Nab2 is a yeast heterogeneous nuclear ribonucleoprotein that modulates poly(A) tail length and mRNA. This is the N-terminal domain of the protein which mediates interactions with the C-terminal globular domain, Myosin-like protein 1 and the mRNA export factor, Gfd1 []. The N-terminal domain of Nab2 shows a structure of a helical fold. The N-terminal domain of Nab2 is thought to mediate protein:protein interactions that facilitate the nuclear export of mRNA []. An essential hydrophobic Phe73 patch on the N-terminal domain is thought to be an important component of the interface between Nab2 and Mlp1 [].
Nab1 and Nab2 are co-repressors that specifically interact with and repress transcription mediated by the three members of the NGFI-A (Egr-1, Krox24, zif/268) family of eukaryotic (metazoa) transcription factors []. This C-terminal region is found only in the Nab1 subfamily.
Nab1 and Nab2 are co-repressors that specifically interact with and repress transcription mediated by the three members of the NGFI-A (Egr-1, Krox24, zif/268) family of eukaryotic (metazoa) transcription factors []. This entry represents the N-terminal NAB domain, which interacts with the EGR1 inhibitory domain (R1) []. It may also mediate multimerisation.
Nab1 and Nab2 are co-repressors that specifically interact with and repress transcription mediated by the three members of the NGFI-A (Egr-1, Krox24, zif/268) family of eukaryotic (metazoa) transcription factors []. This entry represents a NAB domain 2 of the protein. It is necessary for transcriptional repression by the Nab proteins []. It is also required for transcription activation by Nab proteins at Nab-activated promoters [].
Nab1 and Nab2 are co-repressors that specifically interact with and repress transcription mediated by the three members of the NGFI-A (Egr-1, Krox24, zif/268) family of eukaryotic (metazoa) transcription factors []. This entry represents a NAB domain 2 of the protein. It is necessary for transcriptional repression by the Nab proteins []. It is also required for transcription activation by Nab proteins at Nab-activated promoters [].
Proteins in this family are transcriptional regulators, including Nab (NGFI-A-binding protein) from Drosophila and Nab1/2 from mammals. Nab1 and Nab2 are co-repressors that specifically interact with and repress transcription mediated by the three members of the NGFI-A (Egr-1, Krox24, zif/268) family of eukaryotic (metazoa) transcription factors []. Drosophila Nab controls the activity of zinc-finger transcription factors, such as Squeeze and Rotund, in Drosophila development [].
Budding yeast Nab2 is a nuclear polyadenylated RNA-binding protein that contains seven CCCH-type zinc fingers and an RGG box []. It is required for nuclear mRNA export and poly(A) tail length control [, ]. The tertiary structure has been solved and the protein is all helical (). Its animal homologue, known as ZC3H14, has been shown to control poly(A) tail length in neuronal cells [].
This superfamily represents the N-terminal domain of the nuclear abundant poly(A) RNA-bind protein 2 (Nab2), a yeast heterogeneous nuclear ribonucleoprotein that modulates poly(A) tail length and mRNA. This domain can also be found in Its animal homologue, ZC3H14, which has been shown to control poly(A) tail length in neuronal cells []. The N-terminal domain mediates interactions with the C-terminal globular domain, Myosin-like protein 1 and the mRNA export factor, Gfd1. An essential hydrophobic Phe73 patch on the N-terminal domain is thought to be an important component of the interface between Nab2 and Mlp1 [].
