This entry includes tRNA A64-2'-O-ribosylphosphate transferase Rit1 from budding yeasts. Rit1 is a phospho-ribosyl transferase that exclusively modifies the initiator tRNA (tRNAMet(i)) by the addition of a 2'-O-ribosyl phosphate group to Adenosine 64 [, ]. This entry also includes the C3F10.06c protein from Schizosaccharomyces pombe.
Rit1 modifies exclusively the initiator tRNA in position 64 using 5'-phosphoribosyl-1'-pyrophosphate as the modification donor. As the initiator tRNA participates both in the initiation and elongation of translation, the 2'-O-ribosyl phosphate modification discriminates the initiator tRNAs from the elongator tRNAs []. This C-terminal domain shows similarity to dual specificity phosphatases (DUSPs) [].
This entry represents the N-terminal domain of the enzyme Rit1 (tRNA A64-2'-O-ribosylphosphate transferase) which modifies exclusively the initiator tRNA in position 64 using 5'-phosphoribosyl-1'-pyrophosphate as the modification donor. As the initiator tRNA participates both in the initiation and elongation of translation, the 2'-O-ribosyl phosphate modification discriminates the initiator tRNAs from the elongator tRNAs []. The N-terminal domain is the most conserved region of the protein [].
