In the tibial growth plate at suckling stages (postnatal week 1), immunopositive cells at the chondro-osseus junction were increased in number; and the distance between the immunoreactive cells was shorter than that in newborns. The number of septoclasts increased in number from E16 to postnatal week 1-2.
In the tibial growth plate at suckling stages (postnatal week 2), immunopositive cells at the chondro-osseus junction were increased in number; and the distance between the immunoreactive cells was shorter than that in newborns. The number of septoclasts increased in number from E16 to postnatal week 1-2, and the peak number was reached at postnatal week 2.
Expression was detected in most chondrocytes of the upper and peripheral resting zone of the epiphysis, and to some extent in the laterally located proliferating chondrocytes. Signal was absent from the hypertrophic zone, the central part of the proliferating zone and the joint surface. Strongest expression resided in the periphery of the epiphyses.
Expression was significantly down-regulated in the cranial mesenchyme except in the meningeal layer. Expression gradually faded on the lateral side of the head concomitant with the expansion of the calvarial bone into this region, while strong expression was maintained in the mesenchyme of the cranial sutures at the dorsal midline.
Expression was significantly down-regulated in the cranial mesenchyme except in the meningeal layer. Expression gradually faded on the lateral side of the head concomitant with the expansion of the calvarial bone into this region, while strong expression was maintained in the mesenchyme of the cranial sutures at the dorsal midline.
Expression was detected in the meningeal layer of supraorbital mesenchyme (SOM), and in the dermal layer at the parietal bone level. Zic1 expression was not found in the calvarial anlage within the SOM, which includes the osteogenic mesenchyme for the frontal bone and the parietal bone, and the prospective coronal suture in between.
At postnatal week 4, lacZ staining was still found in the region of the olfactory epithelium that covers the complex turbinate structures in the posterior nasal cavity. A large part of the chemosensory surface was stained, extending along the anterior-posterior axis of the nose. Only the dorsal part close to the cribriform plate contained expressing cells, whereas the more ventral domain was devoid of staining.
Graded expression was detected in layer 4 extending across most of the rostrocaudal extent of the neocortex; expression is strong rostrally and declines to very low levels near the extreme caudal pole of the neocortex. Layer 5 is also differentially expressed, with high expression restricted to the rostral pole and a rapid decline to very low levels at the midpoint of the neocortex. Expression was very low or nondetectable in the caudal half of the neocortex.
Graded expression was detected in layer 4 extending across most of the rostrocaudal extent of the neocortex; expression is strong rostrally and declines to very low levels near the extreme caudal pole of the neocortex. Layer 5 is also differentially expressed, with high expression restricted to the rostral pole and a rapid decline to very low levels at the midpoint of the neocortex. Expression was very low or nondetectable in the caudal half of the neocortex.
Broad expression was detected across the neocortex. Strong expression was in layer 5 in intermediate and caudal parts of the neocortex, but was weak in rostral parts. The strong to weak expression occurs abruptly at a position that corresponds to the boundary between motor and somatosensory areas. Strong expression was also detected rostrally in layers 2/3, but in intermediate parts of the neocortex, the expression declines rapidly to low or nondetectable.
Broad expression was detected across the neocortex. Strong expression was in layer 5 in intermediate and caudal parts of the neocortex, but was weak in rostral parts. The strong to weak expression occurs abruptly at a position that corresponds to the boundary between motor and somatosensory areas. Strong expression was also detected rostrally in layers 2/3, but in intermediate parts of the neocortex, the expression declines rapidly to low or nondetectable.
Bright chains of closely opposed expressing cells were observed extending from the outer surface of the outer nuclear layer through to the expanding inner nuclear layer in the central region of the retina. The domain of high DCC mRNA expression in the inner nuclear layer had expanded towards the periphery in a decreasing gradient. The region of expression was more extensive in the temporal side of the developing retina compared to the nasal region.
Expression was graded from base to tip in mural mitral leaflet and the aortic leaflet of the mitral valve with higher levels detected in the base and mid regions of the leaflet. Additionally, subendothelial fibroblasts on the atrial aspect of the valve leaflets exhibit the highest level of expression. At this stage, very weak expression was detected in the ventricularis at the tips of the mitral leaflets.
The ESCO2cKO mutant cortex demonstrated increased apoptosis. It appeared milder in the lateral area of the cortex than in the medial or dorsal areas. The mutant lateral cortex apoptotic cells were mostly found in the basal side. Co-immunostaining indicated that increased apoptosis was mainly in intermediate progenitor cells (Eomes+) and and cells in transition stage between radial glia progenitor cells/intermediate progenitor cells (Eomes+/Pax6+). A lesser extent of apoptosis was seen in radial glia progenitor cells (Pax6+).
Expression in the subpallium formed two continuums. One was located between the ganglionic eminence and the ventral pial surface; these cells are likely to be olfactory tubercle neuron precursors migrating from the lateral ganglionic eminence. The second continuum of stained cells was positioned between the medial ganglionic eminence and the ventral cortex, suggestive of cortical interneurons generated in the medial ganglionic eminence and migrating towards the cortex.
Anterior boundary of expression in both central nervous system and somites was more caudal compared to that of the 1.8 kb transcript detected by probe B (see assay MGI:5909948). The anterior boundary of expression in somites was at somite 13-14. The limit of expression in somitic mesoderm was off set caudally compared to that of the neural tube, with an interval of about 10 somites.
Anterior boundary of expression in both central nervous system and somites was more caudal compared to that of the 1.8 kb transcript detected by probe B (see assay MGI:5909948). The anterior boundary of expression in somites was at somite 13-14. The limit of expression in somitic mesoderm was off set caudally compared to that of the neural tube, with an interval of about 10 somites.
In the growth plate at sub-adult stage (postnatal week 9), numerous immunopositive cells were arranged along the chondro-osseus junction with a distribution pattern similar to that at the postnatal week 3 or 4 stage, but a decrease in number of immunoreactive cells with a distribution at wider distances from one another was clearly observed. The number of septoclasts at postnatal week 9 decreased to about 40% of the number found at postnatal week 2.
