This entry includes Gde1 from yeasts and GPCPD1 from mammals. Gde1 is a glycerophosphocholine glycerophosphodiesterase responsible for the hydrolysis of intracellular glycerophosphocholine into glycerol-phosphate and choline [, ].GPCPD1 (also known as GDE5) controls skeletal muscle development via a non-enzymatic mechanism [].
This entry represents the GP-PDE domain.The glycerophosphodiester phosphodiesterases (GD-PDEs) were initiallycharacterised in bacteria, where they have functional roles for production ofmetabolic carbon and phosphate sources from glycerophosphodiesters and inadherence to and degradation of mammalian host-cell membranes. Mammalian GP-GDEs have been identified more recently and shown to be implicated in severalphysiological functions. GD-PDEs are involved in glycerol metabolism andcatalyze the reaction of glycerophosphodiester and water to alcohol andsn-glycerol-3-phosphate. They display broad specificity forglycerophosphodiesters, such as glycerophosphocholine,glycerophosphoethanolamine, glycerophosphoglycerol andbis(glycerophosphoglycerol).The GP-PDE domain adopts the ubiquitous triosephosphate isomerase (TIM) barrelalpha/beta fold. The TIM barrel iscomprised of an eight-stranded parallel β-sheet barrel surrounded by eightα-helices. There is a small insertion to the conventional TIM barrelstructure referred to as the GDPD-insertion (GDPD-I). The GDPD-I is comprisedof beta strands, α-helices (H3 and H4), and 3/10 helices. Although the TIMbarrel and a small insertion are unique for GP-PDE family, there are subtledifferences in size and topology of each domain [, ].Some proteins known to contain a GP-PDE domain are listed below:Bacterial glycerophosphoryl diester phosphodiesterase GlpQ (EC 3.1.4.46).Bacterial gylcerophosphoryl diester phosphodiesterase UgpQ (EC 3.1.4.46).Mammalian glycerophosphodiester phosphodiesterase 1 (GDE1) (EC 3.1.4.44)(or MMIR16) [], an integral membrane glycoprotein that interacts withregulator of G protein signaling proteins. It hydrolyzesglycerophosphoinositols (GPIs) producing inositol and glycerol 3-phosphate.Mammalian glycerophosphodiester phosphodiesterase domain-containing protein5 (GDPD5) (EC 3.1.-.-) (or GDE2) [].Mammalian glycerophosphoinositol inositolphosphodiesterase GDPD2 (or GDE3)(EC 3.1.4.43) [], up-regulated during osteoblast differentiation and canaffect cell morpholgy. It hydrolyzes glycerophosphoinositol (GroPIns),producing inositol 1-phosphate and glycerol.Mammalian glycerophosphodiester phosphodiesterase domain-containing protein1 (GDPD1) (EC 3.1.-.-) (or GDE4) [].Mammalian glycerophosphocholine phosphodiesterase GPCPD1 (EC 3.1.4.2) (orGDE5), selectively hydrolyzes glycerophosphocholine (GroPCho) and controlsskeletal muscle development [].Mammalian glycerophosphodiester phosphodiesterase domain-containing protein4 (GDPD4) (EC 3.1.-.-) (or GDE6) [].
