RING finger protein 152 (RNF152) has E3 ligase activity involved in lysosome-related apoptosis []. It is a negative regulator of the mTORC1 pathway by targeting RagA for K63-linked ubiquitination [].
This entry represents the C-terminal domain of the E3 ubiquitin ligase RNF152. This hydrophobic transmembrane domain is responsible for the lysosomal localisation of the protein as well as its ubiquitination activity. RNF152 polyubiquitinates several proteins and itself, which leads to proteasome degradation. It has been shown that this protein has also pro-apoptotic activity, dependent on its RING finger and transmembrane domains []. In addition, the transmembrane domain of RNF152 is necessary for negative regulation of the mTORC1 pathway in an amino-acid-sensitive manner, as it mediates RNF152-RagA interaction and RagA ubiquitination [].
