Staining was observed in myofibrils of varying stages of maturity, including nascent myofibrils with irregular and/or periodic dots of immunostaining with continuous F-actin staining. Mature, but very thin, myofibrils were also present at this stage, with regularly spaced dots of immunostaining alternating with distinct and periodic gaps in F-actin.
Expression was detected in the central region of the neural retina. Within the central retina, the most intense DCC expression was localized to the emerging inner nuclear layer. Weaker expression was seen in patches at the outer edge of the outer nuclear layer. No label was seen in the peripheral regions.
The intense expression domain above the outer nuclear layer expanded towards the periphery. In the most peripheral regions, chains of strongly expressing cells were situated directly below the region of accumulating postmitotic neurons. Within the E16.5 central retina, where differentiation was more advanced, the positive chains had disappeared.
Expression gradually disappeared in the border cells in the great epithelial ridge adjacent to the future organ of Corti, where the level of Sox2 distribution was gradually increased. Expression remained in the epithelial cells that will give rise to the inner sulcus cells where Sox2 is not expressed.
Expression gradually disappeared in the border cells in the great epithelial ridge adjacent to the future organ of Corti, where the level of Sox2 distribution was gradually increased. Expression remained in the epithelial cells that will give rise to the inner sulcus cells where Sox2 is not expressed.
The ophthalmic nerve was further defasciculated and widely spread in the frontal part. The distal parts of the maxillary andmandibular nerves were also defasciculated. The distal parts of the facial nerve was also expanded. No abnormal pathway or trajectory was observed in the oculomotor, trochlear, or vestibulocochlear nerves.
The ventromedial to dorsolateral extent of expression was normal. However, the three sclerotomes indicated display irregular and fused signals with altered rostrocaudal pattern. Strong signal in the rostral sclerotome halves, lateral to the negative spots. In the caudal sclerotome halves and ventromedially levels of expression are reduced.
Expressed in the central sclerotomes of the mature rostral somites as stripes along the rostral and caudal somite borders. In younger somites, expressed more evenly throughout the central sclerotome moieties. Clearly respected the somite borders. Expression was evenly distributed across the borderlines.
Authors state that expression is similar to Smad1. Strongly expressed in the cortex, in uninduced mesenchymal cells in the nephrogenic zone and in ureteric bud tips. A weaker signal was detected in mesenchymal cells in the medulla and around the ureter.
Tubules were less densely packed, with the interstitial space increased. Though in sections the number of Leydig cells appeared higher, the total number of Leydig cells was comparable with control mice when accounting for the small testis size of the mutant mice.
All airways down to segmental bronchi were strongly immunoreactive, while the distal branching buds were negative. Cytoplasmic staining was more and more prominent in the typical columnar cells of the lining epithelium in the proximal and medial parts of the bronchi, while the cuboidal epithelial cells in the distal, proliferating areas of the bronchi and bronchioli remained immunonegative.
All airways down to segmental bronchi were strongly immunoreactive, while the distal branching buds were negative. Cytoplasmic staining was more and more prominent in the typical columnar cells of the lining epithelium in the proximal and medial parts of the bronchi, while the cuboidal epithelial cells in the distal, proliferating areas of the bronchi and bronchioli remained immunonegative.
Expression is among the round chondrocytes, is stronger and more uniform in the columnar region in prehypertrophic and early hypertrophic chondrocytes, and is weaker in most of the hypertrophic region. Expression was reactivated in cells at the final stage of hypertrophy.
Expression was detected in radial precursors. The spatial domains of Fabp7 (BLBP) and Tubb3 immunopositive cells could not be completely separated. A substantial area was Fabp7-positive but Tubb3-negative. The area of the Fabp7 only immunopositive domain, relative to the total cerebellar area per section, was increased 1.8-fold in mutant cerebella at E13.5.
Expression was detected in the radial glia fibers. Consistent disruptions in the radial glia scaffold were observed across the cortex of the mutant. The web of staining normally associated with radial glia endfeet was substantially reduced in many regions of the mutant cortex.
Fewer immunopositive interneurons in mutant mice had entered the cortex from the medial ganglionic eminence than seen in control littermates. The distance traveled by immunopositive cells in the preplate and intermediate zone migratory streams was a significantly shorter distance in the mutant compared with controls.
Expression was mainly detected in the germinative zone of the developing cortex, especially the basal aspect. Most of the Esco2+ cells also expressed the intermediate progenitor cell marker Eomes, and to a lesser extent, cells in the transition stage between radial glial progenitor cells (Eomes+Pax6+), and in radial glial progenitor cells (Pax6+).
In the conditional mutant, expression was lower in the lateral area of the cortex. Co-expression with Casp3 and Pax6 indicated that increased apoptosis was mainly in intermediate progenitor cells (Eomes+) and and cells in transition stage between radial glia progenitor cells/intermediate progenitor cells (Eomes+/Pax6+).
In the conditional mutant, expression was lower in the lateral area of the cortex. Co-expression with Casp3 and Eomes indicated there was increased apoptosis in cells in transition stage between radial glia progenitor cells/intermediate progenitor cells (Eomes+/Pax6+) and in radial glial progenitor cells (Pax6+).
Expression was also observed in Casp3+ cells that were tdTomato negative, un-recombined cells that would notably include pyramidal cells, CGE-derived cortex inhibitory neurons, and glial cells. A small, but significant, difference in the number of Casp3+/tdTomato- cells was observed between genotypes at P0.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were found in both higher bins (3, 4) and lower bins (1, 2) of the cortical plate. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were found in both higher bins (3, 4) and lower bins (1, 2) of the cortical plate. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were found in both higher bins (3, 4) and lower bins (1, 2) of the cortical plate. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were found in both higher bins (3, 4) and lower bins (1, 2) of the cortical plate. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were found in both higher bins (3, 4) and lower bins (1, 2) of the cortical plate. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were found in both higher bins (3, 4) and lower bins (1, 2) of the cortical plate. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were found in both higher bins (3, 4) and lower bins (1, 2) of the cortical plate. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were found in both higher bins (3, 4) and lower bins (1, 2) of the cortical plate. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were found in both higher bins (3, 4) and lower bins (1, 2) of the cortical plate. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were found in both higher bins (3, 4) and lower bins (1, 2) of the cortical plate. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were found in both higher bins (3, 4) and lower bins (1, 2) of the cortical plate. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were found in both higher bins (3, 4) and lower bins (1, 2) of the cortical plate. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were found in both higher bins (3, 4) and lower bins (1, 2) of the cortical plate. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were found in both higher bins (3, 4) and lower bins (1, 2) of the cortical plate. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were spread out evenly throughout the cortical bins compared to controls. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were spread out evenly throughout the cortical bins compared to controls. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were spread out evenly throughout the cortical bins compared to controls. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were spread out evenly throughout the cortical bins compared to controls. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were spread out evenly throughout the cortical bins compared to controls. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were spread out evenly throughout the cortical bins compared to controls. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were spread out evenly throughout the cortical bins compared to controls. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were spread out evenly throughout the cortical bins compared to controls. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were spread out evenly throughout the cortical bins compared to controls. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were spread out evenly throughout the cortical bins compared to controls. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were spread out evenly throughout the cortical bins compared to controls. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were spread out evenly throughout the cortical bins compared to controls. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were spread out evenly throughout the cortical bins compared to controls. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
The distinct localization pattern of immunopositive neurons was not seen in MACF1;Nex-cre cerebral cortex. Immunopositive neurons in MACF1;Nex-cre mice were spread out evenly throughout the cortical bins compared to controls. Immunopositive neurons appeared abnormally spaced in the mutant compared with control.
Enhanced immunoreactivity for phosphorylated Mapk1/Mapk3 was detected in the corpus callosum of the conditional mutant compared to control. This was observed at all levels of the corpus callosum, including the lateral, medial, and caudal regions. The increase in staining was observed in the white matter and in distinct cells bodies (~30.1% increase compared with control cell bodies).
Enhanced immunoreactivity for phosphorylated Mapk1/Mapk3 was detected in the corpus callosum of the conditional mutant compared to control. This was observed at all levels of the corpus callosum, including the lateral, medial, and caudal regions. The increase in staining was observed in the white matter and in distinct cells bodies (~30.1% increase compared with control cell bodies).
Weak expression is detected in a small portion of the urethral epithelium of male closest to the opening. Expression is not detected in the phallic urethral epithelium at the base of the external genitalia. The distal urethral epithelium was not present in any of the sections examined.
Expression was detected in all BrdU labeled cells in the medial cortical wall in the upper portion of the ventricular zone (B) and arrayed in closer proximity to the ventricular zone of the region that will give rise to the dentate gyrus (C).
Expression was detected in Vim-immunopositive radial glial cells throughout the neuroepithelium and ventricular zone and in neuroblasts in the subventricular zone-intermediate zone. The second proliferative layer between the subventricular zone and primitive plexiform zone included cells that were immunopositive for Dclk1, Vim, and Dcx.
Expression was detected in radial glial cells throughout the neuroepithelium and ventricular zone, where there was overlap with Dclk1 expression. The second proliferative layer between the subventricular zone and primitive plexiform zone included cells that were immunopositive for Dclk1, Vim, and Dcx.
Expression was detected in the pallium, the subpallium, and the cortical hem. In the subpallium, most expression was localized in the medial ganglionic eminence, with sparse cells in the lateral ganglionic eminence. In the pallium, expression was found in the subventricular zone.
Much of prosomere 4 was negative. However, there was some expression in the archicortex up to the apex of the intraventricular convex curve. In prosomere 5, the neocortical and paleocortical plates, subplate and intermediate zones showed strong expression, while the ventricular zone does not.
Expression was detected in a gradient of high rostral to low caudal. Along the dorsoventral axis of the pallium, expression was detected in a high-dorsal to low-ventral gradient. The staining was complementary to that of Nr2f1 in both the rostrocaudal and dorsoventral axis.
Expression was detected in a gradient of high caudal to low rostral. Along the dorsoventral axis of the pallium, expression was detected in a high-ventral to low-dorsal gradient. The staining was complementary to that of Sp8 in both the rostrocaudal and dorsoventral axis.
No labelling was present in limbs of splotch mutants. MyoD expression was the same in axial and body wall muscles of wild-type and mutant embryos. Occasionally, a slight reduction of deep back muscles was seen at the level of the neural tube defect.
Expression in endothelial cells moving dorsoanteriorly toward head region. Obvious regionalization of expressing cells in the cardinal vein. Budding and spreading of expressing cells from the anterior cardinal vein was clearly polarized. Also expression in cells near esophagus and lung buds.
Expression in the ventral thalamus was expanded and appeared to fill all of the ventral thalamus. Expression was detected along the dorsal midline of the dorsal thalamus and pretectum, although its caudal limit did not form an expanded and sharp boundary.
Expression in the ventral thalamus was expanded and appeared to fill all of the ventral thalamus. Expression was detected along the dorsal midline of the dorsal thalamus and pretectum, although its caudal limit did not form an expanded and sharp boundary.
Expression was detected at both the alar and basal domains, although it was most intense along the boundary between these two domains. Signal entirely overlaps with the Cdh4 signal at the dorsal border of the ventral thalamus, except at the anteriormost position of the zona limitans intrathalamica.
Expression is in chiasmatic neurons in the caudal part. Expression extened at the chiasmatic midline and this population of neurons also extended laterally to the optic tract apposing the caudal border of the initial segment of the optic tract that was also positive.
In the optic pathway expression was weak in the stalk and the tract and the staining in the chiasm was reduced substantially in regions flanking both sides of the midline. Expression is in regions caudal to the chiasm, in a configuration mimicking the chiasmatic neurons.
Expression is in a strip of strongly labeled area, cut transversely in horizontal sections. In the optic pathway expression was weak in the stalk and the tract and the staining in the chiasm was reduced substantially in regions flanking both sides of the midline.
Expressed in the prethalamus, but was absent from the zona limitans intrathalamica (ZLI) and the mantle cells of the developing eminentia thalami. In the thalamus, expression levels were graded from low near the ZLI to high at the boundary with the pretectum.
Expressed around the junction of the optic nerve and the brain, with strongest expression dorsal to the site at which the optic stalk joins the diencephalon. In the more dorsal region, expression was some distance posterior to the axons. Also weakly expressed in a subset of the CD44/SSEA(Fut4) neurons.
c-myc is largely restricted to cells adjacent to the outside of the compact layer, and to isolated cells or clusters of cells adjacent to and on the inside of the compact layer. c-myc was also observed in endothelial cells lining the endocardial cushion.
The anterior boundary lay within the spinal cord and was at the level of the 5th prevertebra in the ventral part of the cord and the 3rd prevertebra in the middle of the cord. Irregularities in the border are consistent among different embryos.
Expression was in motor neurons. Expression in the visceral motor column was similar to wild type. Expression in the medial and lateral median motor columns of the mutant was significantly lower than in wild type. Graphed in Fig. 4A, 4C and 4D.
Expression was in motor neurons. Expression in the visceral motor column was similar to wild type. Expression in the medial and lateral median motor columns of the mutant was significantly lower than in wild type. Graphed in Fig. 4A, 4C and 4D.
Expression was in motor neurons. Expression in the visceral motor column was similar to wild type. Expression in the medial and lateral median motor columns of the mutant was significantly lower than in wild type. Graphed in Fig. 4A, 4C and 4D.
Expression was in motor neurons. Expression in the visceral motor column was similar to wild type. Expression in the medial and lateral median motor columns of the mutant was significantly lower than in wild type. Graphed in Fig. 4A, 4C and 4D.
Expression was in motor neurons. Expression in the visceral motor column was similar to wild type. Expression in the medial and lateral median motor columns of the mutant was significantly lower than in wild type. Graphed in Fig. 4A, 4C and 4D.
Expression was in motor neurons. Expression in the visceral motor column was similar to wild type. Expression in the medial and lateral median motor columns of the mutant was significantly lower than in wild type. Graphed in Fig. 4A, 4C and 4D.
Coexpression of Hoxc8 and Prph was found in a small subset of cells in the spinal cord at the outer border of the mantle layer. These cells are likely to be presynaptic motor neurons. Other sets of motor neurons expressed Hoxc8 alone.
Pax3 was expressed in streams of neural crest cells that could be seen emerging from rhombomeres 2 and 4, entering branchial arches 1 and 2 respectively. Streams of cells could also be seen emerging from the neural tube caudal to the otic vesicle.
Female embryos showed three patterns of Xist expression, the proportion of which changed over time. At E6.5, 1. a small fraction of the cells showed low level biallelic Xist expression. 2. a slightly larger proportion of cells displayed differential biallelic Xist expression. 3. in the majority of cells, abundant Xist RNA was detected from only one locus.
Female embryos showed three patterns of Xist expression, the proportion of which changed over time. At E7.0, 1. a small fraction of the cells showed low level biallelic Xist expression. 2. a slightly larger proportion of cells displayed differential biallelic Xist expression. 3. in the majority of cells, abundant Xist RNA was detected from only one locus.
Expressed in the anterior region. The domains of expression of Cer1 and Fgf8 were diametrically opposite to each other. The axis passing through the center of the two expression domains was almost parallel to both the long axis of the embryo and the left-right axis of the uterus.
Expressed in the anterior region. The domains of expression of Cer1 and Fgf8 were diametrically opposite to each other. The axis passing through the center of the two expression domains was almost parallel to both the long axis of the embryo and the left-right axis of the uterus.
Expressed in the posterior region. The domains of expression of Cer1 and Fgf8 were diametrically opposite to each other. The axis passing through the center of the two expression domains was almost parallel to both the long axis of the embryo and the left-right axis of the uterus.
Expressed in the posterior region. The domains of expression of Cer1 and Fgf8 were diametrically opposite to each other. The axis passing through the center of the two expression domains was almost parallel to both the long axis of the embryo and the left-right axis of the uterus.
Authors state that expression is similar to Smad1. Strongly expressed in the cortex, in uninduced mesenchymal cells in the nephrogenic zone and in ureteric bud tips. A weaker signal was detected in mesenchymal cells in the medulla and around the ureter.
At P12, Anxa4 was expressed in fewer hair cells, which were labeled by the pan-hair cell marker Myo7a. Based on hair cell morphology, greater than 65% of Type II hair cells were Anxa4-immunopositive, with a decreasing expression in Type I hair cells.
At P12, Anxa4 was expressed in fewer hair cells, which were labeled by the pan-hair cell marker Myo7a. Based on hair cell morphology, greater than 65% of Type II hair cells were Anxa4-immunopositive, with a decreasing expression in Type I hair cells.
At P64, Anxa4 was expressed in fewer hair cells, which were labeled by the pan-hair cell marker Myo7a. Based on hair cell morphology, greater than 65% of Type II hair cells were Anxa4-immunopositive, with a decreasing expression in Type I hair cells.
At P64, Anxa4 was expressed in fewer hair cells, which were labeled by the pan-hair cell marker Myo7a. Based on hair cell morphology, greater than 65% of Type II hair cells were Anxa4-immunopositive, with a decreasing expression in Type I hair cells.
Expression was noted in a patch of cells dorsal to the first branchial arch in the region of the neural tube. These cells are in the region of the primordia of the trigeminal ganglion and may be either placodal and/or neural crest derived cells.
Expressed in the epithelial component of the juxta-oral organ, which located on the anterior fascia of the temporal muscle and curved steeply inwards running posterio-medially to reach the soft palate. The junction of the anterior and middle portions was observed in the posterior wall of the orbit.
Expressed in the epithelial component of the juxta-oral organ. The posterior portion was located in the soft palate posterior to the palatine bone and lateral to the pterygoid bone. The growth of the posterior part on the right and left sides differs in this case.
Expressed in the juxta-oral organ. The anterior portion was located at the antero-inferior margin of the superficial masseter. It was observed between the mandible and the fascia of the superficial masseter muscle and is parallel to the facial artery and vein.
Expression was detected in the ventricular zone in 2 clusters on either side of the ventrodorsal midline, restricted to the outer 1/2 to 1/4 of the ventricular zone. No detectable hybridization in the most ventral and dorsal regions of ventricular zone.
The oculomotor nerve maintained a plexus in the central location with Pitx2+ cells, which was devoid of myofibers as marked by myosin heavy chain antibody. The nerve did form small dorsal axon projections to initiate the superior branch and likely the branch to the inferior oblique.
Expression was detected in trophoblast giant cells located on the border between the labyrinth and junctional zone. Expression in glycogen trophoblast cells was less strong than at E9.5, and seemed most intense in the cells located on the basal side of the junctional zone.
Expression was detected in Vim-immunopositive radial glial cells throughout the neuroepithelium and ventricular zone and in neuroblasts in the subventricular zone-intermediate zone. The second proliferative layer between the subventricular zone and primitive plexiform zone included cells that were immunopositive for Dclk1, Vim, and Dcx.
Expression was detected in radial glial cells throughout the neuroepithelium and ventricular zone, where there was overlap with Dclk1 expression. The second proliferative layer between the subventricular zone and primitive plexiform zone included cells that were immunopositive for Dclk1, Vim, and Dcx.
Shh is expressed at the mesial tip of the jaw in a short, thin strip of epithelial cells that extends distally. Shh is also expressed further distally at the site of amalgamation. Expression was symmetrical on either side of the median sulcus.
Nuclear expression diminished in intensity with gradually more nuclei becoming unlabeled in late cleavage. The continuous linear staining pattern increased in early blastocyst stage. Mid-plane section of early blastocysts was seen as punctuate dots at the apicolateral membrane regions of outer cells.
