is strong in the center and becomes diffuse toward the periphery. Quantification of the expression domains indicated that expression was more contained, but the differences in the area occupied did not reach statistical significance.
Expression is patchy and more restricted to the small sparse trabeculae. There was a 85% reduction of the expression domain as well as 76% reduction in the number of positive cells compared to controls.
Expression is strong in the center and becomes diffuse toward the periphery. Quantification of the expression domains indicated that expression was more contained, but the differences in the area occupied did not reach statistical significance.
Expression was more prominent at sites of intramembranous ossification. The signal was predominantly in osteoblasts and periosteal cells. Expression was low or undetectable in chondrocytes and other cells surrounding osteogenic cells.
Expression at the anterior tip had spread along the ventral aspect of the genital ridge toward the posterior pole, but there was still an anterior bias in the XX gonad.
Expression was detected throughout the ovary, but absent in the overlying epithelium. Signal was localized to germ cells within germline cysts and to somatic cells in contact with germ cells.
Weak diffuse expression was detected in most cells forming the ovarian structure. Level of expression was higher at E18.5 than at E14.5. Higher magnification images showed specific expression in the epithelial pregranulosa cells associated with the ovarian cysts.
Although some GATA4-positive/p27-positive/Lgr5-GFP-positive cells were also detected in E16.5 ovaries, there was a distinct demarcation between GATA4-positive/p27-negative/Lgr5-GFP-positive cells located in the cortex and the GATA4-positive/p27-positive/Lgr5-GFP-negative cells located in the medulla.
Although some GATA4-positive/p27-positive/Lgr5-GFP-positive cells were also detected in E16.5 ovaries, there was a distinct demarcation between GATA4-positive/p27-negative/Lgr5-GFP-positive cells located in the cortex and the GATA4-positive/p27-positive/Lgr5-GFP-negative cells located in the medulla.
Although some GATA4-positive/p27-positive/Lgr5-GFP-positive cells were also detected in E16.5 ovaries, there was a distinct demarcation between GATA4-positive/p27-negative/Lgr5-GFP-positive cells located in the cortex and the GATA4-positive/p27-positive/Lgr5-GFP-negative cells located in the medulla.
Reduced expression in both the proximal and distal duodenum, compared with the wild type. Expression was uniform when no atresia formed. Expression was restricted to the mesenteric side. Little or no expression within the antimesenteric mesoderm.
Expression in lower cervical and upper thoracic regions of mutants shows a severe reduction in formation of dorsal muscle, but formation of ventral musculature and intercostal muscle was not impaired.
Expression was detected in Henle's layer of the inner root sheath. No expression was in Huxley's layer of the inner root sheath, in the outer root sheath, or in connective tissue sheath.
Expression was detected in Henle's layer of the inner root sheath. No expression was in Huxley's layer of the inner root sheath, in the outer root sheath, or in connective tissue sheath.
Expression was detected in Henle's layer of the inner root sheath. No expression was in Huxley's layer of the inner root sheath, in the outer root sheath, or in connective tissue sheath.
Lbh expression in the ventricular myocardium exhibited a temporary sidedness, which was lost by E12.5. Transcripts localized to the outer proliferative compact layer of the ventricular myocardium and to a much lesser extent in the trabeculae.
Present in the outer retina, including the peripheral margin, in a graded fashion, with higher expression in the dorsal retina compared to the ventral retina. There was no expression in the retinal ganglion cell layer.
Expression was detected in the inner neuroblastic layer and in a subset of cells in the outer neuroblastic layer. The expression level was higher in the inner neuroblastic layer.
Expression was detected in the outer neuroblastic layer and in a subset of cells in the inner neuroblastic layer. The expression level was higher in the outer neuroblastic layer.
Expression was detected in the inner neuroblastic layer and in a subset of cells in the outer neuroblastic layer. The expression level was higher in the inner neuroblastic layer.
Expression was detected in the inner neuroblastic layer of the central retina and in a subset of cells in the outer neuroblastic layer. The expression level was higher in the inner neuroblastic layer.
Expression was detected in the inner neuroblastic layer and in a subset of cells in the outer neuroblastic layer. The expression level was higher in the inner neuroblastic layer.
Expression was detected in the inner neuroblastic layer and in a subset of cells in the outer neuroblastic layer. The expression level was higher in the inner neuroblastic layer.
Expression was detected in the inner neuroblastic layer of the posterior retina and a subset of cells in the outer neuroblastic layer of the posterior retina. The expression level was higher in the inner neuroblastic layer.
Expression was detected in a subset of cells in the outer neuroblastic layer and the scleral inner neuroblastic layer. The expression level was higher in the scleral inner neuroblastic layer.
Expression was detected in all cells of the ventral neural retina. Expression was noted in a high-ventral to low-dorsal gradient and along the nasal/temporal axis with highest expression nasally.
Expression was detected in dividing progenitor cells in the outer neuroblast layer of the retina. Expression was excluded from postmitotic precursors and differentiated cells in the inner neuroblast layer.
Expression was detected in dividing progenitor cells in the outer neuroblast layer of the retina. Much lower levels were noted in postmitotic precursors and differentiated cells in the inner neuroblast layer.
Expression was detected in dividing progenitor cells in the outer neuroblast layer of the retina. Much lower levels were noted in postmitotic precursors and differentiated cells in the inner neuroblast layer.
Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.
Distinct bands of expression were visible through the autopod and extending to the digit tips. Expression was strongest at regions of joint development, where attachment sites between connective tissue and the skeleton form.
Thick bands of expression radiated from the foot and along the digit borders. Expression was also visible at presumptive joint sites along the digits, but was not observed in the distal digit tips.
Thick bands of expression radiated from the foot and along the digit borders. Expression was also visible at presumptive joint sites along the digits, but was not observed in the distal digit tips.
The majority of tubules with ciliary expression are of ureteric-bud origin. Highest percentage of tubules of ureteric-bud origin have ciliary expression. There was a population of tubules without ciliary expression.
Strong signal was evident in differentiating tubular epithelial cells. There was a tendency for tubules that have formed a lumen to express more strongly than those without a lumen.
There is a sharp boundary of expression between the future right ventricle and the outflow tract, corresponding to the cranial extent of trabeculation. Expression was increased in the atrial trabeculae, particularly in the right.
The anteriorly located population directed towards the right (aortic side) of the outflow tract was less well developed and less organized in the mutant embryo compared to the wild type.
Strong expression was detected in the preodontoblasts and preameloblasts in the buccal cusp site and in the preodontoblasts and preameloblasts in the central groove region. Expression was reduced in the odontoblasts and ameloblasts in the lingual cusp.
Strong expression was detected in preodontoblasts and preameloblasts at the lateral side of the tooth germ. Weak expression was found in the preodontoblasts located at the presumptive cusps and the occlusal groove sites.
High expression was noted in the distal zeugopod surrounding and including the developing distal growth plates. Highest expression was restricted to distal end of both zeugopod bones and most proximal wrist bones.
Expression was more specific and restricted to the posterior regions of the limb and extended more prominently into the proximal zeugopod to include the olecranon. Expression was excluded from the developing distal growth plates.
Hindlimb innervation at E11.5 was variable. Staining was detected in this sample. When present, nerves in E11.5 hindlimb always extended for a shorter distance into the limb compared to the forelimb.
Expression is in one compartment located caudally, a negative compartment was located in the central part, and a compartment with sparse distribution of mildly positive Purkinje cells was located in the rostral part.
Expression is in one compartment located caudally, a negative compartment was located in the central part, and a compartment with sparse distribution of mildly positive Purkinje cells was located in the rostral part.
Expression was detected in the dorsal part of the lateral ganglionic eminence. The ventral limit of expression correlated with the ventral border for low, but not high, expression of Pax6.
Robust expression was noted in a high dorsal to low ventral gradient in the lateral ganglionic eminence. Weak or absent expression was in the ventral most region of the ventral lateral ganglionic eminence.
At E18.5, the expression gradient becomes restricted and largely confined to the dorsal lateral ganglionic eminence. Weak or absent expression was in the ventral most region of the ventral lateral ganglionic eminence.
Robust expression was noted in a high dorsal to low ventral gradient in the lateral ganglionic eminence. Weak or absent expression was in the ventral most region of the ventral lateral ganglionic eminence.
Expression was detected in a population of cells in the basal half of the ventricular zone near the ventricular zone/subventricular zone boundary in the lateral ganglionic eminence. There was minimal overlap with Sp8 staining.
Expression was detected in the ventricular and subventricular zones of both eminences. Signal stops a small distance from the lateral angle of the ventricle at the transition into the cortex.
Expression was absent from the apical cells, and thus from the surface mitoses of the ventricular zone, but was present in a band of cells that includes the basal, nonsurface mitotic precursors of the subventricular zone.
Number of expressing cells in dorsal one-fourth of lateral ganglionic eminence (dLGE) at level of rostral one-third of rostral-caudal axis was significantly lower for Hey1 knockout than for control.
