The DASH complex is a ~10 subunit microtubule-binding complex that is transferred to the kinetochore prior to mitosis []. In Saccharomyces cerevisiae (Baker's yeast) DASH forms both rings and spiral structures on microtubules in vitro [, ]. Components of the DASH complex, including Dam1, Duo1, Spc34, Dad1 and Ask1, are essential and connect the centromere to the plus end of spindle microtubules []. Throughout the cell cycle Dad1 remains bound to kinetochores and its association is dependent on the Mis6 and Mal2 [].
The DASH complex is a ~10 subunit microtubule-binding complex that is transferred to the kinetochore prior to mitosis []. In Saccharomyces cerevisiae (Baker's yeast) DASH forms both rings and spiral structures on microtubules in vitro [, ]. Components of the DASH complex, including Dam1, Duo1, Spc34, Dad1 and Ask1, are essential and connect the centromere to the plus end of spindle microtubules [].
The DASH complex is a ~10 subunit microtubule-binding complex that is transferred to the kinetochore prior to mitosis []. In Saccharomyces cerevisiae (Baker's yeast) DASH forms both rings and spiral structures on microtubules in vitro [, ]. Components of the DASH complex, including Dam1, Duo1, Spc34, Dad1 and Ask1, are essential and connect the centromere to the plus end of spindle microtubules [].
The DASH complex is a ~10 subunit microtubule-binding complex that is transferred to the kinetochore prior to mitosis []. In Saccharomyces cerevisiae (Baker's yeast) DASH forms both rings and spiral structures on microtubules in vitro [, ]. Components of the DASH complex, including Dam1, Duo1, Spc34, Dad1 and Ask1, are essential and connect the centromere to the plus end of spindle microtubules [].
Fission yeast has three kinetochore protein complexes. Two complexes, Sim4 and Ndc80-MIND-Spc7 (NMS), are constitutive components, whereas the third complex, DASH, is transiently associated with kinetochores only in mitosis and is required for precise chromosome segregation. The Sim4 complex functions as a loading dock for the DASH complex. Sim4 consists of a number of different proteins including Ftas 1-7 and Dad1 [].This entry represents Fta2.
Fission yeast has three kinetochore protein complexes. Two complexes, Sim4 and Ndc80-MIND-Spc7 (NMS), are constitutive components, whereas the third complex, DASH, is transiently associated with kinetochores only in mitosis and is required for precise chromosome segregation. The Sim4 complex functions as a loading dock for the DASH complex. Sim4 consists of a number of different proteins including Ftas 1-7 and Dad1 [].This entry represents Fta4.
This entry represents centromere protein O (CENP-O) and its homologues in yeasts, Mcm21 and Mal2. In humans, centromere protein O (CENP-O) is a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation []. CENP-O mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. CENP-O modulates the kinetochore-bound levels of NDC80 complex []. It may be involved in incorporation of newly synthesized CENP-A into centromeres via its interaction with the CENPA-NAC complex [].In Saccharomyces cerevisiae, Mcm21 is a component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p), which links kinetochore subunits with subunits bound to microtubules during kinetochore assembly [, ]. In Schizosaccharomyces pombe, Mal2 is a component of the Sim4 complex, which is required for loading the DASH complex onto the kinetochore via interaction with Dad1 []. It plays a role in the maintenance of core chromatin structure and kinetochore function [].
This family includes Chl4 from budding yeasts, mis15 from fission yeasts and centromere protein N (CENP-N) from animals. In Saccharomyces cerevisiae, Chl4 is an outer kinetochore structural component required for chromosome stability []. Chl4 is a component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p []. It is required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation []. In Schizosaccharomyces pombe, mis15 is a subunit of the Sim4 complex, which is required for loading the DASH complex onto the kinetochore via interaction with dad1 []. It is required for correct chromosome segregation where it has a role in the formation and/or maintenance of specialised chromatin at the centromere []. In humans, centromere protein N (CENP-N) is a component of the CENPA-NAC (nucleosome-associated) complex, which plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation []. CENP-N localises exclusively in the kinetochore domain of centromeres []. It is required for chromosome congression and efficiently align the chromosomes on a metaphase plate [].
