Enhancer of filamentation 1 (also known as NEDD9 or Cas-L) is a member of the CAS family. It is a scaffolding protein that assembles signaling complexes regulating multiple cellular processes, such as cell adhesion, migration, invasion, and metastasis. It is commonly dysregulated during cancer progression. It interacts with Aurora-A kinase to control ciliary resorption, and with Src and other partners to influence proliferative signaling pathways often activated in autosomal dominant polycystic kidney disease [].CAS (Crk-associated substrate) family members are adaptor proteins that contain a highly conserved N-terminal SH3 domain, an adjacent unstructured domain (substrate domain) containing multiple tyrosine phosphorylation sites that enable binding by SH2-domain containing proteins, a serine-rich four-helix bundle, and a FAT-like C-terminal domain. Most of these domains mediate protein-protein interactions. Through these interactions, they assemble larger signaling complexes that are essential for cell proliferation, survival, migration, and other processes []. The CAS family consists of four members: BCAR1, HEF1, EFS, and CASS4 [].
